The effect of intravitreal bevacizumab and ranibizumab on cutaneous tensile strength during wound healing

John B Christoforidis,1 Jillian Wang,2 Angela Jiang,2 James Willard,5 Cedric Pratt,2 Mahmoud Abdel-Rasoul,3 Sashwati Roy,4 Heather Powell51Department of Ophthalmology and Vision Science, College of Medicine, The University of Arizona, Tucson, AZ, USA; 2Department of Ophthalmology, College of Medicin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Christoforidis JB, Wang J, Jiang A, Willard J, Pratt C, Abdel-Rasoul M, Roy S, Powell HM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/111c96a64af84ac381df1d195127fd4e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:111c96a64af84ac381df1d195127fd4e
record_format	dspace
spelling	oai:doaj.org-article:111c96a64af84ac381df1d195127fd4e2021-12-02T07:52:14ZThe effect of intravitreal bevacizumab and ranibizumab on cutaneous tensile strength during wound healing1177-54671177-5483https://doaj.org/article/111c96a64af84ac381df1d195127fd4e2013-01-01T00:00:00Zhttp://www.dovepress.com/the-effect-of-intravitreal-bevacizumab-and-ranibizumab-on-cutaneous-te-a12059https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483John B Christoforidis,1 Jillian Wang,2 Angela Jiang,2 James Willard,5 Cedric Pratt,2 Mahmoud Abdel-Rasoul,3 Sashwati Roy,4 Heather Powell51Department of Ophthalmology and Vision Science, College of Medicine, The University of Arizona, Tucson, AZ, USA; 2Department of Ophthalmology, College of Medicine, The Ohio State University, Columbus, OH, USA; 3Center for Biostatistics, The Ohio State University, Columbus, OH, USA; 4Center Surgery, The Ohio State University, Columbus, OH, USA; 5Department of Materials Science, College of Engineering, The Ohio State University, Columbus, OH, USAPurpose: To investigate the effect of intravitreal bevacizumab and ranibizumab on wound tension and by histopathology during cutaneous wound healing in a rabbit model and to compare this effect to placebo intravitreal saline controls 1 and 2 weeks following intravitreal injection.Methods: A total of 120 New Zealand white rabbits were randomly assigned to one of three treatment groups each consisting of 40 rabbits. Each group received intravitreal injections of bevacizumab, ranibizumab, or normal saline. Immediately afterwards, each rabbit underwent four 6 mm full-thickness dermatologic punch biopsies. Twenty rabbits from each agent group underwent wound harvesting on day 7 or day 14. The skin samples were stained for CD34 for vascular endothelial cells on day 7, and maximal wound tensile load was measured on days 7 and 14. Quantitative assessment of mean neovascularization (MNV) scores was obtained from 10 contiguous biopsy margin 400&times; fields of CD34-stained sections by two independent observers.Results: Wound tension reading means (N) with standard error and adjusted P-values on day 7 were: saline placebos, 7.46 &plusmn; 0.87; bevacizumab, 4.50 &plusmn; 0.88 (P = 0.041); and ranibizumab, 4.67 &plusmn; 0.84 (P = 0.025). On day 14 these were: saline placebos, 7.34 &plusmn; 0.55; bevacizumab, 6.05 &plusmn; 0.54 (P = 0.18); and ranibizumab 7.99 &plusmn; 0.54 (P = 0.40). MNV scores in CD34 stained sections were: saline controls, 18.31 &plusmn; 0.43; bevacizumab, 11.02 &plusmn; 0.45 (P &lt; 0.0001); and ranibizumab, 13.55 &plusmn; 0.43 (P &lt; 0.0001). The interobserver correlation coefficient was 0.928.Conclusion: At day 7, both anti&ndash;vascular endothelial growth factor (anti-VEGF) agents had significantly suppressed MNV scores and exerted a significant reduction of cutaneous wound tensile strength compared with saline controls. At day 14, neither agent produced a significant effect on tensile wound strength. Since angiogenesis is an integral component of the proliferative phase of wound healing, we encourage clinicians to be aware of their patients&#39; recent surgical history during intravitreal anti-VEGF therapy and to consider refraining from their use during the perioperative period.Keywords: wound healing, tensile strength, bevacizumab, ranibizumabChristoforidis JBWang JJiang AWillard JPratt CAbdel-Rasoul MRoy SPowell HMDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2013, Iss default, Pp 185-191 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Christoforidis JB Wang J Jiang A Willard J Pratt C Abdel-Rasoul M Roy S Powell HM The effect of intravitreal bevacizumab and ranibizumab on cutaneous tensile strength during wound healing
description	John B Christoforidis,1 Jillian Wang,2 Angela Jiang,2 James Willard,5 Cedric Pratt,2 Mahmoud Abdel-Rasoul,3 Sashwati Roy,4 Heather Powell51Department of Ophthalmology and Vision Science, College of Medicine, The University of Arizona, Tucson, AZ, USA; 2Department of Ophthalmology, College of Medicine, The Ohio State University, Columbus, OH, USA; 3Center for Biostatistics, The Ohio State University, Columbus, OH, USA; 4Center Surgery, The Ohio State University, Columbus, OH, USA; 5Department of Materials Science, College of Engineering, The Ohio State University, Columbus, OH, USAPurpose: To investigate the effect of intravitreal bevacizumab and ranibizumab on wound tension and by histopathology during cutaneous wound healing in a rabbit model and to compare this effect to placebo intravitreal saline controls 1 and 2 weeks following intravitreal injection.Methods: A total of 120 New Zealand white rabbits were randomly assigned to one of three treatment groups each consisting of 40 rabbits. Each group received intravitreal injections of bevacizumab, ranibizumab, or normal saline. Immediately afterwards, each rabbit underwent four 6 mm full-thickness dermatologic punch biopsies. Twenty rabbits from each agent group underwent wound harvesting on day 7 or day 14. The skin samples were stained for CD34 for vascular endothelial cells on day 7, and maximal wound tensile load was measured on days 7 and 14. Quantitative assessment of mean neovascularization (MNV) scores was obtained from 10 contiguous biopsy margin 400&times; fields of CD34-stained sections by two independent observers.Results: Wound tension reading means (N) with standard error and adjusted P-values on day 7 were: saline placebos, 7.46 &plusmn; 0.87; bevacizumab, 4.50 &plusmn; 0.88 (P = 0.041); and ranibizumab, 4.67 &plusmn; 0.84 (P = 0.025). On day 14 these were: saline placebos, 7.34 &plusmn; 0.55; bevacizumab, 6.05 &plusmn; 0.54 (P = 0.18); and ranibizumab 7.99 &plusmn; 0.54 (P = 0.40). MNV scores in CD34 stained sections were: saline controls, 18.31 &plusmn; 0.43; bevacizumab, 11.02 &plusmn; 0.45 (P &lt; 0.0001); and ranibizumab, 13.55 &plusmn; 0.43 (P &lt; 0.0001). The interobserver correlation coefficient was 0.928.Conclusion: At day 7, both anti&ndash;vascular endothelial growth factor (anti-VEGF) agents had significantly suppressed MNV scores and exerted a significant reduction of cutaneous wound tensile strength compared with saline controls. At day 14, neither agent produced a significant effect on tensile wound strength. Since angiogenesis is an integral component of the proliferative phase of wound healing, we encourage clinicians to be aware of their patients&#39; recent surgical history during intravitreal anti-VEGF therapy and to consider refraining from their use during the perioperative period.Keywords: wound healing, tensile strength, bevacizumab, ranibizumab
format	article
author	Christoforidis JB Wang J Jiang A Willard J Pratt C Abdel-Rasoul M Roy S Powell HM
author_facet	Christoforidis JB Wang J Jiang A Willard J Pratt C Abdel-Rasoul M Roy S Powell HM
author_sort	Christoforidis JB
title	The effect of intravitreal bevacizumab and ranibizumab on cutaneous tensile strength during wound healing
title_short	The effect of intravitreal bevacizumab and ranibizumab on cutaneous tensile strength during wound healing
title_full	The effect of intravitreal bevacizumab and ranibizumab on cutaneous tensile strength during wound healing
title_fullStr	The effect of intravitreal bevacizumab and ranibizumab on cutaneous tensile strength during wound healing
title_full_unstemmed	The effect of intravitreal bevacizumab and ranibizumab on cutaneous tensile strength during wound healing
title_sort	effect of intravitreal bevacizumab and ranibizumab on cutaneous tensile strength during wound healing
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/111c96a64af84ac381df1d195127fd4e
work_keys_str_mv	AT christoforidisjb theeffectofintravitrealbevacizumabandranibizumaboncutaneoustensilestrengthduringwoundhealing AT wangj theeffectofintravitrealbevacizumabandranibizumaboncutaneoustensilestrengthduringwoundhealing AT jianga theeffectofintravitrealbevacizumabandranibizumaboncutaneoustensilestrengthduringwoundhealing AT willardj theeffectofintravitrealbevacizumabandranibizumaboncutaneoustensilestrengthduringwoundhealing AT prattc theeffectofintravitrealbevacizumabandranibizumaboncutaneoustensilestrengthduringwoundhealing AT abdelrasoulm theeffectofintravitrealbevacizumabandranibizumaboncutaneoustensilestrengthduringwoundhealing AT roys theeffectofintravitrealbevacizumabandranibizumaboncutaneoustensilestrengthduringwoundhealing AT powellhm theeffectofintravitrealbevacizumabandranibizumaboncutaneoustensilestrengthduringwoundhealing AT christoforidisjb effectofintravitrealbevacizumabandranibizumaboncutaneoustensilestrengthduringwoundhealing AT wangj effectofintravitrealbevacizumabandranibizumaboncutaneoustensilestrengthduringwoundhealing AT jianga effectofintravitrealbevacizumabandranibizumaboncutaneoustensilestrengthduringwoundhealing AT willardj effectofintravitrealbevacizumabandranibizumaboncutaneoustensilestrengthduringwoundhealing AT prattc effectofintravitrealbevacizumabandranibizumaboncutaneoustensilestrengthduringwoundhealing AT abdelrasoulm effectofintravitrealbevacizumabandranibizumaboncutaneoustensilestrengthduringwoundhealing AT roys effectofintravitrealbevacizumabandranibizumaboncutaneoustensilestrengthduringwoundhealing AT powellhm effectofintravitrealbevacizumabandranibizumaboncutaneoustensilestrengthduringwoundhealing
_version_	1718399084616744960

The effect of intravitreal bevacizumab and ranibizumab on cutaneous tensile strength during wound healing

Ejemplares similares