The state, transport, and fate of aboveground terrestrial arthropod eDNA

Abstract Environmental DNA (eDNA) analyses have become invaluable for detecting and monitoring aquatic and terrestrial species and assessing site biodiversity within aquatic environments or soil. Recent studies have extended these techniques by using eDNA to identify the presence of aboveground terr...

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Autores principales:	Rafael E. Valentin, Kathleen E. Kyle, Michael C. Allen, Dustin J. Welbourne, Julie L. Lockwood
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	aboveground degradation environmental DNA filters rainfall UV Environmental sciences GE1-350 Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/1128287af96f4f6881792f8105a36a48
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spelling	oai:doaj.org-article:1128287af96f4f6881792f8105a36a482021-11-23T16:15:24ZThe state, transport, and fate of aboveground terrestrial arthropod eDNA2637-494310.1002/edn3.229https://doaj.org/article/1128287af96f4f6881792f8105a36a482021-11-01T00:00:00Zhttps://doi.org/10.1002/edn3.229https://doaj.org/toc/2637-4943Abstract Environmental DNA (eDNA) analyses have become invaluable for detecting and monitoring aquatic and terrestrial species and assessing site biodiversity within aquatic environments or soil. Recent studies have extended these techniques by using eDNA to identify the presence of aboveground terrestrial arthropods directly from vegetative surfaces. However, while the dynamics of eDNA state, transport, and fate (its “ecology”) have been explored within aquatic environments and soil, they have yet to be explored within aboveground terrestrial systems. Here, we explore the ecology of terrestrial eDNA deposited by fluid‐feeding arthropods on leaf surfaces. We carried out a series of experiments to evaluate the optimal filter pore size for intracellular eDNA collection, how eDNA is affected by rain events, and its degradation rate under different solar radiation conditions. We found that the captured concentration of intracellular eDNA was not significantly affected by an increase in filter pore size, suggesting a wide range of viable pore size options exist for targeting intracellular eDNA. We also found extracellular eDNA from fluid excrement degrades more rapidly than intracellular when exposed to solar radiation, indicating the latter is a more viable target for collection. Finally, we identified that rainfall or mist will remove most terrestrial eDNA present on vegetation surfaces. We provide researchers and environmental managers key insights into successfully designing and carrying out aboveground terrestrial arthropod eDNA surveys that maximize detection probability.Rafael E. ValentinKathleen E. KyleMichael C. AllenDustin J. WelbourneJulie L. LockwoodWileyarticleabovegrounddegradationenvironmental DNAfiltersrainfallUVEnvironmental sciencesGE1-350Microbial ecologyQR100-130ENEnvironmental DNA, Vol 3, Iss 6, Pp 1081-1092 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aboveground degradation environmental DNA filters rainfall UV Environmental sciences GE1-350 Microbial ecology QR100-130
spellingShingle	aboveground degradation environmental DNA filters rainfall UV Environmental sciences GE1-350 Microbial ecology QR100-130 Rafael E. Valentin Kathleen E. Kyle Michael C. Allen Dustin J. Welbourne Julie L. Lockwood The state, transport, and fate of aboveground terrestrial arthropod eDNA
description	Abstract Environmental DNA (eDNA) analyses have become invaluable for detecting and monitoring aquatic and terrestrial species and assessing site biodiversity within aquatic environments or soil. Recent studies have extended these techniques by using eDNA to identify the presence of aboveground terrestrial arthropods directly from vegetative surfaces. However, while the dynamics of eDNA state, transport, and fate (its “ecology”) have been explored within aquatic environments and soil, they have yet to be explored within aboveground terrestrial systems. Here, we explore the ecology of terrestrial eDNA deposited by fluid‐feeding arthropods on leaf surfaces. We carried out a series of experiments to evaluate the optimal filter pore size for intracellular eDNA collection, how eDNA is affected by rain events, and its degradation rate under different solar radiation conditions. We found that the captured concentration of intracellular eDNA was not significantly affected by an increase in filter pore size, suggesting a wide range of viable pore size options exist for targeting intracellular eDNA. We also found extracellular eDNA from fluid excrement degrades more rapidly than intracellular when exposed to solar radiation, indicating the latter is a more viable target for collection. Finally, we identified that rainfall or mist will remove most terrestrial eDNA present on vegetation surfaces. We provide researchers and environmental managers key insights into successfully designing and carrying out aboveground terrestrial arthropod eDNA surveys that maximize detection probability.
format	article
author	Rafael E. Valentin Kathleen E. Kyle Michael C. Allen Dustin J. Welbourne Julie L. Lockwood
author_facet	Rafael E. Valentin Kathleen E. Kyle Michael C. Allen Dustin J. Welbourne Julie L. Lockwood
author_sort	Rafael E. Valentin
title	The state, transport, and fate of aboveground terrestrial arthropod eDNA
title_short	The state, transport, and fate of aboveground terrestrial arthropod eDNA
title_full	The state, transport, and fate of aboveground terrestrial arthropod eDNA
title_fullStr	The state, transport, and fate of aboveground terrestrial arthropod eDNA
title_full_unstemmed	The state, transport, and fate of aboveground terrestrial arthropod eDNA
title_sort	state, transport, and fate of aboveground terrestrial arthropod edna
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/1128287af96f4f6881792f8105a36a48
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The state, transport, and fate of aboveground terrestrial arthropod eDNA

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