Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum

Abstract Background Paphiopedilum, commonly known as slipper orchid, is an important genus of orchid family with prominent horticultural value. Compared with conventional methods such as tillers and in vitro shoots multiplication, induction and regeneration of protocorm-like bodies (PLBs) is an effe...

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Autores principales:	Beiyi Guo, Songjun Zeng, Yuying Yin, Lin Li, Guohua Ma, Kunlin Wu, Lin Fang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Paphiopedilum Transcriptome analysis Protocorm-like bodies Somatic embryo Organogenesis Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/10c9d26a51474d368f264c85f31d9dd3
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spelling	oai:doaj.org-article:10c9d26a51474d368f264c85f31d9dd32021-11-14T12:26:45ZCharacterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum10.1186/s12864-021-08087-y1471-2164https://doaj.org/article/10c9d26a51474d368f264c85f31d9dd32021-11-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08087-yhttps://doaj.org/toc/1471-2164Abstract Background Paphiopedilum, commonly known as slipper orchid, is an important genus of orchid family with prominent horticultural value. Compared with conventional methods such as tillers and in vitro shoots multiplication, induction and regeneration of protocorm-like bodies (PLBs) is an effective micropropagation method in Paphiopedilum. The PLB initiation efficiency varies among species, hybrids and varieties, which leads to only a few Paphiopedilum species can be large-scale propagated through PLBs. So far, little is known about the mechanisms behind the initiation and maintenance of PLB in Paphiopedilum. Results A protocol to induce PLB development from seed-derived protocorms of Paphiopedilum SCBG Huihuang90 (P. SCBG Prince × P. SCBG Miracle) was established. The morphological characterization of four key PLB developmental stages showed that significant polarity and cell size gradients were observed within each PLB. The endogenous hormone level was evaluated. The increase in the levels of indoleacetic acid (IAA) and jasmonic acid (JA) accompanying the PLBs differentiation, suggesting auxin and JA levels were correlated with PLB development. Gibberellic acid (GA) decreased to a very low level, indicated that GA inactivation may be necessary for shoot apical meristem (SAM) development. Comparative transcriptomic profiles of four different developmental stages of P. SCBG Huihuang90 PLBs explore key genes involved in PLB development. The numbers of differentially expressed genes (DEGs) in three pairwise comparisons (A vs B, B vs C, C vs D) were 1455, 349, and 3529, respectively. KEGG enrichment analysis revealed that DEGs were implicated in secondary metabolite metabolism and photosynthesis. DEGs related to hormone metabolism and signaling, somatic embryogenesis, shoot development and photosynthesis were discussed in detail. Conclusion This study is the first report on PLB development in Paphiopedilum using transcriptome sequencing, which provides useful information to understand the mechanisms of PLB development.Beiyi GuoSongjun ZengYuying YinLin LiGuohua MaKunlin WuLin FangBMCarticlePaphiopedilumTranscriptome analysisProtocorm-like bodiesSomatic embryoOrganogenesisBiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Paphiopedilum Transcriptome analysis Protocorm-like bodies Somatic embryo Organogenesis Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Paphiopedilum Transcriptome analysis Protocorm-like bodies Somatic embryo Organogenesis Biotechnology TP248.13-248.65 Genetics QH426-470 Beiyi Guo Songjun Zeng Yuying Yin Lin Li Guohua Ma Kunlin Wu Lin Fang Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum
description	Abstract Background Paphiopedilum, commonly known as slipper orchid, is an important genus of orchid family with prominent horticultural value. Compared with conventional methods such as tillers and in vitro shoots multiplication, induction and regeneration of protocorm-like bodies (PLBs) is an effective micropropagation method in Paphiopedilum. The PLB initiation efficiency varies among species, hybrids and varieties, which leads to only a few Paphiopedilum species can be large-scale propagated through PLBs. So far, little is known about the mechanisms behind the initiation and maintenance of PLB in Paphiopedilum. Results A protocol to induce PLB development from seed-derived protocorms of Paphiopedilum SCBG Huihuang90 (P. SCBG Prince × P. SCBG Miracle) was established. The morphological characterization of four key PLB developmental stages showed that significant polarity and cell size gradients were observed within each PLB. The endogenous hormone level was evaluated. The increase in the levels of indoleacetic acid (IAA) and jasmonic acid (JA) accompanying the PLBs differentiation, suggesting auxin and JA levels were correlated with PLB development. Gibberellic acid (GA) decreased to a very low level, indicated that GA inactivation may be necessary for shoot apical meristem (SAM) development. Comparative transcriptomic profiles of four different developmental stages of P. SCBG Huihuang90 PLBs explore key genes involved in PLB development. The numbers of differentially expressed genes (DEGs) in three pairwise comparisons (A vs B, B vs C, C vs D) were 1455, 349, and 3529, respectively. KEGG enrichment analysis revealed that DEGs were implicated in secondary metabolite metabolism and photosynthesis. DEGs related to hormone metabolism and signaling, somatic embryogenesis, shoot development and photosynthesis were discussed in detail. Conclusion This study is the first report on PLB development in Paphiopedilum using transcriptome sequencing, which provides useful information to understand the mechanisms of PLB development.
format	article
author	Beiyi Guo Songjun Zeng Yuying Yin Lin Li Guohua Ma Kunlin Wu Lin Fang
author_facet	Beiyi Guo Songjun Zeng Yuying Yin Lin Li Guohua Ma Kunlin Wu Lin Fang
author_sort	Beiyi Guo
title	Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum
title_short	Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum
title_full	Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum
title_fullStr	Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum
title_full_unstemmed	Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum
title_sort	characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of paphiopedilum
publisher	BMC
publishDate	2021
url	https://doaj.org/article/10c9d26a51474d368f264c85f31d9dd3
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Characterization of phytohormone and transcriptome profiles during protocorm-like bodies development of Paphiopedilum

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