Novel <named-content content-type="genus-species">Xanthomonas campestris</named-content> Long-Chain-Specific 3-Oxoacyl-Acyl Carrier Protein Reductase Involved in Diffusible Signal Factor Synthesis

ABSTRACT The precursors of the diffusible signal factor (DSF) family signals of Xanthomonas campestris pv. campestris are 3-hydroxyacyl-acyl carrier protein (3-hydroxyacyl-ACP) thioesters having acyl chains of 12 to 13 carbon atoms produced by the fatty acid biosynthetic pathway. We report a novel 3...

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Autores principales:	Zhe Hu, Huijuan Dong, Jin-Cheng Ma, Yonghong Yu, Kai-Hui Li, Qiao-Qiao Guo, Chao Zhang, Wen-Bin Zhang, Xinyun Cao, John E. Cronan, Haihong Wang
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Xanthomonas fatty acids quorum sensing Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/10fd44b34fac497097a72dcacbc6126c
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spelling	oai:doaj.org-article:10fd44b34fac497097a72dcacbc6126c2021-11-15T16:00:24ZNovel <named-content content-type="genus-species">Xanthomonas campestris</named-content> Long-Chain-Specific 3-Oxoacyl-Acyl Carrier Protein Reductase Involved in Diffusible Signal Factor Synthesis10.1128/mBio.00596-182150-7511https://doaj.org/article/10fd44b34fac497097a72dcacbc6126c2018-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00596-18https://doaj.org/toc/2150-7511ABSTRACT The precursors of the diffusible signal factor (DSF) family signals of Xanthomonas campestris pv. campestris are 3-hydroxyacyl-acyl carrier protein (3-hydroxyacyl-ACP) thioesters having acyl chains of 12 to 13 carbon atoms produced by the fatty acid biosynthetic pathway. We report a novel 3-oxoacyl-ACP reductase encoded by the X. campestris pv. campestris XCC0416 gene (fabG2), which is unable to participate in the initial steps of fatty acyl synthesis. This was shown by the failure of FabG2 expression to allow growth at the nonpermissive temperature of an Escherichia coli fabG temperature-sensitive strain. However, when transformed into the E. coli strain together with a plasmid bearing the Vibrio harveyi acyl-ACP synthetase gene (aasS), growth proceeded, but only when the medium contained octanoic acid. In vitro assays showed that FabG2 catalyzes the reduction of long-chain (≥C8) 3-oxoacyl-ACPs to 3-hydroxyacyl-ACPs but is only weakly active with shorter-chain (C4, C6) substrates. FabG1, the housekeeping 3-oxoacyl-ACP reductase encoded within the fatty acid synthesis gene cluster, could be deleted in a strain that overexpressed fabG2 but only in octanoic acid-supplemented media. Growth of the X. campestris pv. campestris ΔfabG1 strain overexpressing fabG2 required fabH for growth with octanoic acid, indicating that octanoyl coenzyme A is elongated by X. campestris pv. campestris fabH. Deletion of fabG2 reduced DSF family signal production, whereas overproduction of either FabG1 or FabG2 in the ΔfabG2 strain restored DSF family signal levels. IMPORTANCE Quorum sensing mediated by DSF signaling molecules regulates pathogenesis in several different phytopathogenic bacteria, including Xanthomonas campestris pv. campestris. DSF signaling also plays a key role in infection by the human pathogen Burkholderia cepacia. The acyl chains of the DSF molecules are diverted and remodeled from a key intermediate of the fatty acid synthesis pathway. We report a Xanthomonas campestris pv. campestris fatty acid synthesis enzyme, FabG2, of novel specificity that seems tailored to provide DSF signaling molecule precursors.Zhe HuHuijuan DongJin-Cheng MaYonghong YuKai-Hui LiQiao-Qiao GuoChao ZhangWen-Bin ZhangXinyun CaoJohn E. CronanHaihong WangAmerican Society for MicrobiologyarticleXanthomonasfatty acidsquorum sensingMicrobiologyQR1-502ENmBio, Vol 9, Iss 3 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Xanthomonas fatty acids quorum sensing Microbiology QR1-502
spellingShingle	Xanthomonas fatty acids quorum sensing Microbiology QR1-502 Zhe Hu Huijuan Dong Jin-Cheng Ma Yonghong Yu Kai-Hui Li Qiao-Qiao Guo Chao Zhang Wen-Bin Zhang Xinyun Cao John E. Cronan Haihong Wang Novel <named-content content-type="genus-species">Xanthomonas campestris</named-content> Long-Chain-Specific 3-Oxoacyl-Acyl Carrier Protein Reductase Involved in Diffusible Signal Factor Synthesis
description	ABSTRACT The precursors of the diffusible signal factor (DSF) family signals of Xanthomonas campestris pv. campestris are 3-hydroxyacyl-acyl carrier protein (3-hydroxyacyl-ACP) thioesters having acyl chains of 12 to 13 carbon atoms produced by the fatty acid biosynthetic pathway. We report a novel 3-oxoacyl-ACP reductase encoded by the X. campestris pv. campestris XCC0416 gene (fabG2), which is unable to participate in the initial steps of fatty acyl synthesis. This was shown by the failure of FabG2 expression to allow growth at the nonpermissive temperature of an Escherichia coli fabG temperature-sensitive strain. However, when transformed into the E. coli strain together with a plasmid bearing the Vibrio harveyi acyl-ACP synthetase gene (aasS), growth proceeded, but only when the medium contained octanoic acid. In vitro assays showed that FabG2 catalyzes the reduction of long-chain (≥C8) 3-oxoacyl-ACPs to 3-hydroxyacyl-ACPs but is only weakly active with shorter-chain (C4, C6) substrates. FabG1, the housekeeping 3-oxoacyl-ACP reductase encoded within the fatty acid synthesis gene cluster, could be deleted in a strain that overexpressed fabG2 but only in octanoic acid-supplemented media. Growth of the X. campestris pv. campestris ΔfabG1 strain overexpressing fabG2 required fabH for growth with octanoic acid, indicating that octanoyl coenzyme A is elongated by X. campestris pv. campestris fabH. Deletion of fabG2 reduced DSF family signal production, whereas overproduction of either FabG1 or FabG2 in the ΔfabG2 strain restored DSF family signal levels. IMPORTANCE Quorum sensing mediated by DSF signaling molecules regulates pathogenesis in several different phytopathogenic bacteria, including Xanthomonas campestris pv. campestris. DSF signaling also plays a key role in infection by the human pathogen Burkholderia cepacia. The acyl chains of the DSF molecules are diverted and remodeled from a key intermediate of the fatty acid synthesis pathway. We report a Xanthomonas campestris pv. campestris fatty acid synthesis enzyme, FabG2, of novel specificity that seems tailored to provide DSF signaling molecule precursors.
format	article
author	Zhe Hu Huijuan Dong Jin-Cheng Ma Yonghong Yu Kai-Hui Li Qiao-Qiao Guo Chao Zhang Wen-Bin Zhang Xinyun Cao John E. Cronan Haihong Wang
author_facet	Zhe Hu Huijuan Dong Jin-Cheng Ma Yonghong Yu Kai-Hui Li Qiao-Qiao Guo Chao Zhang Wen-Bin Zhang Xinyun Cao John E. Cronan Haihong Wang
author_sort	Zhe Hu
title	Novel <named-content content-type="genus-species">Xanthomonas campestris</named-content> Long-Chain-Specific 3-Oxoacyl-Acyl Carrier Protein Reductase Involved in Diffusible Signal Factor Synthesis
title_short	Novel <named-content content-type="genus-species">Xanthomonas campestris</named-content> Long-Chain-Specific 3-Oxoacyl-Acyl Carrier Protein Reductase Involved in Diffusible Signal Factor Synthesis
title_full	Novel <named-content content-type="genus-species">Xanthomonas campestris</named-content> Long-Chain-Specific 3-Oxoacyl-Acyl Carrier Protein Reductase Involved in Diffusible Signal Factor Synthesis
title_fullStr	Novel <named-content content-type="genus-species">Xanthomonas campestris</named-content> Long-Chain-Specific 3-Oxoacyl-Acyl Carrier Protein Reductase Involved in Diffusible Signal Factor Synthesis
title_full_unstemmed	Novel <named-content content-type="genus-species">Xanthomonas campestris</named-content> Long-Chain-Specific 3-Oxoacyl-Acyl Carrier Protein Reductase Involved in Diffusible Signal Factor Synthesis
title_sort	novel <named-content content-type="genus-species">xanthomonas campestris</named-content> long-chain-specific 3-oxoacyl-acyl carrier protein reductase involved in diffusible signal factor synthesis
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/10fd44b34fac497097a72dcacbc6126c
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Novel <named-content content-type="genus-species">Xanthomonas campestris</named-content> Long-Chain-Specific 3-Oxoacyl-Acyl Carrier Protein Reductase Involved in Diffusible Signal Factor Synthesis

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