Susceptibility and cytokine responses of human neuronal cells to multiple circulating EV-A71 genotypes in India

Abstract Enterovirus-A71 (EV-A71) associated Hand, foot and mouth disease (HFMD) is a highly contagious viral infection affecting children in Asia–Pacific region and has become a major threat to public health. Although several EV-A71 genotypes (C, D, and G) were isolated in India in recent years, no...

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Autores principales:	Madhu Chhanda Mohanty, Swapnil Yashavant Varose, Vinay Kumar Saxena
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/112b628f2e2e4ffa9bcacc329f7aacf1
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spelling	oai:doaj.org-article:112b628f2e2e4ffa9bcacc329f7aacf12021-12-02T19:12:35ZSusceptibility and cytokine responses of human neuronal cells to multiple circulating EV-A71 genotypes in India10.1038/s41598-021-97166-x2045-2322https://doaj.org/article/112b628f2e2e4ffa9bcacc329f7aacf12021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97166-xhttps://doaj.org/toc/2045-2322Abstract Enterovirus-A71 (EV-A71) associated Hand, foot and mouth disease (HFMD) is a highly contagious viral infection affecting children in Asia–Pacific region and has become a major threat to public health. Although several EV-A71 genotypes (C, D, and G) were isolated in India in recent years, no recognizable outbreak of EV-A71 caused HFMD, Acute Flaccid paralysis (AFP) or encephalitis have been reported so far. It is essential to study the pathogenicity or cell tropism of these Indian isolates in order to understand their tendency to cause disease. We investigated the susceptibility and cytokine responses of indigenous EV-A71 genotypes (D and G) isolated from cases of AFP and genotype C viruses isolated from cases of HFMD and encephalitis, in human cells in-vitro. Although all three EV-A71 genotypes could infect and replicate in human muscle and neuronal cells, the genotype D virus showed a delayed response in human neuronal cells. Quantification of cytokine secretion in response to these isolates followed by confirmation with gene expression assays in human neuronal cells revealed significantly higher secretion of pro-inflammatory cytokines TNF-α IL-8, IL-6, IP-10 (p < 0.001) in G genotype infected cells as compared to pathogenic C genotypes whereas the genotype D virus could not induce any of the inflammatory cytokines. These findings will help to better understand the host response to indigenous EV-A71 genotypes for management of future EV-A71 outbreaks in India, if any.Madhu Chhanda MohantySwapnil Yashavant VaroseVinay Kumar SaxenaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Madhu Chhanda Mohanty Swapnil Yashavant Varose Vinay Kumar Saxena Susceptibility and cytokine responses of human neuronal cells to multiple circulating EV-A71 genotypes in India
description	Abstract Enterovirus-A71 (EV-A71) associated Hand, foot and mouth disease (HFMD) is a highly contagious viral infection affecting children in Asia–Pacific region and has become a major threat to public health. Although several EV-A71 genotypes (C, D, and G) were isolated in India in recent years, no recognizable outbreak of EV-A71 caused HFMD, Acute Flaccid paralysis (AFP) or encephalitis have been reported so far. It is essential to study the pathogenicity or cell tropism of these Indian isolates in order to understand their tendency to cause disease. We investigated the susceptibility and cytokine responses of indigenous EV-A71 genotypes (D and G) isolated from cases of AFP and genotype C viruses isolated from cases of HFMD and encephalitis, in human cells in-vitro. Although all three EV-A71 genotypes could infect and replicate in human muscle and neuronal cells, the genotype D virus showed a delayed response in human neuronal cells. Quantification of cytokine secretion in response to these isolates followed by confirmation with gene expression assays in human neuronal cells revealed significantly higher secretion of pro-inflammatory cytokines TNF-α IL-8, IL-6, IP-10 (p < 0.001) in G genotype infected cells as compared to pathogenic C genotypes whereas the genotype D virus could not induce any of the inflammatory cytokines. These findings will help to better understand the host response to indigenous EV-A71 genotypes for management of future EV-A71 outbreaks in India, if any.
format	article
author	Madhu Chhanda Mohanty Swapnil Yashavant Varose Vinay Kumar Saxena
author_facet	Madhu Chhanda Mohanty Swapnil Yashavant Varose Vinay Kumar Saxena
author_sort	Madhu Chhanda Mohanty
title	Susceptibility and cytokine responses of human neuronal cells to multiple circulating EV-A71 genotypes in India
title_short	Susceptibility and cytokine responses of human neuronal cells to multiple circulating EV-A71 genotypes in India
title_full	Susceptibility and cytokine responses of human neuronal cells to multiple circulating EV-A71 genotypes in India
title_fullStr	Susceptibility and cytokine responses of human neuronal cells to multiple circulating EV-A71 genotypes in India
title_full_unstemmed	Susceptibility and cytokine responses of human neuronal cells to multiple circulating EV-A71 genotypes in India
title_sort	susceptibility and cytokine responses of human neuronal cells to multiple circulating ev-a71 genotypes in india
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/112b628f2e2e4ffa9bcacc329f7aacf1
work_keys_str_mv	AT madhuchhandamohanty susceptibilityandcytokineresponsesofhumanneuronalcellstomultiplecirculatingeva71genotypesinindia AT swapnilyashavantvarose susceptibilityandcytokineresponsesofhumanneuronalcellstomultiplecirculatingeva71genotypesinindia AT vinaykumarsaxena susceptibilityandcytokineresponsesofhumanneuronalcellstomultiplecirculatingeva71genotypesinindia
_version_	1718377010973114368

Susceptibility and cytokine responses of human neuronal cells to multiple circulating EV-A71 genotypes in India

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