Exposure of Metal Oxide Nanoparticles on the Bioluminescence Process of <i>P<sub>u</sub>-</i> and <i>P<sub>m</sub>-lux</i> Recombinant <i>P. putida mt-2</i> Strains
Comparison of the effects of metal oxide nanoparticles (NPs; CuO, NiO, ZnO, TiO<sub>2</sub>, and Al<sub>2</sub>O<sub>3</sub>) on different bioluminescence processes was evaluated using two recombinant (<i>P<sub>m</sub>-lux</i> and <i>...
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| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/111294334e974f9e87c1ff60a804aa0a |
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| Sumario: | Comparison of the effects of metal oxide nanoparticles (NPs; CuO, NiO, ZnO, TiO<sub>2</sub>, and Al<sub>2</sub>O<sub>3</sub>) on different bioluminescence processes was evaluated using two recombinant (<i>P<sub>m</sub>-lux</i> and <i>P<sub>u</sub>-lux</i>) strains of <i>Pseudomonas putida mt-2</i> with same inducer exposure. Different sensitivities and responses were observed according to the type of NPs and recombinant strains. EC<sub>50</sub> values were determined. The negative effects on the bioluminescence activity of the <i>P<sub>m</sub>-lux</i> strain was greater than for the <i>P<sub>u</sub>-lux</i> strains for all NPs tested. EC<sub>50</sub> values for the <i>P<sub>m</sub>-lux</i> strain were 1.7- to 6.2-fold lower (corresponding to high inhibition) than for <i>P<sub>u</sub>-lux</i>. ZnO NP caused the greatest inhibition among the tested NPs in both strains, showing approximately 11 times less EC<sub>50s</sub> of CuO, which appeared as the least inhibited. Although NPs showed different sensitivities depending on the bioluminescence process, similar orders of EC<sub>50s</sub> for both strains were observed as follows: ZnO > NiO, Al<sub>2</sub>O<sub>3</sub> > TiO<sub>2</sub> > CuO. More detailed in-depth systematic approaches, including in the field of molecular mechanisms, is needed to evaluate the accurate effect mechanisms involved in both bioluminescence metabolic processes. |
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