Cell visco-elasticity measured with AFM and optical trapping at sub-micrometer deformations.

The measurement of the elastic properties of cells is widely used as an indicator for cellular changes during differentiation, upon drug treatment, or resulting from the interaction with the supporting matrix. Elasticity is routinely quantified by indenting the cell with a probe of an AFM while appl...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Schanila Nawaz, Paula Sánchez, Kai Bodensiek, Sai Li, Mikael Simons, Iwan A T Schaap
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/11243d4d5e6445fe98f05adc71b2fff2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:11243d4d5e6445fe98f05adc71b2fff2
record_format	dspace
spelling	oai:doaj.org-article:11243d4d5e6445fe98f05adc71b2fff22021-11-18T07:04:56ZCell visco-elasticity measured with AFM and optical trapping at sub-micrometer deformations.1932-620310.1371/journal.pone.0045297https://doaj.org/article/11243d4d5e6445fe98f05adc71b2fff22012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23028915/?tool=EBIhttps://doaj.org/toc/1932-6203The measurement of the elastic properties of cells is widely used as an indicator for cellular changes during differentiation, upon drug treatment, or resulting from the interaction with the supporting matrix. Elasticity is routinely quantified by indenting the cell with a probe of an AFM while applying nano-Newton forces. Because the resulting deformations are in the micrometer range, the measurements will be affected by the finite thickness of the cell, viscous effects and even cell damage induced by the experiment itself. Here, we have analyzed the response of single 3T3 fibroblasts that were indented with a micrometer-sized bead attached to an AFM cantilever at forces from 30-600 pN, resulting in indentations ranging from 0.2 to 1.2 micrometer. To investigate the cellular response at lower forces up to 10 pN, we developed an optical trap to indent the cell in vertical direction, normal to the plane of the coverslip. Deformations of up to two hundred nanometers achieved at forces of up to 30 pN showed a reversible, thus truly elastic response that was independent on the rate of deformation. We found that at such small deformations, the elastic modulus of 100 Pa is largely determined by the presence of the actin cortex. At higher indentations, viscous effects led to an increase of the apparent elastic modulus. This viscous contribution that followed a weak power law, increased at larger cell indentations. Both AFM and optical trapping indentation experiments give consistent results for the cell elasticity. Optical trapping has the benefit of a lower force noise, which allows a more accurate determination of the absolute indentation. The combination of both techniques allows the investigation of single cells at small and large indentations and enables the separation of their viscous and elastic components.Schanila NawazPaula SánchezKai BodensiekSai LiMikael SimonsIwan A T SchaapPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 9, p e45297 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Schanila Nawaz Paula Sánchez Kai Bodensiek Sai Li Mikael Simons Iwan A T Schaap Cell visco-elasticity measured with AFM and optical trapping at sub-micrometer deformations.
description	The measurement of the elastic properties of cells is widely used as an indicator for cellular changes during differentiation, upon drug treatment, or resulting from the interaction with the supporting matrix. Elasticity is routinely quantified by indenting the cell with a probe of an AFM while applying nano-Newton forces. Because the resulting deformations are in the micrometer range, the measurements will be affected by the finite thickness of the cell, viscous effects and even cell damage induced by the experiment itself. Here, we have analyzed the response of single 3T3 fibroblasts that were indented with a micrometer-sized bead attached to an AFM cantilever at forces from 30-600 pN, resulting in indentations ranging from 0.2 to 1.2 micrometer. To investigate the cellular response at lower forces up to 10 pN, we developed an optical trap to indent the cell in vertical direction, normal to the plane of the coverslip. Deformations of up to two hundred nanometers achieved at forces of up to 30 pN showed a reversible, thus truly elastic response that was independent on the rate of deformation. We found that at such small deformations, the elastic modulus of 100 Pa is largely determined by the presence of the actin cortex. At higher indentations, viscous effects led to an increase of the apparent elastic modulus. This viscous contribution that followed a weak power law, increased at larger cell indentations. Both AFM and optical trapping indentation experiments give consistent results for the cell elasticity. Optical trapping has the benefit of a lower force noise, which allows a more accurate determination of the absolute indentation. The combination of both techniques allows the investigation of single cells at small and large indentations and enables the separation of their viscous and elastic components.
format	article
author	Schanila Nawaz Paula Sánchez Kai Bodensiek Sai Li Mikael Simons Iwan A T Schaap
author_facet	Schanila Nawaz Paula Sánchez Kai Bodensiek Sai Li Mikael Simons Iwan A T Schaap
author_sort	Schanila Nawaz
title	Cell visco-elasticity measured with AFM and optical trapping at sub-micrometer deformations.
title_short	Cell visco-elasticity measured with AFM and optical trapping at sub-micrometer deformations.
title_full	Cell visco-elasticity measured with AFM and optical trapping at sub-micrometer deformations.
title_fullStr	Cell visco-elasticity measured with AFM and optical trapping at sub-micrometer deformations.
title_full_unstemmed	Cell visco-elasticity measured with AFM and optical trapping at sub-micrometer deformations.
title_sort	cell visco-elasticity measured with afm and optical trapping at sub-micrometer deformations.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/11243d4d5e6445fe98f05adc71b2fff2
work_keys_str_mv	AT schanilanawaz cellviscoelasticitymeasuredwithafmandopticaltrappingatsubmicrometerdeformations AT paulasanchez cellviscoelasticitymeasuredwithafmandopticaltrappingatsubmicrometerdeformations AT kaibodensiek cellviscoelasticitymeasuredwithafmandopticaltrappingatsubmicrometerdeformations AT saili cellviscoelasticitymeasuredwithafmandopticaltrappingatsubmicrometerdeformations AT mikaelsimons cellviscoelasticitymeasuredwithafmandopticaltrappingatsubmicrometerdeformations AT iwanatschaap cellviscoelasticitymeasuredwithafmandopticaltrappingatsubmicrometerdeformations
_version_	1718423961237192704

Cell visco-elasticity measured with AFM and optical trapping at sub-micrometer deformations.

Ejemplares similares