Characterization of an enantioselective odorant receptor in the yellow fever mosquito Aedes aegypti.

Enantiomers differ only in the left or right handedness (chirality) of their orientations and exhibit identical chemical and physical properties. In chemical communication systems, enantiomers can be differentially active at the physiological and behavioral levels. Only recently were enantioselectiv...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jonathan D Bohbot, Joseph C Dickens
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/11038f2e264f4fe3bdda1798b8e1d312
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:11038f2e264f4fe3bdda1798b8e1d312
record_format	dspace
spelling	oai:doaj.org-article:11038f2e264f4fe3bdda1798b8e1d3122021-11-25T06:20:23ZCharacterization of an enantioselective odorant receptor in the yellow fever mosquito Aedes aegypti.1932-620310.1371/journal.pone.0007032https://doaj.org/article/11038f2e264f4fe3bdda1798b8e1d3122009-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19753115/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Enantiomers differ only in the left or right handedness (chirality) of their orientations and exhibit identical chemical and physical properties. In chemical communication systems, enantiomers can be differentially active at the physiological and behavioral levels. Only recently were enantioselective odorant receptors demonstrated in mammals while their existence in insects has remained hypothetical. Using the two-microelectrode voltage clamp of Xenopus oocytes, we show that the yellow fever mosquito, Aedes aegypti, odorant receptor 8 (AaOR8) acts as a chiral selective receptor for the (R)-(-)-enantiomer of 1-octen-3-ol, which in the presence of other kairomones is an attractant used by blood-sucking insects to locate their hosts. In addition to steric constraints, chain length and degree of unsaturation play important roles in this recognition process. This is the first characterization of an enantioselective odorant receptor in insects and the results demonstrate that an OR alone, without helper proteins, can account for chiral specificity exhibited by olfactory sensory neurons (OSNs).Jonathan D BohbotJoseph C DickensPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 9, p e7032 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jonathan D Bohbot Joseph C Dickens Characterization of an enantioselective odorant receptor in the yellow fever mosquito Aedes aegypti.
description	Enantiomers differ only in the left or right handedness (chirality) of their orientations and exhibit identical chemical and physical properties. In chemical communication systems, enantiomers can be differentially active at the physiological and behavioral levels. Only recently were enantioselective odorant receptors demonstrated in mammals while their existence in insects has remained hypothetical. Using the two-microelectrode voltage clamp of Xenopus oocytes, we show that the yellow fever mosquito, Aedes aegypti, odorant receptor 8 (AaOR8) acts as a chiral selective receptor for the (R)-(-)-enantiomer of 1-octen-3-ol, which in the presence of other kairomones is an attractant used by blood-sucking insects to locate their hosts. In addition to steric constraints, chain length and degree of unsaturation play important roles in this recognition process. This is the first characterization of an enantioselective odorant receptor in insects and the results demonstrate that an OR alone, without helper proteins, can account for chiral specificity exhibited by olfactory sensory neurons (OSNs).
format	article
author	Jonathan D Bohbot Joseph C Dickens
author_facet	Jonathan D Bohbot Joseph C Dickens
author_sort	Jonathan D Bohbot
title	Characterization of an enantioselective odorant receptor in the yellow fever mosquito Aedes aegypti.
title_short	Characterization of an enantioselective odorant receptor in the yellow fever mosquito Aedes aegypti.
title_full	Characterization of an enantioselective odorant receptor in the yellow fever mosquito Aedes aegypti.
title_fullStr	Characterization of an enantioselective odorant receptor in the yellow fever mosquito Aedes aegypti.
title_full_unstemmed	Characterization of an enantioselective odorant receptor in the yellow fever mosquito Aedes aegypti.
title_sort	characterization of an enantioselective odorant receptor in the yellow fever mosquito aedes aegypti.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/11038f2e264f4fe3bdda1798b8e1d312
work_keys_str_mv	AT jonathandbohbot characterizationofanenantioselectiveodorantreceptorintheyellowfevermosquitoaedesaegypti AT josephcdickens characterizationofanenantioselectiveodorantreceptorintheyellowfevermosquitoaedesaegypti
_version_	1718413786621149184

Characterization of an enantioselective odorant receptor in the yellow fever mosquito Aedes aegypti.

Ejemplares similares