LED-fluorescence microscopy for diagnosis of pulmonary tuberculosis under programmatic conditions in India.

<h4>Background</h4>Light-emitting diode fluorescence microscopy (LED-FM) has been shown to be more sensitive than conventional bright field microscopy using Ziehl-Neelsen (ZN) stain in detecting sputum smear positive tuberculosis in controlled laboratory conditions. In 2012, Auramine O s...

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Autores principales:	Lord Wasim Reza, Srinath Satyanarayna, Donald A Enarson, Ajay M V Kumar, Karuna Sagili, Sujeet Kumar, Levi Anand Prabhakar, N M Devendrappa, Ashish Pandey, Nevin Wilson, Sarabjit Chadha, Badri Thapa, Kuldeep Singh Sachdeva, Mohan P Kohli
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/11174a8488c5423e8e3de29d4b29747a
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spelling	oai:doaj.org-article:11174a8488c5423e8e3de29d4b29747a2021-11-18T08:51:51ZLED-fluorescence microscopy for diagnosis of pulmonary tuberculosis under programmatic conditions in India.1932-620310.1371/journal.pone.0075566https://doaj.org/article/11174a8488c5423e8e3de29d4b29747a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24130719/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Light-emitting diode fluorescence microscopy (LED-FM) has been shown to be more sensitive than conventional bright field microscopy using Ziehl-Neelsen (ZN) stain in detecting sputum smear positive tuberculosis in controlled laboratory conditions. In 2012, Auramine O staining based LED-FM replaced conventional ZN microscopy in 200 designated microscopy centres (DMC) of medical colleges operating in collaboration with India's Revised National Tuberculosis Control Programme. We aimed to assess the impact of introduction of LED-FM services on sputum smear positive case detection under program conditions.<h4>Methods</h4>This was a before and after comparison study. In 15 randomly selected medical college DMCs, all presumptive TB patients who underwent sputum smear examination in the years 2011 (before LED-FM) and 2012 (after LED-FM) were compared. An additional 15 comparable DMCs that implemented conventional ZN sputum smear microscopy were also selected for comparison between 2011 and 2012.<h4>Results</h4>The proportion of presumptive TB patients (PTP)found sputum smear positive increased by 30%- from 13.6% (3432/25159) in 2011 to 17.8% (4706/26426) in 2012 (P value <0.01) in the sites that implemented LED-FM microscopy, whereas in DMCs where the ZN staining procedure is followed the proportion of sputum smear positive had remained unchanged (13.0%versus 12.6%;P value0.31).<h4>Conclusion</h4>Use of LED-FM significantly increased the proportion of smear positive cases among presumptive TB patients under routine program conditions in high workload laboratories. The study provides operational evidence needed to scale-up the use of LED-FM in similar settings in India and beyond.Lord Wasim RezaSrinath SatyanaraynaDonald A EnarsonAjay M V KumarKaruna SagiliSujeet KumarLevi Anand PrabhakarN M DevendrappaAshish PandeyNevin WilsonSarabjit ChadhaBadri ThapaKuldeep Singh SachdevaMohan P KohliPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e75566 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lord Wasim Reza Srinath Satyanarayna Donald A Enarson Ajay M V Kumar Karuna Sagili Sujeet Kumar Levi Anand Prabhakar N M Devendrappa Ashish Pandey Nevin Wilson Sarabjit Chadha Badri Thapa Kuldeep Singh Sachdeva Mohan P Kohli LED-fluorescence microscopy for diagnosis of pulmonary tuberculosis under programmatic conditions in India.
description	<h4>Background</h4>Light-emitting diode fluorescence microscopy (LED-FM) has been shown to be more sensitive than conventional bright field microscopy using Ziehl-Neelsen (ZN) stain in detecting sputum smear positive tuberculosis in controlled laboratory conditions. In 2012, Auramine O staining based LED-FM replaced conventional ZN microscopy in 200 designated microscopy centres (DMC) of medical colleges operating in collaboration with India's Revised National Tuberculosis Control Programme. We aimed to assess the impact of introduction of LED-FM services on sputum smear positive case detection under program conditions.<h4>Methods</h4>This was a before and after comparison study. In 15 randomly selected medical college DMCs, all presumptive TB patients who underwent sputum smear examination in the years 2011 (before LED-FM) and 2012 (after LED-FM) were compared. An additional 15 comparable DMCs that implemented conventional ZN sputum smear microscopy were also selected for comparison between 2011 and 2012.<h4>Results</h4>The proportion of presumptive TB patients (PTP)found sputum smear positive increased by 30%- from 13.6% (3432/25159) in 2011 to 17.8% (4706/26426) in 2012 (P value <0.01) in the sites that implemented LED-FM microscopy, whereas in DMCs where the ZN staining procedure is followed the proportion of sputum smear positive had remained unchanged (13.0%versus 12.6%;P value0.31).<h4>Conclusion</h4>Use of LED-FM significantly increased the proportion of smear positive cases among presumptive TB patients under routine program conditions in high workload laboratories. The study provides operational evidence needed to scale-up the use of LED-FM in similar settings in India and beyond.
format	article
author	Lord Wasim Reza Srinath Satyanarayna Donald A Enarson Ajay M V Kumar Karuna Sagili Sujeet Kumar Levi Anand Prabhakar N M Devendrappa Ashish Pandey Nevin Wilson Sarabjit Chadha Badri Thapa Kuldeep Singh Sachdeva Mohan P Kohli
author_facet	Lord Wasim Reza Srinath Satyanarayna Donald A Enarson Ajay M V Kumar Karuna Sagili Sujeet Kumar Levi Anand Prabhakar N M Devendrappa Ashish Pandey Nevin Wilson Sarabjit Chadha Badri Thapa Kuldeep Singh Sachdeva Mohan P Kohli
author_sort	Lord Wasim Reza
title	LED-fluorescence microscopy for diagnosis of pulmonary tuberculosis under programmatic conditions in India.
title_short	LED-fluorescence microscopy for diagnosis of pulmonary tuberculosis under programmatic conditions in India.
title_full	LED-fluorescence microscopy for diagnosis of pulmonary tuberculosis under programmatic conditions in India.
title_fullStr	LED-fluorescence microscopy for diagnosis of pulmonary tuberculosis under programmatic conditions in India.
title_full_unstemmed	LED-fluorescence microscopy for diagnosis of pulmonary tuberculosis under programmatic conditions in India.
title_sort	led-fluorescence microscopy for diagnosis of pulmonary tuberculosis under programmatic conditions in india.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/11174a8488c5423e8e3de29d4b29747a
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LED-fluorescence microscopy for diagnosis of pulmonary tuberculosis under programmatic conditions in India.

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