Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1

Abstract Poly(thionine)-Au, a novel multifunctional substrate with excellent redox signal, enzyme-like activity, and easy antibody immobilisation, was synthesised using HAuCl4 as the oxidising agent and thionine as the monomer. The prepared poly(thionine)-Au composite exhibited an admirable electroc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Huiqiang Wang, Xin Gao, Zhanfang Ma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/10e7bfef4e0f4379a9e588095cfd6848
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:10e7bfef4e0f4379a9e588095cfd6848
record_format	dspace
spelling	oai:doaj.org-article:10e7bfef4e0f4379a9e588095cfd68482021-12-02T15:05:32ZMultifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-110.1038/s41598-017-01250-02045-2322https://doaj.org/article/10e7bfef4e0f4379a9e588095cfd68482017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01250-0https://doaj.org/toc/2045-2322Abstract Poly(thionine)-Au, a novel multifunctional substrate with excellent redox signal, enzyme-like activity, and easy antibody immobilisation, was synthesised using HAuCl4 as the oxidising agent and thionine as the monomer. The prepared poly(thionine)-Au composite exhibited an admirable electrochemical redox signal at −0.15 V and excellent H2O2 catalytic ability. In addition, gold nanoparticles in this composite were found to directly immobilise antibodies and further improve conductivity. In addition, a label-free electrochemical immunosensor was developed using poly(thionine)-Au as the sensing substrate for ultrasensitive detection of cytokeratin antigen 21-1 (CYFRA 21-1), an immunoassay found in human serum. The prepared immunosensor showed a wide liner range from 100 ng mL−1 to 10 fg mL−1 and an ultralow detection limit of 4.6 fg mL−1 (the ratio of signal to noise (S/N) = 3). Additionally, this method was used to analyse human serum samples and yielded results consistency with those of ELISA, implying its potential application in clinical research. The poly(thionine)-Au composite can be easily extended to other polymer-based nanocomposites, which is significant for other electrochemical immunoassays.Huiqiang WangXin GaoZhanfang MaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Huiqiang Wang Xin Gao Zhanfang Ma Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1
description	Abstract Poly(thionine)-Au, a novel multifunctional substrate with excellent redox signal, enzyme-like activity, and easy antibody immobilisation, was synthesised using HAuCl4 as the oxidising agent and thionine as the monomer. The prepared poly(thionine)-Au composite exhibited an admirable electrochemical redox signal at −0.15 V and excellent H2O2 catalytic ability. In addition, gold nanoparticles in this composite were found to directly immobilise antibodies and further improve conductivity. In addition, a label-free electrochemical immunosensor was developed using poly(thionine)-Au as the sensing substrate for ultrasensitive detection of cytokeratin antigen 21-1 (CYFRA 21-1), an immunoassay found in human serum. The prepared immunosensor showed a wide liner range from 100 ng mL−1 to 10 fg mL−1 and an ultralow detection limit of 4.6 fg mL−1 (the ratio of signal to noise (S/N) = 3). Additionally, this method was used to analyse human serum samples and yielded results consistency with those of ELISA, implying its potential application in clinical research. The poly(thionine)-Au composite can be easily extended to other polymer-based nanocomposites, which is significant for other electrochemical immunoassays.
format	article
author	Huiqiang Wang Xin Gao Zhanfang Ma
author_facet	Huiqiang Wang Xin Gao Zhanfang Ma
author_sort	Huiqiang Wang
title	Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1
title_short	Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1
title_full	Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1
title_fullStr	Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1
title_full_unstemmed	Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1
title_sort	multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/10e7bfef4e0f4379a9e588095cfd6848
work_keys_str_mv	AT huiqiangwang multifunctionalsubstrateoflabelfreeelectrochemicalimmunosensorforultrasensitivedetectionofcytokeratinsantigen211 AT xingao multifunctionalsubstrateoflabelfreeelectrochemicalimmunosensorforultrasensitivedetectionofcytokeratinsantigen211 AT zhanfangma multifunctionalsubstrateoflabelfreeelectrochemicalimmunosensorforultrasensitivedetectionofcytokeratinsantigen211
_version_	1718388749172211712

Multifunctional substrate of label-free electrochemical immunosensor for ultrasensitive detection of cytokeratins antigen 21-1

Ejemplares similares