Control of Arabidopsis shoot stem cell homeostasis by two antagonistic CLE peptide signalling pathways

Stem cell homeostasis in plant shoot meristems requires tight coordination between stem cell proliferation and cell differentiation. In Arabidopsis, stem cells express the secreted dodecapeptide CLAVATA3 (CLV3), which signals through the leucine-rich repeat (LRR)-receptor kinase CLAVATA1 (CLV1) and...

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Autores principales:	Jenia Schlegel, Gregoire Denay, Rene Wink, Karine Gustavo Pinto, Yvonne Stahl, Julia Schmid, Patrick Blümke, Rüdiger GW Simon
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	shoot meristem stem cells CLE40 CLAVATA signalling WUS Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/10f0ee34285e4c0b881ce5e44e015330
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spelling	oai:doaj.org-article:10f0ee34285e4c0b881ce5e44e0153302021-11-16T17:33:42ZControl of Arabidopsis shoot stem cell homeostasis by two antagonistic CLE peptide signalling pathways10.7554/eLife.709342050-084Xe70934https://doaj.org/article/10f0ee34285e4c0b881ce5e44e0153302021-10-01T00:00:00Zhttps://elifesciences.org/articles/70934https://doaj.org/toc/2050-084XStem cell homeostasis in plant shoot meristems requires tight coordination between stem cell proliferation and cell differentiation. In Arabidopsis, stem cells express the secreted dodecapeptide CLAVATA3 (CLV3), which signals through the leucine-rich repeat (LRR)-receptor kinase CLAVATA1 (CLV1) and related CLV1-family members to downregulate expression of the homeodomain transcription factor WUSCHEL (WUS). WUS protein moves from cells below the stem cell domain to the meristem tip and promotes stem cell identity, together with CLV3 expression, generating a negative feedback loop. How stem cell activity in the meristem centre is coordinated with organ initiation and cell differentiation at the periphery is unknown. We show here that the CLE40 gene, encoding a secreted peptide closely related to CLV3, is expressed in the SAM in differentiating cells in a pattern complementary to that of CLV3. CLE40 promotes WUS expression via BAM1, a CLV1-family receptor, and CLE40 expression is in turn repressed in a WUS-dependent manner. Together, CLE40-BAM1-WUS establish a second negative feedback loop. We propose that stem cell homeostasis is achieved through two intertwined pathways that adjust WUS activity and incorporate information on the size of the stem cell domain, via CLV3-CLV1, and on cell differentiation via CLE40-BAM1.Jenia SchlegelGregoire DenayRene WinkKarine Gustavo PintoYvonne StahlJulia SchmidPatrick BlümkeRüdiger GW SimoneLife Sciences Publications Ltdarticleshoot meristemstem cellsCLE40CLAVATAsignallingWUSMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	shoot meristem stem cells CLE40 CLAVATA signalling WUS Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	shoot meristem stem cells CLE40 CLAVATA signalling WUS Medicine R Science Q Biology (General) QH301-705.5 Jenia Schlegel Gregoire Denay Rene Wink Karine Gustavo Pinto Yvonne Stahl Julia Schmid Patrick Blümke Rüdiger GW Simon Control of Arabidopsis shoot stem cell homeostasis by two antagonistic CLE peptide signalling pathways
description	Stem cell homeostasis in plant shoot meristems requires tight coordination between stem cell proliferation and cell differentiation. In Arabidopsis, stem cells express the secreted dodecapeptide CLAVATA3 (CLV3), which signals through the leucine-rich repeat (LRR)-receptor kinase CLAVATA1 (CLV1) and related CLV1-family members to downregulate expression of the homeodomain transcription factor WUSCHEL (WUS). WUS protein moves from cells below the stem cell domain to the meristem tip and promotes stem cell identity, together with CLV3 expression, generating a negative feedback loop. How stem cell activity in the meristem centre is coordinated with organ initiation and cell differentiation at the periphery is unknown. We show here that the CLE40 gene, encoding a secreted peptide closely related to CLV3, is expressed in the SAM in differentiating cells in a pattern complementary to that of CLV3. CLE40 promotes WUS expression via BAM1, a CLV1-family receptor, and CLE40 expression is in turn repressed in a WUS-dependent manner. Together, CLE40-BAM1-WUS establish a second negative feedback loop. We propose that stem cell homeostasis is achieved through two intertwined pathways that adjust WUS activity and incorporate information on the size of the stem cell domain, via CLV3-CLV1, and on cell differentiation via CLE40-BAM1.
format	article
author	Jenia Schlegel Gregoire Denay Rene Wink Karine Gustavo Pinto Yvonne Stahl Julia Schmid Patrick Blümke Rüdiger GW Simon
author_facet	Jenia Schlegel Gregoire Denay Rene Wink Karine Gustavo Pinto Yvonne Stahl Julia Schmid Patrick Blümke Rüdiger GW Simon
author_sort	Jenia Schlegel
title	Control of Arabidopsis shoot stem cell homeostasis by two antagonistic CLE peptide signalling pathways
title_short	Control of Arabidopsis shoot stem cell homeostasis by two antagonistic CLE peptide signalling pathways
title_full	Control of Arabidopsis shoot stem cell homeostasis by two antagonistic CLE peptide signalling pathways
title_fullStr	Control of Arabidopsis shoot stem cell homeostasis by two antagonistic CLE peptide signalling pathways
title_full_unstemmed	Control of Arabidopsis shoot stem cell homeostasis by two antagonistic CLE peptide signalling pathways
title_sort	control of arabidopsis shoot stem cell homeostasis by two antagonistic cle peptide signalling pathways
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/10f0ee34285e4c0b881ce5e44e015330
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_version_	1718426283606540288

Control of Arabidopsis shoot stem cell homeostasis by two antagonistic CLE peptide signalling pathways

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