Exposure of Metal Oxide Nanoparticles on the Bioluminescence Process of Pu- and Pm-lux Recombinant P. putida mt-2 Strains

Comparison of the effects of metal oxide nanoparticles (NPs; CuO, NiO, ZnO, TiO2, and Al2O3) on different bioluminescence processes was evaluated using two recombinant (Pm-lux and ...

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Autores principales:	In Chul Kong, Kyung-Seok Ko, Sohyeon Lee, Dong-Chan Koh, Robert Burlage
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bioluminescence inducer nanoparticles <i>P<sub>m</sub>-</i> and <i>P<sub>u</sub>-lux</i> recombinant Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/111294334e974f9e87c1ff60a804aa0a
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spelling	oai:doaj.org-article:111294334e974f9e87c1ff60a804aa0a2021-11-25T18:30:05ZExposure of Metal Oxide Nanoparticles on the Bioluminescence Process of <i>P<sub>u</sub>-</i> and <i>P<sub>m</sub>-lux</i> Recombinant <i>P. putida mt-2</i> Strains10.3390/nano111128222079-4991https://doaj.org/article/111294334e974f9e87c1ff60a804aa0a2021-10-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/2822https://doaj.org/toc/2079-4991Comparison of the effects of metal oxide nanoparticles (NPs; CuO, NiO, ZnO, TiO<sub>2</sub>, and Al<sub>2</sub>O<sub>3</sub>) on different bioluminescence processes was evaluated using two recombinant (<i>P<sub>m</sub>-lux</i> and <i>P<sub>u</sub>-lux</i>) strains of <i>Pseudomonas putida mt-2</i> with same inducer exposure. Different sensitivities and responses were observed according to the type of NPs and recombinant strains. EC<sub>50</sub> values were determined. The negative effects on the bioluminescence activity of the <i>P<sub>m</sub>-lux</i> strain was greater than for the <i>P<sub>u</sub>-lux</i> strains for all NPs tested. EC<sub>50</sub> values for the <i>P<sub>m</sub>-lux</i> strain were 1.7- to 6.2-fold lower (corresponding to high inhibition) than for <i>P<sub>u</sub>-lux</i>. ZnO NP caused the greatest inhibition among the tested NPs in both strains, showing approximately 11 times less EC<sub>50s</sub> of CuO, which appeared as the least inhibited. Although NPs showed different sensitivities depending on the bioluminescence process, similar orders of EC<sub>50s</sub> for both strains were observed as follows: ZnO > NiO, Al<sub>2</sub>O<sub>3</sub> > TiO<sub>2</sub> > CuO. More detailed in-depth systematic approaches, including in the field of molecular mechanisms, is needed to evaluate the accurate effect mechanisms involved in both bioluminescence metabolic processes.In Chul KongKyung-Seok KoSohyeon LeeDong-Chan KohRobert BurlageMDPI AGarticlebioluminescenceinducernanoparticles<i>P<sub>m</sub>-</i> and <i>P<sub>u</sub>-lux</i>recombinantChemistryQD1-999ENNanomaterials, Vol 11, Iss 2822, p 2822 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bioluminescence inducer nanoparticles <i>P<sub>m</sub>-</i> and <i>P<sub>u</sub>-lux</i> recombinant Chemistry QD1-999
spellingShingle	bioluminescence inducer nanoparticles <i>P<sub>m</sub>-</i> and <i>P<sub>u</sub>-lux</i> recombinant Chemistry QD1-999 In Chul Kong Kyung-Seok Ko Sohyeon Lee Dong-Chan Koh Robert Burlage Exposure of Metal Oxide Nanoparticles on the Bioluminescence Process of <i>P<sub>u</sub>-</i> and <i>P<sub>m</sub>-lux</i> Recombinant <i>P. putida mt-2</i> Strains
description	Comparison of the effects of metal oxide nanoparticles (NPs; CuO, NiO, ZnO, TiO<sub>2</sub>, and Al<sub>2</sub>O<sub>3</sub>) on different bioluminescence processes was evaluated using two recombinant (<i>P<sub>m</sub>-lux</i> and <i>P<sub>u</sub>-lux</i>) strains of <i>Pseudomonas putida mt-2</i> with same inducer exposure. Different sensitivities and responses were observed according to the type of NPs and recombinant strains. EC<sub>50</sub> values were determined. The negative effects on the bioluminescence activity of the <i>P<sub>m</sub>-lux</i> strain was greater than for the <i>P<sub>u</sub>-lux</i> strains for all NPs tested. EC<sub>50</sub> values for the <i>P<sub>m</sub>-lux</i> strain were 1.7- to 6.2-fold lower (corresponding to high inhibition) than for <i>P<sub>u</sub>-lux</i>. ZnO NP caused the greatest inhibition among the tested NPs in both strains, showing approximately 11 times less EC<sub>50s</sub> of CuO, which appeared as the least inhibited. Although NPs showed different sensitivities depending on the bioluminescence process, similar orders of EC<sub>50s</sub> for both strains were observed as follows: ZnO > NiO, Al<sub>2</sub>O<sub>3</sub> > TiO<sub>2</sub> > CuO. More detailed in-depth systematic approaches, including in the field of molecular mechanisms, is needed to evaluate the accurate effect mechanisms involved in both bioluminescence metabolic processes.
format	article
author	In Chul Kong Kyung-Seok Ko Sohyeon Lee Dong-Chan Koh Robert Burlage
author_facet	In Chul Kong Kyung-Seok Ko Sohyeon Lee Dong-Chan Koh Robert Burlage
author_sort	In Chul Kong
title	Exposure of Metal Oxide Nanoparticles on the Bioluminescence Process of <i>P<sub>u</sub>-</i> and <i>P<sub>m</sub>-lux</i> Recombinant <i>P. putida mt-2</i> Strains
title_short	Exposure of Metal Oxide Nanoparticles on the Bioluminescence Process of <i>P<sub>u</sub>-</i> and <i>P<sub>m</sub>-lux</i> Recombinant <i>P. putida mt-2</i> Strains
title_full	Exposure of Metal Oxide Nanoparticles on the Bioluminescence Process of <i>P<sub>u</sub>-</i> and <i>P<sub>m</sub>-lux</i> Recombinant <i>P. putida mt-2</i> Strains
title_fullStr	Exposure of Metal Oxide Nanoparticles on the Bioluminescence Process of <i>P<sub>u</sub>-</i> and <i>P<sub>m</sub>-lux</i> Recombinant <i>P. putida mt-2</i> Strains
title_full_unstemmed	Exposure of Metal Oxide Nanoparticles on the Bioluminescence Process of <i>P<sub>u</sub>-</i> and <i>P<sub>m</sub>-lux</i> Recombinant <i>P. putida mt-2</i> Strains
title_sort	exposure of metal oxide nanoparticles on the bioluminescence process of <i>p<sub>u</sub>-</i> and <i>p<sub>m</sub>-lux</i> recombinant <i>p. putida mt-2</i> strains
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/111294334e974f9e87c1ff60a804aa0a
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_version_	1718411058911117312

Exposure of Metal Oxide Nanoparticles on the Bioluminescence Process of <i>P<sub>u</sub>-</i> and <i>P<sub>m</sub>-lux</i> Recombinant <i>P. putida mt-2</i> Strains

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