Gut Microbiota Contributes to Host Defense Against Klebsiella pneumoniae-Induced Liver Abscess

Gut Microbiota Contributes to Host Defense Against Klebsiella pneumoniae-Induced Liver Abscess

Yahong Zheng,1 Yuting Ding,1 Mengran Xu,1 Haoran Chen,1 Hui zhang,1 Yanyan Liu,1– 3 Weihua Shen,4 Jiabin Li1– 3,5 1Department of Infectious Diseases, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People’s Republic of China; 2Anhui Center for Surveillance of Bacterial Resis...

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Autores principales: Zheng Y, Ding Y, Xu M, Chen H, zhang H, Liu Y, Shen W, Li J
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2021
Materias:
klebsiella pneumoniae
liver abscess
gut microbiome
fecal microbiota transplantation
inflammation
Pathology
RB1-214
Therapeutics. Pharmacology
RM1-950
Acceso en línea:https://doaj.org/article/1104f30fda864191b23ba2734357316d
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spelling oai:doaj.org-article:1104f30fda864191b23ba2734357316d2021-12-02T18:01:51ZGut Microbiota Contributes to Host Defense Against Klebsiella pneumoniae-Induced Liver Abscess1178-7031https://doaj.org/article/1104f30fda864191b23ba2734357316d2021-10-01T00:00:00Zhttps://www.dovepress.com/gut-microbiota-contributes-to-host-defense-against-klebsiella-pneumoni-peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Yahong Zheng,1 Yuting Ding,1 Mengran Xu,1 Haoran Chen,1 Hui zhang,1 Yanyan Liu,1– 3 Weihua Shen,4 Jiabin Li1– 3,5 1Department of Infectious Diseases, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People’s Republic of China; 2Anhui Center for Surveillance of Bacterial Resistance, Hefei, Anhui, People’s Republic of China; 3Institute of Bacterial Resistance, Anhui Medical University, Hefei, Anhui, People’s Republic of China; 4Department of Special Clinic, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People’s Republic of China; 5Department of Infectious Diseases, The Chaohu Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People’s Republic of ChinaCorrespondence: Jiabin LiDepartment of Infectious Diseases, The First Affiliated Hospital and the Chaohu Hospital of Anhui Medical University, Jixi road 218, Hefei, Anhui, 230022, People’s Republic of ChinaEmail lijiabin@ahmu.edu.cnWeihua ShenDepartment of Special Clinic, The First Affiliated Hospital of Anhui Medical University, Jixi road 218, Hefei, Anhui, 230022, People’s Republic of ChinaEmail Shenweihua2021810@163.comPurpose: Klebsiella pneumoniae-induced liver abscess (KLA) is a type of pyogenic liver abscess (PLA), which is a distinct invasive syndrome that has been increasingly reported worldwide over the past two decades. The intestinal microbiota is increasingly recognized as an important modulator that can promote and maintain host immune homeostasis. However, its precise role in liver abscess is unknown. We aimed to investigate the function of the gut microbiota in the host defense against K. pneumoniae infection.Methods: We constructed C57BL/6J mice with KLA and analyzed the diversity and richness of the intestinal microflora by 16S rRNA sequencing. Next, to create a microbiota-depleted (MD) mouse model, we administered multiple broad-spectrum antibiotics and validated the model using 16S rRNA sequencing. At 48 h after K. pneumoniae infection, we assessed the general health condition, liver injury, bacterial loads, and inflammatory factor levels in MD+KLA mice. Additionally, fecal microbiota transplantation (FMT) was conducted in another group of MD+KLA mice prior to K. pneumoniae infection, and we assessed whether the transplantation changed the outcomes.Results: The diversity of the intestinal flora was significantly changed in KLA mice compared to control mice, with a decrease in beneficial bacteria and an increase in harmful bacteria. The MD+KLA mice exhibited impaired antimicrobial capacity, reduced survival, increased inflammation and liver damage at 48 h after K. pneumoniae infection compared to the KLA mice. However, FMT normalized the inflammatory cytokine levels, reduced liver damage, and increased survival.Conclusion: This study identified the gut microbiota as a protective factor against K. pneumoniae infection. The role of FMT in KLA should be investigated in future clinical studies.Keywords: Klebsiella pneumoniae, liver abscess, gut microbiome, fecal microbiota transplantation, inflammationZheng YDing YXu MChen Hzhang HLiu YShen WLi JDove Medical Pressarticleklebsiella pneumoniaeliver abscessgut microbiomefecal microbiota transplantationinflammationPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 5215-5225 (2021)
institution DOAJ
collection DOAJ
language EN
topic klebsiella pneumoniae
liver abscess
gut microbiome
fecal microbiota transplantation
inflammation
Pathology
RB1-214
Therapeutics. Pharmacology
RM1-950
spellingShingle klebsiella pneumoniae
liver abscess
gut microbiome
fecal microbiota transplantation
inflammation
Pathology
RB1-214
Therapeutics. Pharmacology
RM1-950
Zheng Y
Ding Y
Xu M
Chen H
zhang H
Liu Y
Shen W
Li J
Gut Microbiota Contributes to Host Defense Against Klebsiella pneumoniae-Induced Liver Abscess
description Yahong Zheng,1 Yuting Ding,1 Mengran Xu,1 Haoran Chen,1 Hui zhang,1 Yanyan Liu,1– 3 Weihua Shen,4 Jiabin Li1– 3,5 1Department of Infectious Diseases, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People’s Republic of China; 2Anhui Center for Surveillance of Bacterial Resistance, Hefei, Anhui, People’s Republic of China; 3Institute of Bacterial Resistance, Anhui Medical University, Hefei, Anhui, People’s Republic of China; 4Department of Special Clinic, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People’s Republic of China; 5Department of Infectious Diseases, The Chaohu Affiliated Hospital of Anhui Medical University, Hefei, Anhui, People’s Republic of ChinaCorrespondence: Jiabin LiDepartment of Infectious Diseases, The First Affiliated Hospital and the Chaohu Hospital of Anhui Medical University, Jixi road 218, Hefei, Anhui, 230022, People’s Republic of ChinaEmail lijiabin@ahmu.edu.cnWeihua ShenDepartment of Special Clinic, The First Affiliated Hospital of Anhui Medical University, Jixi road 218, Hefei, Anhui, 230022, People’s Republic of ChinaEmail Shenweihua2021810@163.comPurpose: Klebsiella pneumoniae-induced liver abscess (KLA) is a type of pyogenic liver abscess (PLA), which is a distinct invasive syndrome that has been increasingly reported worldwide over the past two decades. The intestinal microbiota is increasingly recognized as an important modulator that can promote and maintain host immune homeostasis. However, its precise role in liver abscess is unknown. We aimed to investigate the function of the gut microbiota in the host defense against K. pneumoniae infection.Methods: We constructed C57BL/6J mice with KLA and analyzed the diversity and richness of the intestinal microflora by 16S rRNA sequencing. Next, to create a microbiota-depleted (MD) mouse model, we administered multiple broad-spectrum antibiotics and validated the model using 16S rRNA sequencing. At 48 h after K. pneumoniae infection, we assessed the general health condition, liver injury, bacterial loads, and inflammatory factor levels in MD+KLA mice. Additionally, fecal microbiota transplantation (FMT) was conducted in another group of MD+KLA mice prior to K. pneumoniae infection, and we assessed whether the transplantation changed the outcomes.Results: The diversity of the intestinal flora was significantly changed in KLA mice compared to control mice, with a decrease in beneficial bacteria and an increase in harmful bacteria. The MD+KLA mice exhibited impaired antimicrobial capacity, reduced survival, increased inflammation and liver damage at 48 h after K. pneumoniae infection compared to the KLA mice. However, FMT normalized the inflammatory cytokine levels, reduced liver damage, and increased survival.Conclusion: This study identified the gut microbiota as a protective factor against K. pneumoniae infection. The role of FMT in KLA should be investigated in future clinical studies.Keywords: Klebsiella pneumoniae, liver abscess, gut microbiome, fecal microbiota transplantation, inflammation
format article
author Zheng Y
Ding Y
Xu M
Chen H
zhang H
Liu Y
Shen W
Li J
author_facet Zheng Y
Ding Y
Xu M
Chen H
zhang H
Liu Y
Shen W
Li J
author_sort Zheng Y
title Gut Microbiota Contributes to Host Defense Against Klebsiella pneumoniae-Induced Liver Abscess
title_short Gut Microbiota Contributes to Host Defense Against Klebsiella pneumoniae-Induced Liver Abscess
title_full Gut Microbiota Contributes to Host Defense Against Klebsiella pneumoniae-Induced Liver Abscess
title_fullStr Gut Microbiota Contributes to Host Defense Against Klebsiella pneumoniae-Induced Liver Abscess
title_full_unstemmed Gut Microbiota Contributes to Host Defense Against Klebsiella pneumoniae-Induced Liver Abscess
title_sort gut microbiota contributes to host defense against klebsiella pneumoniae-induced liver abscess
publisher Dove Medical Press
publishDate 2021
url https://doaj.org/article/1104f30fda864191b23ba2734357316d
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