Restricted differentiative capacity of Wt1-expressing peritoneal mesothelium in postnatal and adult mice

Abstract Previously, genetic lineage tracing based on the mesothelial marker Wt1, appeared to show that peritoneal mesothelial cells have a range of differentiative capacities and are the direct progenitors of vascular smooth muscle in the intestine. However, it was not clear whether this was a temp...

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Autores principales:	Thomas P. Wilm, Helen Tanton, Fiona Mutter, Veronica Foisor, Ben Middlehurst, Kelly Ward, Tarek Benameur, Nicholas Hastie, Bettina Wilm
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/10dd300136304e3780a651d8a7594163
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spelling	oai:doaj.org-article:10dd300136304e3780a651d8a75941632021-12-02T14:53:42ZRestricted differentiative capacity of Wt1-expressing peritoneal mesothelium in postnatal and adult mice10.1038/s41598-021-95380-12045-2322https://doaj.org/article/10dd300136304e3780a651d8a75941632021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95380-1https://doaj.org/toc/2045-2322Abstract Previously, genetic lineage tracing based on the mesothelial marker Wt1, appeared to show that peritoneal mesothelial cells have a range of differentiative capacities and are the direct progenitors of vascular smooth muscle in the intestine. However, it was not clear whether this was a temporally limited process or continued throughout postnatal life. Here, using a conditional Wt1-based genetic lineage tracing approach, we demonstrate that the postnatal and adult peritoneum covering intestine, mesentery and body wall only maintained itself and failed to contribute to other visceral tissues. Pulse-chase experiments of up to 6 months revealed that Wt1-expressing cells remained confined to the peritoneum and failed to differentiate into cellular components of blood vessels or other tissues underlying the peritoneum. Our data confirmed that the Wt1-lineage system also labelled submesothelial cells. Ablation of Wt1 in adult mice did not result in changes to the intestinal wall architecture. In the heart, we observed that Wt1-expressing cells maintained the epicardium and contributed to coronary vessels in newborn and adult mice. Our results demonstrate that Wt1-expressing cells in the peritoneum have limited differentiation capacities, and that contribution of Wt1-expressing cells to cardiac vasculature is based on organ-specific mechanisms.Thomas P. WilmHelen TantonFiona MutterVeronica FoisorBen MiddlehurstKelly WardTarek BenameurNicholas HastieBettina WilmNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thomas P. Wilm Helen Tanton Fiona Mutter Veronica Foisor Ben Middlehurst Kelly Ward Tarek Benameur Nicholas Hastie Bettina Wilm Restricted differentiative capacity of Wt1-expressing peritoneal mesothelium in postnatal and adult mice
description	Abstract Previously, genetic lineage tracing based on the mesothelial marker Wt1, appeared to show that peritoneal mesothelial cells have a range of differentiative capacities and are the direct progenitors of vascular smooth muscle in the intestine. However, it was not clear whether this was a temporally limited process or continued throughout postnatal life. Here, using a conditional Wt1-based genetic lineage tracing approach, we demonstrate that the postnatal and adult peritoneum covering intestine, mesentery and body wall only maintained itself and failed to contribute to other visceral tissues. Pulse-chase experiments of up to 6 months revealed that Wt1-expressing cells remained confined to the peritoneum and failed to differentiate into cellular components of blood vessels or other tissues underlying the peritoneum. Our data confirmed that the Wt1-lineage system also labelled submesothelial cells. Ablation of Wt1 in adult mice did not result in changes to the intestinal wall architecture. In the heart, we observed that Wt1-expressing cells maintained the epicardium and contributed to coronary vessels in newborn and adult mice. Our results demonstrate that Wt1-expressing cells in the peritoneum have limited differentiation capacities, and that contribution of Wt1-expressing cells to cardiac vasculature is based on organ-specific mechanisms.
format	article
author	Thomas P. Wilm Helen Tanton Fiona Mutter Veronica Foisor Ben Middlehurst Kelly Ward Tarek Benameur Nicholas Hastie Bettina Wilm
author_facet	Thomas P. Wilm Helen Tanton Fiona Mutter Veronica Foisor Ben Middlehurst Kelly Ward Tarek Benameur Nicholas Hastie Bettina Wilm
author_sort	Thomas P. Wilm
title	Restricted differentiative capacity of Wt1-expressing peritoneal mesothelium in postnatal and adult mice
title_short	Restricted differentiative capacity of Wt1-expressing peritoneal mesothelium in postnatal and adult mice
title_full	Restricted differentiative capacity of Wt1-expressing peritoneal mesothelium in postnatal and adult mice
title_fullStr	Restricted differentiative capacity of Wt1-expressing peritoneal mesothelium in postnatal and adult mice
title_full_unstemmed	Restricted differentiative capacity of Wt1-expressing peritoneal mesothelium in postnatal and adult mice
title_sort	restricted differentiative capacity of wt1-expressing peritoneal mesothelium in postnatal and adult mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/10dd300136304e3780a651d8a7594163
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Restricted differentiative capacity of Wt1-expressing peritoneal mesothelium in postnatal and adult mice

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