Golgi membrane protein Erd1 Is essential for recycling a subset of Golgi glycosyltransferases

Protein glycosylation in the Golgi is a sequential process that requires proper distribution of transmembrane glycosyltransferase enzymes in the appropriate Golgi compartments. Some of the cytosolic machinery required for the steady-state localization of some Golgi enzymes are known but existing mod...

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Autores principales:	Richa Sardana, Carolyn M Highland, Beth E Straight, Christopher F Chavez, J Christopher Fromme, Scott D Emr
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	Golgi recycling Glycosylation Erd1 Vps74 glycosyl transferase COG complex Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/10bb80ac6b454dfda829d2f8791ad02b
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spelling	oai:doaj.org-article:10bb80ac6b454dfda829d2f8791ad02b2021-11-25T14:20:59ZGolgi membrane protein Erd1 Is essential for recycling a subset of Golgi glycosyltransferases10.7554/eLife.707742050-084Xe70774https://doaj.org/article/10bb80ac6b454dfda829d2f8791ad02b2021-11-01T00:00:00Zhttps://elifesciences.org/articles/70774https://doaj.org/toc/2050-084XProtein glycosylation in the Golgi is a sequential process that requires proper distribution of transmembrane glycosyltransferase enzymes in the appropriate Golgi compartments. Some of the cytosolic machinery required for the steady-state localization of some Golgi enzymes are known but existing models do not explain how many of these enzymes are localized. Here, we uncover the role of an integral membrane protein in yeast, Erd1, as a key facilitator of Golgi glycosyltransferase recycling by directly interacting with both the Golgi enzymes and the cytosolic receptor, Vps74. Loss of Erd1 function results in mislocalization of Golgi enzymes to the vacuole/lysosome. We present evidence that Erd1 forms an integral part of the recycling machinery and ensures productive recycling of several early Golgi enzymes. Our work provides new insights on how the localization of Golgi glycosyltransferases is spatially and temporally regulated, and is finely tuned to the cues of Golgi maturation.Richa SardanaCarolyn M HighlandBeth E StraightChristopher F ChavezJ Christopher FrommeScott D EmreLife Sciences Publications LtdarticleGolgi recyclingGlycosylationErd1Vps74glycosyl transferaseCOG complexMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Golgi recycling Glycosylation Erd1 Vps74 glycosyl transferase COG complex Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	Golgi recycling Glycosylation Erd1 Vps74 glycosyl transferase COG complex Medicine R Science Q Biology (General) QH301-705.5 Richa Sardana Carolyn M Highland Beth E Straight Christopher F Chavez J Christopher Fromme Scott D Emr Golgi membrane protein Erd1 Is essential for recycling a subset of Golgi glycosyltransferases
description	Protein glycosylation in the Golgi is a sequential process that requires proper distribution of transmembrane glycosyltransferase enzymes in the appropriate Golgi compartments. Some of the cytosolic machinery required for the steady-state localization of some Golgi enzymes are known but existing models do not explain how many of these enzymes are localized. Here, we uncover the role of an integral membrane protein in yeast, Erd1, as a key facilitator of Golgi glycosyltransferase recycling by directly interacting with both the Golgi enzymes and the cytosolic receptor, Vps74. Loss of Erd1 function results in mislocalization of Golgi enzymes to the vacuole/lysosome. We present evidence that Erd1 forms an integral part of the recycling machinery and ensures productive recycling of several early Golgi enzymes. Our work provides new insights on how the localization of Golgi glycosyltransferases is spatially and temporally regulated, and is finely tuned to the cues of Golgi maturation.
format	article
author	Richa Sardana Carolyn M Highland Beth E Straight Christopher F Chavez J Christopher Fromme Scott D Emr
author_facet	Richa Sardana Carolyn M Highland Beth E Straight Christopher F Chavez J Christopher Fromme Scott D Emr
author_sort	Richa Sardana
title	Golgi membrane protein Erd1 Is essential for recycling a subset of Golgi glycosyltransferases
title_short	Golgi membrane protein Erd1 Is essential for recycling a subset of Golgi glycosyltransferases
title_full	Golgi membrane protein Erd1 Is essential for recycling a subset of Golgi glycosyltransferases
title_fullStr	Golgi membrane protein Erd1 Is essential for recycling a subset of Golgi glycosyltransferases
title_full_unstemmed	Golgi membrane protein Erd1 Is essential for recycling a subset of Golgi glycosyltransferases
title_sort	golgi membrane protein erd1 is essential for recycling a subset of golgi glycosyltransferases
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/10bb80ac6b454dfda829d2f8791ad02b
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Golgi membrane protein Erd1 Is essential for recycling a subset of Golgi glycosyltransferases

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