Epigenome-wide methylation differences in a group of lean and obese women – A HUNT Study

Abstract Knowledge of epigenetically regulated biomarkers linked to obesity development is still scarce. Improving molecular understanding of the involved factors and pathways would improve obesity phenotype characterization and reveal potentially relevant targets for obesity intervention. The Illum...

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Autores principales:	Kirsti Kvaløy, Christian Magnus Page, Turid Lingaas Holmen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Differentially Methylated Sites Epigenome-wide Association Studies (EWAS) Obesity Development Test Construction (TEX41) Obesity Susceptibility Medicine R Science Q
Acceso en línea:	https://doaj.org/article/10b4271b851f45b8ad0dc658aa1e06e7
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spelling	oai:doaj.org-article:10b4271b851f45b8ad0dc658aa1e06e72021-12-02T15:07:45ZEpigenome-wide methylation differences in a group of lean and obese women – A HUNT Study10.1038/s41598-018-34003-82045-2322https://doaj.org/article/10b4271b851f45b8ad0dc658aa1e06e72018-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34003-8https://doaj.org/toc/2045-2322Abstract Knowledge of epigenetically regulated biomarkers linked to obesity development is still scarce. Improving molecular understanding of the involved factors and pathways would improve obesity phenotype characterization and reveal potentially relevant targets for obesity intervention. The Illumina Infinium HumanMethylation450 BeadChip was used in a leucocyte epigenome-wide association study (EWAS) to quantify differential DNA methylation in 60 lean compared with 60 obese young women. Replication was done in monozygotic twins discordant for obesity. At adolescence and adulthood, the two weight groups differed significantly in obesity-related traits and metabolic risk factors. Differential hypomethylation was overrepresented in obese compared to lean women. In the adjusted model, the EWAS revealed 10 differentially methylated CpG sites linked to 8 gene loci – COX6A1P2/FGD2, SBNO2, TEX41, RPS6KA2, IGHE/IGHG1/IGHD, DMAP1, SOCS3, and SETBP1– and an enhancer locus at chromosome 2 (2p25.1). The sites linked to TEX41, IGHE/IGHG1/IGHD, DMAP1, and SETBP1 were novel findings, while COX6A1P/FGD2, SBNO2, RPS6KA2, and SOCS3 had been identified previously with concordant direction of effects. RPS6KA2, DMAP1, and SETBP1 were replicated in the BMI-discordant monozygotic twin cohort using the FDR of 5%.Kirsti KvaløyChristian Magnus PageTurid Lingaas HolmenNature PortfolioarticleDifferentially Methylated SitesEpigenome-wide Association Studies (EWAS)Obesity DevelopmentTest Construction (TEX41)Obesity SusceptibilityMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Differentially Methylated Sites Epigenome-wide Association Studies (EWAS) Obesity Development Test Construction (TEX41) Obesity Susceptibility Medicine R Science Q
spellingShingle	Differentially Methylated Sites Epigenome-wide Association Studies (EWAS) Obesity Development Test Construction (TEX41) Obesity Susceptibility Medicine R Science Q Kirsti Kvaløy Christian Magnus Page Turid Lingaas Holmen Epigenome-wide methylation differences in a group of lean and obese women – A HUNT Study
description	Abstract Knowledge of epigenetically regulated biomarkers linked to obesity development is still scarce. Improving molecular understanding of the involved factors and pathways would improve obesity phenotype characterization and reveal potentially relevant targets for obesity intervention. The Illumina Infinium HumanMethylation450 BeadChip was used in a leucocyte epigenome-wide association study (EWAS) to quantify differential DNA methylation in 60 lean compared with 60 obese young women. Replication was done in monozygotic twins discordant for obesity. At adolescence and adulthood, the two weight groups differed significantly in obesity-related traits and metabolic risk factors. Differential hypomethylation was overrepresented in obese compared to lean women. In the adjusted model, the EWAS revealed 10 differentially methylated CpG sites linked to 8 gene loci – COX6A1P2/FGD2, SBNO2, TEX41, RPS6KA2, IGHE/IGHG1/IGHD, DMAP1, SOCS3, and SETBP1– and an enhancer locus at chromosome 2 (2p25.1). The sites linked to TEX41, IGHE/IGHG1/IGHD, DMAP1, and SETBP1 were novel findings, while COX6A1P/FGD2, SBNO2, RPS6KA2, and SOCS3 had been identified previously with concordant direction of effects. RPS6KA2, DMAP1, and SETBP1 were replicated in the BMI-discordant monozygotic twin cohort using the FDR of 5%.
format	article
author	Kirsti Kvaløy Christian Magnus Page Turid Lingaas Holmen
author_facet	Kirsti Kvaløy Christian Magnus Page Turid Lingaas Holmen
author_sort	Kirsti Kvaløy
title	Epigenome-wide methylation differences in a group of lean and obese women – A HUNT Study
title_short	Epigenome-wide methylation differences in a group of lean and obese women – A HUNT Study
title_full	Epigenome-wide methylation differences in a group of lean and obese women – A HUNT Study
title_fullStr	Epigenome-wide methylation differences in a group of lean and obese women – A HUNT Study
title_full_unstemmed	Epigenome-wide methylation differences in a group of lean and obese women – A HUNT Study
title_sort	epigenome-wide methylation differences in a group of lean and obese women – a hunt study
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/10b4271b851f45b8ad0dc658aa1e06e7
work_keys_str_mv	AT kirstikvaløy epigenomewidemethylationdifferencesinagroupofleanandobesewomenahuntstudy AT christianmagnuspage epigenomewidemethylationdifferencesinagroupofleanandobesewomenahuntstudy AT turidlingaasholmen epigenomewidemethylationdifferencesinagroupofleanandobesewomenahuntstudy
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Epigenome-wide methylation differences in a group of lean and obese women – A HUNT Study

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