A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages

Abstract The 3Rs principles (Replacement, Reduction and Refinement) are focused on finding alternatives to the use of animals in research. In this regard, cell lines are popular and useful tools for the replacement of primary cells in in vitro studies. However, around 15–30% of cell lines used in re...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Elizabeth Evans, Romain Paillot, María Rocío López-Álvarez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/10dc56861efd48eb9aaafd15ac182066
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:10dc56861efd48eb9aaafd15ac182066
record_format	dspace
spelling	oai:doaj.org-article:10dc56861efd48eb9aaafd15ac1820662021-12-02T15:07:50ZA comprehensive analysis of e-CAS cell line reveals they are mouse macrophages10.1038/s41598-018-26512-32045-2322https://doaj.org/article/10dc56861efd48eb9aaafd15ac1820662018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-26512-3https://doaj.org/toc/2045-2322Abstract The 3Rs principles (Replacement, Reduction and Refinement) are focused on finding alternatives to the use of animals in research. In this regard, cell lines are popular and useful tools for the replacement of primary cells in in vitro studies. However, around 15–30% of cell lines used in research have been misidentified or cross-contaminated generating concerns about the results obtained from experiments that use them. Here we described how old aliquots of an equine macrophage cell line (e-CAS) stored at the Animal Health Trust did not contain equine cells but macrophages of murine origin (m-CAS).Elizabeth EvansRomain PaillotMaría Rocío López-ÁlvarezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-6 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elizabeth Evans Romain Paillot María Rocío López-Álvarez A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages
description	Abstract The 3Rs principles (Replacement, Reduction and Refinement) are focused on finding alternatives to the use of animals in research. In this regard, cell lines are popular and useful tools for the replacement of primary cells in in vitro studies. However, around 15–30% of cell lines used in research have been misidentified or cross-contaminated generating concerns about the results obtained from experiments that use them. Here we described how old aliquots of an equine macrophage cell line (e-CAS) stored at the Animal Health Trust did not contain equine cells but macrophages of murine origin (m-CAS).
format	article
author	Elizabeth Evans Romain Paillot María Rocío López-Álvarez
author_facet	Elizabeth Evans Romain Paillot María Rocío López-Álvarez
author_sort	Elizabeth Evans
title	A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages
title_short	A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages
title_full	A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages
title_fullStr	A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages
title_full_unstemmed	A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages
title_sort	comprehensive analysis of e-cas cell line reveals they are mouse macrophages
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/10dc56861efd48eb9aaafd15ac182066
work_keys_str_mv	AT elizabethevans acomprehensiveanalysisofecascelllinerevealstheyaremousemacrophages AT romainpaillot acomprehensiveanalysisofecascelllinerevealstheyaremousemacrophages AT mariarociolopezalvarez acomprehensiveanalysisofecascelllinerevealstheyaremousemacrophages AT elizabethevans comprehensiveanalysisofecascelllinerevealstheyaremousemacrophages AT romainpaillot comprehensiveanalysisofecascelllinerevealstheyaremousemacrophages AT mariarociolopezalvarez comprehensiveanalysisofecascelllinerevealstheyaremousemacrophages
_version_	1718388366582480896

A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages

Ejemplares similares