Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles

Single-cell genomics can be used to study uncultured microorganisms. Here, Stepanauskas et al. present a method combining improved multiple displacement amplification and FACS, to obtain genomic sequences and cell size information from uncultivated microbial cells and viral particles in environmenta...

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Autores principales:	Ramunas Stepanauskas, Elizabeth A. Fergusson, Joseph Brown, Nicole J. Poulton, Ben Tupper, Jessica M. Labonté, Eric D. Becraft, Julia M. Brown, Maria G. Pachiadaki, Tadas Povilaitis, Brian P. Thompson, Corianna J. Mascena, Wendy K. Bellows, Arvydas Lubys
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/1103091ab4b04fd8bf75b654668bcb21
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spelling	oai:doaj.org-article:1103091ab4b04fd8bf75b654668bcb212021-12-02T10:48:10ZImproved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles10.1038/s41467-017-00128-z2041-1723https://doaj.org/article/1103091ab4b04fd8bf75b654668bcb212017-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-00128-zhttps://doaj.org/toc/2041-1723Single-cell genomics can be used to study uncultured microorganisms. Here, Stepanauskas et al. present a method combining improved multiple displacement amplification and FACS, to obtain genomic sequences and cell size information from uncultivated microbial cells and viral particles in environmental samples.Ramunas StepanauskasElizabeth A. FergussonJoseph BrownNicole J. PoultonBen TupperJessica M. LabontéEric D. BecraftJulia M. BrownMaria G. PachiadakiTadas PovilaitisBrian P. ThompsonCorianna J. MascenaWendy K. BellowsArvydas LubysNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Ramunas Stepanauskas Elizabeth A. Fergusson Joseph Brown Nicole J. Poulton Ben Tupper Jessica M. Labonté Eric D. Becraft Julia M. Brown Maria G. Pachiadaki Tadas Povilaitis Brian P. Thompson Corianna J. Mascena Wendy K. Bellows Arvydas Lubys Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles
description	Single-cell genomics can be used to study uncultured microorganisms. Here, Stepanauskas et al. present a method combining improved multiple displacement amplification and FACS, to obtain genomic sequences and cell size information from uncultivated microbial cells and viral particles in environmental samples.
format	article
author	Ramunas Stepanauskas Elizabeth A. Fergusson Joseph Brown Nicole J. Poulton Ben Tupper Jessica M. Labonté Eric D. Becraft Julia M. Brown Maria G. Pachiadaki Tadas Povilaitis Brian P. Thompson Corianna J. Mascena Wendy K. Bellows Arvydas Lubys
author_facet	Ramunas Stepanauskas Elizabeth A. Fergusson Joseph Brown Nicole J. Poulton Ben Tupper Jessica M. Labonté Eric D. Becraft Julia M. Brown Maria G. Pachiadaki Tadas Povilaitis Brian P. Thompson Corianna J. Mascena Wendy K. Bellows Arvydas Lubys
author_sort	Ramunas Stepanauskas
title	Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles
title_short	Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles
title_full	Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles
title_fullStr	Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles
title_full_unstemmed	Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles
title_sort	improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1103091ab4b04fd8bf75b654668bcb21
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Improved genome recovery and integrated cell-size analyses of individual uncultured microbial cells and viral particles

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