Cascaded processing enables continuous upstream processing with E. coli BL21(DE3)

Abstract In many industrial sectors continuous processing is already the golden standard to maximize productivity. However, when working with living cells, subpopulation formation causes instabilities in long-term cultivations. In cascaded continuous cultivation, biomass formation and recombinant pr...

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Autores principales:	Stefan Kittler, Christoph Slouka, Andreas Pell, Roman Lamplot, Mihail Besleaga, Sarah Ablasser, Christoph Herwig, Oliver Spadiut, Julian Kopp
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/10bbc989405440259d5b4913a9470bc5
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spelling	oai:doaj.org-article:10bbc989405440259d5b4913a9470bc52021-12-02T18:25:04ZCascaded processing enables continuous upstream processing with E. coli BL21(DE3)10.1038/s41598-021-90899-92045-2322https://doaj.org/article/10bbc989405440259d5b4913a9470bc52021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90899-9https://doaj.org/toc/2045-2322Abstract In many industrial sectors continuous processing is already the golden standard to maximize productivity. However, when working with living cells, subpopulation formation causes instabilities in long-term cultivations. In cascaded continuous cultivation, biomass formation and recombinant protein expression can be spatially separated. This cultivation mode was found to facilitate stable protein expression using microbial hosts, however mechanistic knowledge of this cultivation strategy is scarce. In this contribution we present a method workflow to reduce workload and accelerate the establishment of stable continuous processes with E. coli BL21(DE3) exclusively based on bioengineering methods.Stefan KittlerChristoph SloukaAndreas PellRoman LamplotMihail BesleagaSarah AblasserChristoph HerwigOliver SpadiutJulian KoppNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefan Kittler Christoph Slouka Andreas Pell Roman Lamplot Mihail Besleaga Sarah Ablasser Christoph Herwig Oliver Spadiut Julian Kopp Cascaded processing enables continuous upstream processing with E. coli BL21(DE3)
description	Abstract In many industrial sectors continuous processing is already the golden standard to maximize productivity. However, when working with living cells, subpopulation formation causes instabilities in long-term cultivations. In cascaded continuous cultivation, biomass formation and recombinant protein expression can be spatially separated. This cultivation mode was found to facilitate stable protein expression using microbial hosts, however mechanistic knowledge of this cultivation strategy is scarce. In this contribution we present a method workflow to reduce workload and accelerate the establishment of stable continuous processes with E. coli BL21(DE3) exclusively based on bioengineering methods.
format	article
author	Stefan Kittler Christoph Slouka Andreas Pell Roman Lamplot Mihail Besleaga Sarah Ablasser Christoph Herwig Oliver Spadiut Julian Kopp
author_facet	Stefan Kittler Christoph Slouka Andreas Pell Roman Lamplot Mihail Besleaga Sarah Ablasser Christoph Herwig Oliver Spadiut Julian Kopp
author_sort	Stefan Kittler
title	Cascaded processing enables continuous upstream processing with E. coli BL21(DE3)
title_short	Cascaded processing enables continuous upstream processing with E. coli BL21(DE3)
title_full	Cascaded processing enables continuous upstream processing with E. coli BL21(DE3)
title_fullStr	Cascaded processing enables continuous upstream processing with E. coli BL21(DE3)
title_full_unstemmed	Cascaded processing enables continuous upstream processing with E. coli BL21(DE3)
title_sort	cascaded processing enables continuous upstream processing with e. coli bl21(de3)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/10bbc989405440259d5b4913a9470bc5
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Cascaded processing enables continuous upstream processing with E. coli BL21(DE3)

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