Optogenetic delay of status epilepticus onset in an in vivo rodent epilepsy model.

Epilepsy is a devastating disease, currently treated with medications, surgery or electrical stimulation. None of these approaches is totally effective and our ability to control seizures remains limited and complicated by frequent side effects. The emerging revolutionary technique of optogenetics e...

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Autores principales:	Inna Sukhotinsky, Alexander M Chan, Omar J Ahmed, Vikram R Rao, Viviana Gradinaru, Charu Ramakrishnan, Karl Deisseroth, Ania K Majewska, Sydney S Cash
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1102cb4939e94baebeb2eb38613deae7
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spelling	oai:doaj.org-article:1102cb4939e94baebeb2eb38613deae72021-11-18T07:48:00ZOptogenetic delay of status epilepticus onset in an in vivo rodent epilepsy model.1932-620310.1371/journal.pone.0062013https://doaj.org/article/1102cb4939e94baebeb2eb38613deae72013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23637949/?tool=EBIhttps://doaj.org/toc/1932-6203Epilepsy is a devastating disease, currently treated with medications, surgery or electrical stimulation. None of these approaches is totally effective and our ability to control seizures remains limited and complicated by frequent side effects. The emerging revolutionary technique of optogenetics enables manipulation of the activity of specific neuronal populations in vivo with exquisite spatiotemporal resolution using light. We used optogenetic approaches to test the role of hippocampal excitatory neurons in the lithium-pilocarpine model of acute elicited seizures in awake behaving rats. Hippocampal pyramidal neurons were transduced in vivo with a virus carrying an enhanced halorhodopsin (eNpHR), a yellow light activated chloride pump, and acute seizure progression was then monitored behaviorally and electrophysiologically in the presence and absence of illumination delivered via an optical fiber. Inhibition of those neurons with illumination prior to seizure onset significantly delayed electrographic and behavioral initiation of status epilepticus, and altered the dynamics of ictal activity development. These results reveal an essential role of hippocampal excitatory neurons in this model of ictogenesis and illustrate the power of optogenetic approaches for elucidation of seizure mechanisms. This early success in controlling seizures also suggests future therapeutic avenues.Inna SukhotinskyAlexander M ChanOmar J AhmedVikram R RaoViviana GradinaruCharu RamakrishnanKarl DeisserothAnia K MajewskaSydney S CashPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e62013 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Inna Sukhotinsky Alexander M Chan Omar J Ahmed Vikram R Rao Viviana Gradinaru Charu Ramakrishnan Karl Deisseroth Ania K Majewska Sydney S Cash Optogenetic delay of status epilepticus onset in an in vivo rodent epilepsy model.
description	Epilepsy is a devastating disease, currently treated with medications, surgery or electrical stimulation. None of these approaches is totally effective and our ability to control seizures remains limited and complicated by frequent side effects. The emerging revolutionary technique of optogenetics enables manipulation of the activity of specific neuronal populations in vivo with exquisite spatiotemporal resolution using light. We used optogenetic approaches to test the role of hippocampal excitatory neurons in the lithium-pilocarpine model of acute elicited seizures in awake behaving rats. Hippocampal pyramidal neurons were transduced in vivo with a virus carrying an enhanced halorhodopsin (eNpHR), a yellow light activated chloride pump, and acute seizure progression was then monitored behaviorally and electrophysiologically in the presence and absence of illumination delivered via an optical fiber. Inhibition of those neurons with illumination prior to seizure onset significantly delayed electrographic and behavioral initiation of status epilepticus, and altered the dynamics of ictal activity development. These results reveal an essential role of hippocampal excitatory neurons in this model of ictogenesis and illustrate the power of optogenetic approaches for elucidation of seizure mechanisms. This early success in controlling seizures also suggests future therapeutic avenues.
format	article
author	Inna Sukhotinsky Alexander M Chan Omar J Ahmed Vikram R Rao Viviana Gradinaru Charu Ramakrishnan Karl Deisseroth Ania K Majewska Sydney S Cash
author_facet	Inna Sukhotinsky Alexander M Chan Omar J Ahmed Vikram R Rao Viviana Gradinaru Charu Ramakrishnan Karl Deisseroth Ania K Majewska Sydney S Cash
author_sort	Inna Sukhotinsky
title	Optogenetic delay of status epilepticus onset in an in vivo rodent epilepsy model.
title_short	Optogenetic delay of status epilepticus onset in an in vivo rodent epilepsy model.
title_full	Optogenetic delay of status epilepticus onset in an in vivo rodent epilepsy model.
title_fullStr	Optogenetic delay of status epilepticus onset in an in vivo rodent epilepsy model.
title_full_unstemmed	Optogenetic delay of status epilepticus onset in an in vivo rodent epilepsy model.
title_sort	optogenetic delay of status epilepticus onset in an in vivo rodent epilepsy model.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/1102cb4939e94baebeb2eb38613deae7
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Optogenetic delay of status epilepticus onset in an in vivo rodent epilepsy model.

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