Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs

Animal hairs are an apt surface for retention of forensic trace epithelial samples. The aim of this study was threefold: to evaluate different methods of sample collection (moistened and dry swabs) and DNA extraction (Chelex® 100 method, Qiagen EZ1® DNA Investigator Kit), as well as to examine the m...

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Autores principales:	Špoljarić Branimira, Popović Maja, Crnjac Josip, Žderić Savatović Zrinka, Ratko Martina, Lozančić Mateja, Jurak Matea, Špoljarić Igor, Špoljarić Daniel, Mršić Gordan
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2016
Materias:	dna profiling sheep wool dog hair chelex® 100 qiagen ez1® dna investigator kit Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/10caddc2cc9448998bf7d6e2f080b04b
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id	oai:doaj.org-article:10caddc2cc9448998bf7d6e2f080b04b
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spelling	oai:doaj.org-article:10caddc2cc9448998bf7d6e2f080b04b2021-11-17T21:27:51ZGleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs1820-744810.1515/acve-2016-0016https://doaj.org/article/10caddc2cc9448998bf7d6e2f080b04b2016-06-01T00:00:00Zhttps://doi.org/10.1515/acve-2016-0016https://doaj.org/toc/1820-7448Animal hairs are an apt surface for retention of forensic trace epithelial samples. The aim of this study was threefold: to evaluate different methods of sample collection (moistened and dry swabs) and DNA extraction (Chelex® 100 method, Qiagen EZ1® DNA Investigator Kit), as well as to examine the morphological differences of hair fibres between two species (dog, sheep) and their ultimate impact on sample collection and processing. Our preliminary findings suggest that the use of EZ1® DNA Investigator Kit yields donor DNA profiles of higher quality. The results of different sample collection methods have shown intraspecific variations that require further investigation. The ability of retention and subsequent extraction of trace DNA appears to be similar between the two species, despite significant morphological differences between their coat hairs.Špoljarić BranimiraPopović MajaCrnjac JosipŽderić Savatović ZrinkaRatko MartinaLozančić MatejaJurak MateaŠpoljarić IgorŠpoljarić DanielMršić GordanSciendoarticledna profilingsheep wooldog hairchelex® 100qiagen ez1® dna investigator kitVeterinary medicineSF600-1100ENActa Veterinaria, Vol 66, Iss 2, Pp 187-202 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	dna profiling sheep wool dog hair chelex® 100 qiagen ez1® dna investigator kit Veterinary medicine SF600-1100
spellingShingle	dna profiling sheep wool dog hair chelex® 100 qiagen ez1® dna investigator kit Veterinary medicine SF600-1100 Špoljarić Branimira Popović Maja Crnjac Josip Žderić Savatović Zrinka Ratko Martina Lozančić Mateja Jurak Matea Špoljarić Igor Špoljarić Daniel Mršić Gordan Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs
description	Animal hairs are an apt surface for retention of forensic trace epithelial samples. The aim of this study was threefold: to evaluate different methods of sample collection (moistened and dry swabs) and DNA extraction (Chelex® 100 method, Qiagen EZ1® DNA Investigator Kit), as well as to examine the morphological differences of hair fibres between two species (dog, sheep) and their ultimate impact on sample collection and processing. Our preliminary findings suggest that the use of EZ1® DNA Investigator Kit yields donor DNA profiles of higher quality. The results of different sample collection methods have shown intraspecific variations that require further investigation. The ability of retention and subsequent extraction of trace DNA appears to be similar between the two species, despite significant morphological differences between their coat hairs.
format	article
author	Špoljarić Branimira Popović Maja Crnjac Josip Žderić Savatović Zrinka Ratko Martina Lozančić Mateja Jurak Matea Špoljarić Igor Špoljarić Daniel Mršić Gordan
author_facet	Špoljarić Branimira Popović Maja Crnjac Josip Žderić Savatović Zrinka Ratko Martina Lozančić Mateja Jurak Matea Špoljarić Igor Špoljarić Daniel Mršić Gordan
author_sort	Špoljarić Branimira
title	Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs
title_short	Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs
title_full	Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs
title_fullStr	Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs
title_full_unstemmed	Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs
title_sort	gleaning a human dna profile from trace swabs collected from animal hairs
publisher	Sciendo
publishDate	2016
url	https://doaj.org/article/10caddc2cc9448998bf7d6e2f080b04b
work_keys_str_mv	AT spoljaricbranimira gleaningahumandnaprofilefromtraceswabscollectedfromanimalhairs AT popovicmaja gleaningahumandnaprofilefromtraceswabscollectedfromanimalhairs AT crnjacjosip gleaningahumandnaprofilefromtraceswabscollectedfromanimalhairs AT zdericsavatoviczrinka gleaningahumandnaprofilefromtraceswabscollectedfromanimalhairs AT ratkomartina gleaningahumandnaprofilefromtraceswabscollectedfromanimalhairs AT lozancicmateja gleaningahumandnaprofilefromtraceswabscollectedfromanimalhairs AT jurakmatea gleaningahumandnaprofilefromtraceswabscollectedfromanimalhairs AT spoljaricigor gleaningahumandnaprofilefromtraceswabscollectedfromanimalhairs AT spoljaricdaniel gleaningahumandnaprofilefromtraceswabscollectedfromanimalhairs AT mrsicgordan gleaningahumandnaprofilefromtraceswabscollectedfromanimalhairs
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Gleaning a Human DNA Profile from Trace Swabs Collected from Animal Hairs

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