Integrated analysis of mRNA and miRNA expression in HeLa cells expressing low levels of Nucleolin

Abstract Nucleolin is an essential protein that plays important roles in the regulation of cell cycle and cell proliferation. Its expression is up regulated in many cancer cells but its molecular functions are not well characterized. Nucleolin is present in the nucleus where it regulates gene expres...

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Autores principales:	Sanjeev Kumar, Elizabhet Cruz Gomez, Mounira Chalabi-Dchar, Cong Rong, Sadhan Das, Iva Ugrinova, Xavier Gaume, Karine Monier, Fabien Mongelard, Philippe Bouvet
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/10a72c2701d349e4b3c802e0984c0e05
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spelling	oai:doaj.org-article:10a72c2701d349e4b3c802e0984c0e052021-12-02T12:32:01ZIntegrated analysis of mRNA and miRNA expression in HeLa cells expressing low levels of Nucleolin10.1038/s41598-017-09353-42045-2322https://doaj.org/article/10a72c2701d349e4b3c802e0984c0e052017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09353-4https://doaj.org/toc/2045-2322Abstract Nucleolin is an essential protein that plays important roles in the regulation of cell cycle and cell proliferation. Its expression is up regulated in many cancer cells but its molecular functions are not well characterized. Nucleolin is present in the nucleus where it regulates gene expression at the transcriptional and post-transcriptional levels. Using HeLa cells depleted in nucleolin we performed an mRNA and miRNA transcriptomics analysis to identify biological pathways involving nucleolin. Bioinformatic analysis strongly points to a role of nucleolin in lipid metabolism, and in many signaling pathways. Down regulation of nucleolin is associated with lower level of cholesterol while the amount of fatty acids is increased. This could be explained by the decreased and mis-localized expression of the transcription factor SREBP1 and the down-regulation of enzymes involved in the beta-oxidation and degradation of fatty acids. Functional classification of the miRNA-mRNA target genes revealed that deregulated miRNAs target genes involved in apoptosis, proliferation and signaling pathways. Several of these deregulated miRNAs have been shown to control lipid metabolism. This integrated transcriptomic analysis uncovers new unexpected roles for nucleolin in metabolic regulation and signaling pathways paving the way to better understand the global function of nucleolin within the cell.Sanjeev KumarElizabhet Cruz GomezMounira Chalabi-DcharCong RongSadhan DasIva UgrinovaXavier GaumeKarine MonierFabien MongelardPhilippe BouvetNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-17 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sanjeev Kumar Elizabhet Cruz Gomez Mounira Chalabi-Dchar Cong Rong Sadhan Das Iva Ugrinova Xavier Gaume Karine Monier Fabien Mongelard Philippe Bouvet Integrated analysis of mRNA and miRNA expression in HeLa cells expressing low levels of Nucleolin
description	Abstract Nucleolin is an essential protein that plays important roles in the regulation of cell cycle and cell proliferation. Its expression is up regulated in many cancer cells but its molecular functions are not well characterized. Nucleolin is present in the nucleus where it regulates gene expression at the transcriptional and post-transcriptional levels. Using HeLa cells depleted in nucleolin we performed an mRNA and miRNA transcriptomics analysis to identify biological pathways involving nucleolin. Bioinformatic analysis strongly points to a role of nucleolin in lipid metabolism, and in many signaling pathways. Down regulation of nucleolin is associated with lower level of cholesterol while the amount of fatty acids is increased. This could be explained by the decreased and mis-localized expression of the transcription factor SREBP1 and the down-regulation of enzymes involved in the beta-oxidation and degradation of fatty acids. Functional classification of the miRNA-mRNA target genes revealed that deregulated miRNAs target genes involved in apoptosis, proliferation and signaling pathways. Several of these deregulated miRNAs have been shown to control lipid metabolism. This integrated transcriptomic analysis uncovers new unexpected roles for nucleolin in metabolic regulation and signaling pathways paving the way to better understand the global function of nucleolin within the cell.
format	article
author	Sanjeev Kumar Elizabhet Cruz Gomez Mounira Chalabi-Dchar Cong Rong Sadhan Das Iva Ugrinova Xavier Gaume Karine Monier Fabien Mongelard Philippe Bouvet
author_facet	Sanjeev Kumar Elizabhet Cruz Gomez Mounira Chalabi-Dchar Cong Rong Sadhan Das Iva Ugrinova Xavier Gaume Karine Monier Fabien Mongelard Philippe Bouvet
author_sort	Sanjeev Kumar
title	Integrated analysis of mRNA and miRNA expression in HeLa cells expressing low levels of Nucleolin
title_short	Integrated analysis of mRNA and miRNA expression in HeLa cells expressing low levels of Nucleolin
title_full	Integrated analysis of mRNA and miRNA expression in HeLa cells expressing low levels of Nucleolin
title_fullStr	Integrated analysis of mRNA and miRNA expression in HeLa cells expressing low levels of Nucleolin
title_full_unstemmed	Integrated analysis of mRNA and miRNA expression in HeLa cells expressing low levels of Nucleolin
title_sort	integrated analysis of mrna and mirna expression in hela cells expressing low levels of nucleolin
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/10a72c2701d349e4b3c802e0984c0e05
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Integrated analysis of mRNA and miRNA expression in HeLa cells expressing low levels of Nucleolin

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