Role of cytokines in pathogenesis, diagnosis and efficiency evaluation of immunotherapy in various variants of sclerotic lichen in women

Our aim was to evaluate diagnostic and pathogenetic significance of plasma cytokines (IL-20, IL-23, IL-10, TNFα, IFNγ) in the patients with various clinical and histological variants of sclerotic lichen and to assess opportunity for their use as effectiveness criteria of immunotherapy for this disea...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	E. V. Kolesnikova, A. V. Zharov, G. A. Penzhoyan
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2021
Materias:	benign vulvar diseases sclerotic lichen cytokines immunotherapy preparations of nucleic acids clinical and immunological efficiency optimization of diagnosis optimization of treatment Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/1114502e29764a598b4fdecbd65fafa5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1114502e29764a598b4fdecbd65fafa5
record_format	dspace
spelling	oai:doaj.org-article:1114502e29764a598b4fdecbd65fafa52021-11-18T08:03:50ZRole of cytokines in pathogenesis, diagnosis and efficiency evaluation of immunotherapy in various variants of sclerotic lichen in women1563-06252313-741X10.15789/1563-0625-ROC-2085https://doaj.org/article/1114502e29764a598b4fdecbd65fafa52021-03-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/2085https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XOur aim was to evaluate diagnostic and pathogenetic significance of plasma cytokines (IL-20, IL-23, IL-10, TNFα, IFNγ) in the patients with various clinical and histological variants of sclerotic lichen and to assess opportunity for their use as effectiveness criteria of immunotherapy for this disease using a drug based on eukaryotic deoxyribonucleic acid (Derinat). The prospective cohort study included assessment of the clinical manifestations (itching and dyspareunia) and measurement of blood cytokine contents (IL-20, IL-23, IL-10, TNFα, IFNγ) in women (n = 114) with various clinical variants of sclerotic lichen (atrophic, sclerotic and sclerotic-atrophic) before and after immunotherapy with a nucleic acid-based drug (Derinat). Derinat was chosen due to the fact of being an agonist of Toll-like receptors, and a number of immunoregulatory effects, including the ability to modulate cytokine production and to exert a positive influence upon regeneration processes. In addition, based on visual inspection, vulvoscopy and morphohistochemical examination results (evaluation criteria: skin thickness, number of collagen fibers, severity of fibrosis and sclerosis, etc.), the corresponding subgroups were classified within the II group, i.e., 2.1 (minimal sclerotic signs, n = 14), and 2.2 (pronounced sclerotic signs, n = 20). The control group consisted of conditionally healthy women, without history or presence of vulvar pathology (n = 30), with an age ranging from 20 to 50 years. Along with cytokine assessment by enzyme immunoassay, the study used the data of clinical examination (anamnesis collection, examination, palpation, vulvoscopy), as well as complex morphohistochemical evaluation of vulvar tissues. In atrophic variant, we have observed an increase in plasma IL-23 content, along with decreased TNFα; in lichen sclerosis, a maximal increase in IL-20, IL-23, and IFNγ was revealed; in sclerotic form of sclerotic lichen variant with severe sclerotic features, maximally enhanced IL-20, IL-23, TNFα, IFNγ, along with minimal levels of IL-10 was registered, as compared with other groups. Immunotherapy using Derinate resulted into significant reduction in the clinical manifestations in sclerotic lichen, i.e., itching of the vulva and dyspareunia, as well as normalization of cytokine indexes. Our studies have demonstrated an opportunity of using plasma concentrations of IL-20, IL-23, IL-10, TNFα, IFNγ as biomarkers of sclerotic lichen variants, and as laboratory criteria for efficiency of immunotherapy.E. V. KolesnikovaA. V. ZharovG. A. PenzhoyanSPb RAACIarticlebenign vulvar diseasessclerotic lichencytokinesimmunotherapypreparations of nucleic acidsclinical and immunological efficiencyoptimization of diagnosisoptimization of treatmentImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 23, Iss 1, Pp 63-72 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	benign vulvar diseases sclerotic lichen cytokines immunotherapy preparations of nucleic acids clinical and immunological efficiency optimization of diagnosis optimization of treatment Immunologic diseases. Allergy RC581-607
spellingShingle	benign vulvar diseases sclerotic lichen cytokines immunotherapy preparations of nucleic acids clinical and immunological efficiency optimization of diagnosis optimization of treatment Immunologic diseases. Allergy RC581-607 E. V. Kolesnikova A. V. Zharov G. A. Penzhoyan Role of cytokines in pathogenesis, diagnosis and efficiency evaluation of immunotherapy in various variants of sclerotic lichen in women
description	Our aim was to evaluate diagnostic and pathogenetic significance of plasma cytokines (IL-20, IL-23, IL-10, TNFα, IFNγ) in the patients with various clinical and histological variants of sclerotic lichen and to assess opportunity for their use as effectiveness criteria of immunotherapy for this disease using a drug based on eukaryotic deoxyribonucleic acid (Derinat). The prospective cohort study included assessment of the clinical manifestations (itching and dyspareunia) and measurement of blood cytokine contents (IL-20, IL-23, IL-10, TNFα, IFNγ) in women (n = 114) with various clinical variants of sclerotic lichen (atrophic, sclerotic and sclerotic-atrophic) before and after immunotherapy with a nucleic acid-based drug (Derinat). Derinat was chosen due to the fact of being an agonist of Toll-like receptors, and a number of immunoregulatory effects, including the ability to modulate cytokine production and to exert a positive influence upon regeneration processes. In addition, based on visual inspection, vulvoscopy and morphohistochemical examination results (evaluation criteria: skin thickness, number of collagen fibers, severity of fibrosis and sclerosis, etc.), the corresponding subgroups were classified within the II group, i.e., 2.1 (minimal sclerotic signs, n = 14), and 2.2 (pronounced sclerotic signs, n = 20). The control group consisted of conditionally healthy women, without history or presence of vulvar pathology (n = 30), with an age ranging from 20 to 50 years. Along with cytokine assessment by enzyme immunoassay, the study used the data of clinical examination (anamnesis collection, examination, palpation, vulvoscopy), as well as complex morphohistochemical evaluation of vulvar tissues. In atrophic variant, we have observed an increase in plasma IL-23 content, along with decreased TNFα; in lichen sclerosis, a maximal increase in IL-20, IL-23, and IFNγ was revealed; in sclerotic form of sclerotic lichen variant with severe sclerotic features, maximally enhanced IL-20, IL-23, TNFα, IFNγ, along with minimal levels of IL-10 was registered, as compared with other groups. Immunotherapy using Derinate resulted into significant reduction in the clinical manifestations in sclerotic lichen, i.e., itching of the vulva and dyspareunia, as well as normalization of cytokine indexes. Our studies have demonstrated an opportunity of using plasma concentrations of IL-20, IL-23, IL-10, TNFα, IFNγ as biomarkers of sclerotic lichen variants, and as laboratory criteria for efficiency of immunotherapy.
format	article
author	E. V. Kolesnikova A. V. Zharov G. A. Penzhoyan
author_facet	E. V. Kolesnikova A. V. Zharov G. A. Penzhoyan
author_sort	E. V. Kolesnikova
title	Role of cytokines in pathogenesis, diagnosis and efficiency evaluation of immunotherapy in various variants of sclerotic lichen in women
title_short	Role of cytokines in pathogenesis, diagnosis and efficiency evaluation of immunotherapy in various variants of sclerotic lichen in women
title_full	Role of cytokines in pathogenesis, diagnosis and efficiency evaluation of immunotherapy in various variants of sclerotic lichen in women
title_fullStr	Role of cytokines in pathogenesis, diagnosis and efficiency evaluation of immunotherapy in various variants of sclerotic lichen in women
title_full_unstemmed	Role of cytokines in pathogenesis, diagnosis and efficiency evaluation of immunotherapy in various variants of sclerotic lichen in women
title_sort	role of cytokines in pathogenesis, diagnosis and efficiency evaluation of immunotherapy in various variants of sclerotic lichen in women
publisher	SPb RAACI
publishDate	2021
url	https://doaj.org/article/1114502e29764a598b4fdecbd65fafa5
work_keys_str_mv	AT evkolesnikova roleofcytokinesinpathogenesisdiagnosisandefficiencyevaluationofimmunotherapyinvariousvariantsofscleroticlicheninwomen AT avzharov roleofcytokinesinpathogenesisdiagnosisandefficiencyevaluationofimmunotherapyinvariousvariantsofscleroticlicheninwomen AT gapenzhoyan roleofcytokinesinpathogenesisdiagnosisandefficiencyevaluationofimmunotherapyinvariousvariantsofscleroticlicheninwomen
_version_	1718422272654442496

Role of cytokines in pathogenesis, diagnosis and efficiency evaluation of immunotherapy in various variants of sclerotic lichen in women

Ejemplares similares