A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant <i>Xerophyta</i> <i>schlechteri</i> during Dehydration

Vegetative desiccation tolerance, or the ability to survive the loss of ~95% relative water content (RWC), is rare in angiosperms, with these being commonly called resurrection plants. It is a complex multigenic and multi-factorial trait, with its understanding requiring a comprehensive systems biol...

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Autores principales:	Hawwa Gabier, David L. Tabb, Jill M. Farrant, Mohamed Suhail Rafudeen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	dehydration desiccation tolerance plant proteomics label-free quantification metabolomics resurrection plant Science Q
Acceso en línea:	https://doaj.org/article/10f6ff511cf04c04a4505fbadf7b76ac
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spelling	oai:doaj.org-article:10f6ff511cf04c04a4505fbadf7b76ac2021-11-25T18:11:28ZA Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant <i>Xerophyta</i> <i>schlechteri</i> during Dehydration10.3390/life111112422075-1729https://doaj.org/article/10f6ff511cf04c04a4505fbadf7b76ac2021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1242https://doaj.org/toc/2075-1729Vegetative desiccation tolerance, or the ability to survive the loss of ~95% relative water content (RWC), is rare in angiosperms, with these being commonly called resurrection plants. It is a complex multigenic and multi-factorial trait, with its understanding requiring a comprehensive systems biology approach. The aim of the current study was to conduct a label-free proteomic analysis of leaves of the resurrection plant <i>Xerophyta schlechteri</i> in response to desiccation. A targeted metabolomics approach was validated and correlated to the proteomics, contributing the missing link in studies on this species. Three physiological stages were identified: an early response to drying, during which the leaf tissues declined from full turgor to a RWC of ~80–70%, a mid-response in which the RWC declined to 40% and a late response where the tissues declined to 10% RWC. We identified 517 distinct proteins that were differentially expressed, of which 253 proteins were upregulated and 264 were downregulated in response to the three drying stages. Metabolomics analyses, which included monitoring the levels of a selection of phytohormones, amino acids, sugars, sugar alcohols, fatty acids and organic acids in response to dehydration, correlated with some of the proteomic differences, giving insight into the biological processes apparently involved in desiccation tolerance in this species.Hawwa GabierDavid L. TabbJill M. FarrantMohamed Suhail RafudeenMDPI AGarticledehydrationdesiccation toleranceplant proteomicslabel-free quantificationmetabolomicsresurrection plantScienceQENLife, Vol 11, Iss 1242, p 1242 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dehydration desiccation tolerance plant proteomics label-free quantification metabolomics resurrection plant Science Q
spellingShingle	dehydration desiccation tolerance plant proteomics label-free quantification metabolomics resurrection plant Science Q Hawwa Gabier David L. Tabb Jill M. Farrant Mohamed Suhail Rafudeen A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant <i>Xerophyta</i> <i>schlechteri</i> during Dehydration
description	Vegetative desiccation tolerance, or the ability to survive the loss of ~95% relative water content (RWC), is rare in angiosperms, with these being commonly called resurrection plants. It is a complex multigenic and multi-factorial trait, with its understanding requiring a comprehensive systems biology approach. The aim of the current study was to conduct a label-free proteomic analysis of leaves of the resurrection plant <i>Xerophyta schlechteri</i> in response to desiccation. A targeted metabolomics approach was validated and correlated to the proteomics, contributing the missing link in studies on this species. Three physiological stages were identified: an early response to drying, during which the leaf tissues declined from full turgor to a RWC of ~80–70%, a mid-response in which the RWC declined to 40% and a late response where the tissues declined to 10% RWC. We identified 517 distinct proteins that were differentially expressed, of which 253 proteins were upregulated and 264 were downregulated in response to the three drying stages. Metabolomics analyses, which included monitoring the levels of a selection of phytohormones, amino acids, sugars, sugar alcohols, fatty acids and organic acids in response to dehydration, correlated with some of the proteomic differences, giving insight into the biological processes apparently involved in desiccation tolerance in this species.
format	article
author	Hawwa Gabier David L. Tabb Jill M. Farrant Mohamed Suhail Rafudeen
author_facet	Hawwa Gabier David L. Tabb Jill M. Farrant Mohamed Suhail Rafudeen
author_sort	Hawwa Gabier
title	A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant <i>Xerophyta</i> <i>schlechteri</i> during Dehydration
title_short	A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant <i>Xerophyta</i> <i>schlechteri</i> during Dehydration
title_full	A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant <i>Xerophyta</i> <i>schlechteri</i> during Dehydration
title_fullStr	A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant <i>Xerophyta</i> <i>schlechteri</i> during Dehydration
title_full_unstemmed	A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant <i>Xerophyta</i> <i>schlechteri</i> during Dehydration
title_sort	label-free proteomic and complementary metabolomic analysis of leaves of the resurrection plant <i>xerophyta</i> <i>schlechteri</i> during dehydration
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/10f6ff511cf04c04a4505fbadf7b76ac
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A Label-Free Proteomic and Complementary Metabolomic Analysis of Leaves of the Resurrection Plant <i>Xerophyta</i> <i>schlechteri</i> during Dehydration

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