Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing

Abstract Patients with diabetes experience delayed wound healing because of the uncontrolled glucose level in their bloodstream, which leads to impaired function of white blood cells, poor circulation, decreased production and repair of new blood vessels. Treatment using polydeoxyribonucleotide (PDR...

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Autores principales:	Da Yong Shin, Ji-Ung Park, Min-Ha Choi, Sukwha Kim, Hyoun-Ee Kim, Seol-Ha Jeong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/10a620102bcc46ecbf25c229e255736d
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spelling	oai:doaj.org-article:10a620102bcc46ecbf25c229e255736d2021-12-02T18:36:13ZPolydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing10.1038/s41598-020-74004-02045-2322https://doaj.org/article/10a620102bcc46ecbf25c229e255736d2020-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-74004-0https://doaj.org/toc/2045-2322Abstract Patients with diabetes experience delayed wound healing because of the uncontrolled glucose level in their bloodstream, which leads to impaired function of white blood cells, poor circulation, decreased production and repair of new blood vessels. Treatment using polydeoxyribonucleotide (PDRN), which is a DNA extracted from the sperm cells of salmon, has been introduced to accelerate the healing process of diabetic wounds. To accelerate the wound-healing process, sustained delivery of PDRN is critical. In this study, taking advantage of the non-invasive gelation property of alginate, PDRN was loaded inside the hydrogel (Alg-PDRN). The release behavior of PDRN was altered by controlling the crosslinking density of the Alg hydrogel. The amount of PDRN was the greatest inside the hydrogel with the highest crosslinking density because of the decreased diffusion. However, there was an optimal degree of crosslinking for the effective release of PDRN. In vitro studies using human dermal fibroblasts and diabetes mellitus fibroblasts and an in ovo chorioallantoic membrane assay confirmed that the Alg-PDRN hydrogel effectively induced cell proliferation and expression of angiogenic growth factors and promoted new blood vessel formation. Its effectiveness for accelerated diabetic wound healing was also confirmed in an in-vivo animal experiment using a diabetic mouse model.Da Yong ShinJi-Ung ParkMin-Ha ChoiSukwha KimHyoun-Ee KimSeol-Ha JeongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Da Yong Shin Ji-Ung Park Min-Ha Choi Sukwha Kim Hyoun-Ee Kim Seol-Ha Jeong Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing
description	Abstract Patients with diabetes experience delayed wound healing because of the uncontrolled glucose level in their bloodstream, which leads to impaired function of white blood cells, poor circulation, decreased production and repair of new blood vessels. Treatment using polydeoxyribonucleotide (PDRN), which is a DNA extracted from the sperm cells of salmon, has been introduced to accelerate the healing process of diabetic wounds. To accelerate the wound-healing process, sustained delivery of PDRN is critical. In this study, taking advantage of the non-invasive gelation property of alginate, PDRN was loaded inside the hydrogel (Alg-PDRN). The release behavior of PDRN was altered by controlling the crosslinking density of the Alg hydrogel. The amount of PDRN was the greatest inside the hydrogel with the highest crosslinking density because of the decreased diffusion. However, there was an optimal degree of crosslinking for the effective release of PDRN. In vitro studies using human dermal fibroblasts and diabetes mellitus fibroblasts and an in ovo chorioallantoic membrane assay confirmed that the Alg-PDRN hydrogel effectively induced cell proliferation and expression of angiogenic growth factors and promoted new blood vessel formation. Its effectiveness for accelerated diabetic wound healing was also confirmed in an in-vivo animal experiment using a diabetic mouse model.
format	article
author	Da Yong Shin Ji-Ung Park Min-Ha Choi Sukwha Kim Hyoun-Ee Kim Seol-Ha Jeong
author_facet	Da Yong Shin Ji-Ung Park Min-Ha Choi Sukwha Kim Hyoun-Ee Kim Seol-Ha Jeong
author_sort	Da Yong Shin
title	Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing
title_short	Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing
title_full	Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing
title_fullStr	Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing
title_full_unstemmed	Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing
title_sort	polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/10a620102bcc46ecbf25c229e255736d
work_keys_str_mv	AT dayongshin polydeoxyribonucleotidedeliveringtherapeutichydrogelfordiabeticwoundhealing AT jiungpark polydeoxyribonucleotidedeliveringtherapeutichydrogelfordiabeticwoundhealing AT minhachoi polydeoxyribonucleotidedeliveringtherapeutichydrogelfordiabeticwoundhealing AT sukwhakim polydeoxyribonucleotidedeliveringtherapeutichydrogelfordiabeticwoundhealing AT hyouneekim polydeoxyribonucleotidedeliveringtherapeutichydrogelfordiabeticwoundhealing AT seolhajeong polydeoxyribonucleotidedeliveringtherapeutichydrogelfordiabeticwoundhealing
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Polydeoxyribonucleotide-delivering therapeutic hydrogel for diabetic wound healing

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