Mild mitochondrial uncoupling and calorie restriction increase fasting eNOS, akt and mitochondrial biogenesis.

Enhanced mitochondrial biogenesis promoted by eNOS activation is believed to play a central role in the beneficial effects of calorie restriction (CR). Since treatment of mice with dinitrophenol (DNP) promotes health and lifespan benefits similar to those observed in CR, we hypothesized that it coul...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Fernanda M Cerqueira, Francisco R M Laurindo, Alicia J Kowaltowski
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2011
Materias:
R
Q
Acceso en línea:https://doaj.org/article/10d992073ec84d959a6f5f3f207035c5
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:10d992073ec84d959a6f5f3f207035c5
record_format dspace
spelling oai:doaj.org-article:10d992073ec84d959a6f5f3f207035c52021-11-18T06:56:28ZMild mitochondrial uncoupling and calorie restriction increase fasting eNOS, akt and mitochondrial biogenesis.1932-620310.1371/journal.pone.0018433https://doaj.org/article/10d992073ec84d959a6f5f3f207035c52011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21483800/?tool=EBIhttps://doaj.org/toc/1932-6203Enhanced mitochondrial biogenesis promoted by eNOS activation is believed to play a central role in the beneficial effects of calorie restriction (CR). Since treatment of mice with dinitrophenol (DNP) promotes health and lifespan benefits similar to those observed in CR, we hypothesized that it could also impact biogenesis. We found that DNP and CR increase citrate synthase activity, PGC-1α, cytochrome c oxidase and mitofusin-2 expression, as well as fasting plasma levels of NO• products. In addition, eNOS and Akt phosphorylation in skeletal muscle and visceral adipose tissue was activated in fasting CR and DNP animals. Overall, our results indicate that systemic mild uncoupling activates eNOS and Akt-dependent pathways leading to mitochondrial biogenesis.Fernanda M CerqueiraFrancisco R M LaurindoAlicia J KowaltowskiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e18433 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Fernanda M Cerqueira
Francisco R M Laurindo
Alicia J Kowaltowski
Mild mitochondrial uncoupling and calorie restriction increase fasting eNOS, akt and mitochondrial biogenesis.
description Enhanced mitochondrial biogenesis promoted by eNOS activation is believed to play a central role in the beneficial effects of calorie restriction (CR). Since treatment of mice with dinitrophenol (DNP) promotes health and lifespan benefits similar to those observed in CR, we hypothesized that it could also impact biogenesis. We found that DNP and CR increase citrate synthase activity, PGC-1α, cytochrome c oxidase and mitofusin-2 expression, as well as fasting plasma levels of NO• products. In addition, eNOS and Akt phosphorylation in skeletal muscle and visceral adipose tissue was activated in fasting CR and DNP animals. Overall, our results indicate that systemic mild uncoupling activates eNOS and Akt-dependent pathways leading to mitochondrial biogenesis.
format article
author Fernanda M Cerqueira
Francisco R M Laurindo
Alicia J Kowaltowski
author_facet Fernanda M Cerqueira
Francisco R M Laurindo
Alicia J Kowaltowski
author_sort Fernanda M Cerqueira
title Mild mitochondrial uncoupling and calorie restriction increase fasting eNOS, akt and mitochondrial biogenesis.
title_short Mild mitochondrial uncoupling and calorie restriction increase fasting eNOS, akt and mitochondrial biogenesis.
title_full Mild mitochondrial uncoupling and calorie restriction increase fasting eNOS, akt and mitochondrial biogenesis.
title_fullStr Mild mitochondrial uncoupling and calorie restriction increase fasting eNOS, akt and mitochondrial biogenesis.
title_full_unstemmed Mild mitochondrial uncoupling and calorie restriction increase fasting eNOS, akt and mitochondrial biogenesis.
title_sort mild mitochondrial uncoupling and calorie restriction increase fasting enos, akt and mitochondrial biogenesis.
publisher Public Library of Science (PLoS)
publishDate 2011
url https://doaj.org/article/10d992073ec84d959a6f5f3f207035c5
work_keys_str_mv AT fernandamcerqueira mildmitochondrialuncouplingandcalorierestrictionincreasefastingenosaktandmitochondrialbiogenesis
AT franciscormlaurindo mildmitochondrialuncouplingandcalorierestrictionincreasefastingenosaktandmitochondrialbiogenesis
AT aliciajkowaltowski mildmitochondrialuncouplingandcalorierestrictionincreasefastingenosaktandmitochondrialbiogenesis
_version_ 1718424150041690112