Digital gene expression profiling by 5'-end sequencing of cDNAs during reprogramming in the moss Physcomitrella patens.

Stem cells self-renew and repeatedly produce differentiated cells during development and growth. The differentiated cells can be converted into stem cells in some metazoans and land plants with appropriate treatments. After leaves of the moss Physcomitrella patens are excised, leaf cells reenter the...

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Autores principales:	Tomoaki Nishiyama, Kaori Miyawaki, Masumi Ohshima, Kari Thompson, Akitomo Nagashima, Mitsuyasu Hasebe, Tetsuya Kurata
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1121974420a3403a9941bc7d9a24cedb
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spelling	oai:doaj.org-article:1121974420a3403a9941bc7d9a24cedb2021-11-18T07:19:38ZDigital gene expression profiling by 5'-end sequencing of cDNAs during reprogramming in the moss Physcomitrella patens.1932-620310.1371/journal.pone.0036471https://doaj.org/article/1121974420a3403a9941bc7d9a24cedb2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22574165/?tool=EBIhttps://doaj.org/toc/1932-6203Stem cells self-renew and repeatedly produce differentiated cells during development and growth. The differentiated cells can be converted into stem cells in some metazoans and land plants with appropriate treatments. After leaves of the moss Physcomitrella patens are excised, leaf cells reenter the cell cycle and commence tip growth, which is characteristic of stem cells called chloronema apical cells. To understand the underlying molecular mechanisms, a digital gene expression profiling method using mRNA 5'-end tags (5'-DGE) was established. The 5'-DGE method produced reproducible data with a dynamic range of four orders that correlated well with qRT-PCR measurements. After the excision of leaves, the expression levels of 11% of the transcripts changed significantly within 6 h. Genes involved in stress responses and proteolysis were induced and those involved in metabolism, including photosynthesis, were reduced. The later processes of reprogramming involved photosynthesis recovery and higher macromolecule biosynthesis, including of RNA and proteins. Auxin and cytokinin signaling pathways, which are activated during stem cell formation via callus in flowering plants, are also activated during reprogramming in P. patens, although no exogenous phytohormone is applied in the moss system, suggesting that an intrinsic phytohormone regulatory system may be used in the moss.Tomoaki NishiyamaKaori MiyawakiMasumi OhshimaKari ThompsonAkitomo NagashimaMitsuyasu HasebeTetsuya KurataPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e36471 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tomoaki Nishiyama Kaori Miyawaki Masumi Ohshima Kari Thompson Akitomo Nagashima Mitsuyasu Hasebe Tetsuya Kurata Digital gene expression profiling by 5'-end sequencing of cDNAs during reprogramming in the moss Physcomitrella patens.
description	Stem cells self-renew and repeatedly produce differentiated cells during development and growth. The differentiated cells can be converted into stem cells in some metazoans and land plants with appropriate treatments. After leaves of the moss Physcomitrella patens are excised, leaf cells reenter the cell cycle and commence tip growth, which is characteristic of stem cells called chloronema apical cells. To understand the underlying molecular mechanisms, a digital gene expression profiling method using mRNA 5'-end tags (5'-DGE) was established. The 5'-DGE method produced reproducible data with a dynamic range of four orders that correlated well with qRT-PCR measurements. After the excision of leaves, the expression levels of 11% of the transcripts changed significantly within 6 h. Genes involved in stress responses and proteolysis were induced and those involved in metabolism, including photosynthesis, were reduced. The later processes of reprogramming involved photosynthesis recovery and higher macromolecule biosynthesis, including of RNA and proteins. Auxin and cytokinin signaling pathways, which are activated during stem cell formation via callus in flowering plants, are also activated during reprogramming in P. patens, although no exogenous phytohormone is applied in the moss system, suggesting that an intrinsic phytohormone regulatory system may be used in the moss.
format	article
author	Tomoaki Nishiyama Kaori Miyawaki Masumi Ohshima Kari Thompson Akitomo Nagashima Mitsuyasu Hasebe Tetsuya Kurata
author_facet	Tomoaki Nishiyama Kaori Miyawaki Masumi Ohshima Kari Thompson Akitomo Nagashima Mitsuyasu Hasebe Tetsuya Kurata
author_sort	Tomoaki Nishiyama
title	Digital gene expression profiling by 5'-end sequencing of cDNAs during reprogramming in the moss Physcomitrella patens.
title_short	Digital gene expression profiling by 5'-end sequencing of cDNAs during reprogramming in the moss Physcomitrella patens.
title_full	Digital gene expression profiling by 5'-end sequencing of cDNAs during reprogramming in the moss Physcomitrella patens.
title_fullStr	Digital gene expression profiling by 5'-end sequencing of cDNAs during reprogramming in the moss Physcomitrella patens.
title_full_unstemmed	Digital gene expression profiling by 5'-end sequencing of cDNAs during reprogramming in the moss Physcomitrella patens.
title_sort	digital gene expression profiling by 5'-end sequencing of cdnas during reprogramming in the moss physcomitrella patens.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/1121974420a3403a9941bc7d9a24cedb
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Digital gene expression profiling by 5'-end sequencing of cDNAs during reprogramming in the moss Physcomitrella patens.

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