Sensitive detection and quantification of SARS-CoV-2 in saliva
Abstract Saliva has significant advantages as a test medium for detection of SARS-CoV-2 infection in patients, such as ease of collection, minimal requirement of supplies and trained personnel, and safety. Comprehensive validation in a large cohort of prospectively collected specimens with unknown S...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/10e5c7f5048c47d29112a27e324f9fe8 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:10e5c7f5048c47d29112a27e324f9fe8 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:10e5c7f5048c47d29112a27e324f9fe82021-12-02T17:40:47ZSensitive detection and quantification of SARS-CoV-2 in saliva10.1038/s41598-021-91835-72045-2322https://doaj.org/article/10e5c7f5048c47d29112a27e324f9fe82021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91835-7https://doaj.org/toc/2045-2322Abstract Saliva has significant advantages as a test medium for detection of SARS-CoV-2 infection in patients, such as ease of collection, minimal requirement of supplies and trained personnel, and safety. Comprehensive validation in a large cohort of prospectively collected specimens with unknown SARS-CoV-2 status should be performed to evaluate the potential and limitations of saliva-based testing. We developed a saliva-based testing pipeline for detection of SARS-CoV-2 nucleic acids using real-time reverse transcription PCR (RT-PCR) and droplet digital PCR (ddPCR) readouts, and measured samples from 137 outpatients tested at a curbside testing facility and 29 inpatients hospitalized for COVID-19. These measurements were compared to the nasal swab results for each patient performed by a certified microbiology laboratory. We found that our saliva testing positively detects 100% (RT-PCR) and 93.75% (ddPCR) of curbside patients that were identified as SARS-CoV-2 positive by the Emergency Use Authorization (EUA) certified nasal swab testing assay. Quantification of viral loads by ddPCR revealed an extremely wide range, with 1 million-fold difference between individual patients. Our results demonstrate for both community screening and hospital settings that saliva testing reliability is on par with that of the nasal swabs in detecting infected cases, and has potential for higher sensitivity when combined with ddPCR in detecting low-abundance viral loads that evade traditional testing methods.Mustafa Fatih AbasiyanikBlake FloodJing LinSefika OzcanSherin J. RouhaniAthalia PyzerJonathan TrujilloChaojie ZhenPing WuStephen JumicAndrew WangThomas F. GajewskiPeng WangMadeline HartleyBekim AmetiRachael NiemiecMarian FernandoVasudha MishraPeter SavageBulent AydoganCindy BethelScott MatushekKathleen G. BeavisNishant AgrawalJeremy SegalSavaş TayEvgeny IzumchenkoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Mustafa Fatih Abasiyanik Blake Flood Jing Lin Sefika Ozcan Sherin J. Rouhani Athalia Pyzer Jonathan Trujillo Chaojie Zhen Ping Wu Stephen Jumic Andrew Wang Thomas F. Gajewski Peng Wang Madeline Hartley Bekim Ameti Rachael Niemiec Marian Fernando Vasudha Mishra Peter Savage Bulent Aydogan Cindy Bethel Scott Matushek Kathleen G. Beavis Nishant Agrawal Jeremy Segal Savaş Tay Evgeny Izumchenko Sensitive detection and quantification of SARS-CoV-2 in saliva |
| description |
Abstract Saliva has significant advantages as a test medium for detection of SARS-CoV-2 infection in patients, such as ease of collection, minimal requirement of supplies and trained personnel, and safety. Comprehensive validation in a large cohort of prospectively collected specimens with unknown SARS-CoV-2 status should be performed to evaluate the potential and limitations of saliva-based testing. We developed a saliva-based testing pipeline for detection of SARS-CoV-2 nucleic acids using real-time reverse transcription PCR (RT-PCR) and droplet digital PCR (ddPCR) readouts, and measured samples from 137 outpatients tested at a curbside testing facility and 29 inpatients hospitalized for COVID-19. These measurements were compared to the nasal swab results for each patient performed by a certified microbiology laboratory. We found that our saliva testing positively detects 100% (RT-PCR) and 93.75% (ddPCR) of curbside patients that were identified as SARS-CoV-2 positive by the Emergency Use Authorization (EUA) certified nasal swab testing assay. Quantification of viral loads by ddPCR revealed an extremely wide range, with 1 million-fold difference between individual patients. Our results demonstrate for both community screening and hospital settings that saliva testing reliability is on par with that of the nasal swabs in detecting infected cases, and has potential for higher sensitivity when combined with ddPCR in detecting low-abundance viral loads that evade traditional testing methods. |
| format |
article |
| author |
Mustafa Fatih Abasiyanik Blake Flood Jing Lin Sefika Ozcan Sherin J. Rouhani Athalia Pyzer Jonathan Trujillo Chaojie Zhen Ping Wu Stephen Jumic Andrew Wang Thomas F. Gajewski Peng Wang Madeline Hartley Bekim Ameti Rachael Niemiec Marian Fernando Vasudha Mishra Peter Savage Bulent Aydogan Cindy Bethel Scott Matushek Kathleen G. Beavis Nishant Agrawal Jeremy Segal Savaş Tay Evgeny Izumchenko |
| author_facet |
Mustafa Fatih Abasiyanik Blake Flood Jing Lin Sefika Ozcan Sherin J. Rouhani Athalia Pyzer Jonathan Trujillo Chaojie Zhen Ping Wu Stephen Jumic Andrew Wang Thomas F. Gajewski Peng Wang Madeline Hartley Bekim Ameti Rachael Niemiec Marian Fernando Vasudha Mishra Peter Savage Bulent Aydogan Cindy Bethel Scott Matushek Kathleen G. Beavis Nishant Agrawal Jeremy Segal Savaş Tay Evgeny Izumchenko |
| author_sort |
Mustafa Fatih Abasiyanik |
| title |
Sensitive detection and quantification of SARS-CoV-2 in saliva |
| title_short |
Sensitive detection and quantification of SARS-CoV-2 in saliva |
| title_full |
Sensitive detection and quantification of SARS-CoV-2 in saliva |
| title_fullStr |
Sensitive detection and quantification of SARS-CoV-2 in saliva |
| title_full_unstemmed |
Sensitive detection and quantification of SARS-CoV-2 in saliva |
| title_sort |
sensitive detection and quantification of sars-cov-2 in saliva |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/10e5c7f5048c47d29112a27e324f9fe8 |
| work_keys_str_mv |
AT mustafafatihabasiyanik sensitivedetectionandquantificationofsarscov2insaliva AT blakeflood sensitivedetectionandquantificationofsarscov2insaliva AT jinglin sensitivedetectionandquantificationofsarscov2insaliva AT sefikaozcan sensitivedetectionandquantificationofsarscov2insaliva AT sherinjrouhani sensitivedetectionandquantificationofsarscov2insaliva AT athaliapyzer sensitivedetectionandquantificationofsarscov2insaliva AT jonathantrujillo sensitivedetectionandquantificationofsarscov2insaliva AT chaojiezhen sensitivedetectionandquantificationofsarscov2insaliva AT pingwu sensitivedetectionandquantificationofsarscov2insaliva AT stephenjumic sensitivedetectionandquantificationofsarscov2insaliva AT andrewwang sensitivedetectionandquantificationofsarscov2insaliva AT thomasfgajewski sensitivedetectionandquantificationofsarscov2insaliva AT pengwang sensitivedetectionandquantificationofsarscov2insaliva AT madelinehartley sensitivedetectionandquantificationofsarscov2insaliva AT bekimameti sensitivedetectionandquantificationofsarscov2insaliva AT rachaelniemiec sensitivedetectionandquantificationofsarscov2insaliva AT marianfernando sensitivedetectionandquantificationofsarscov2insaliva AT vasudhamishra sensitivedetectionandquantificationofsarscov2insaliva AT petersavage sensitivedetectionandquantificationofsarscov2insaliva AT bulentaydogan sensitivedetectionandquantificationofsarscov2insaliva AT cindybethel sensitivedetectionandquantificationofsarscov2insaliva AT scottmatushek sensitivedetectionandquantificationofsarscov2insaliva AT kathleengbeavis sensitivedetectionandquantificationofsarscov2insaliva AT nishantagrawal sensitivedetectionandquantificationofsarscov2insaliva AT jeremysegal sensitivedetectionandquantificationofsarscov2insaliva AT savastay sensitivedetectionandquantificationofsarscov2insaliva AT evgenyizumchenko sensitivedetectionandquantificationofsarscov2insaliva |
| _version_ |
1718379747733405696 |