Study of the solubilisation process of bacterial model membranes induced by DDAO

Solubilisation of two bacterial model membranes induced by N,N-dimethyl-1-dodecanamine-N-oxide (DDAO) was studied. The first model membrane consisted of a mixture of palmitoyloleoylphosphatidylethanolamine (POPE) and palmitoyloleoylphosphatidylglycerol (POPG) in a molar ratio 0.6:0.4 mol/mol, and a...

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Autores principales:	Želinská K., Gallová J.
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2021
Materias:	n,n-dimethyl-1-dodecanamine-n-oxide solubilisation bacterial model membranes cardiolipin Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/11330af00ecb4e9893411ecf3ca9d8e6
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spelling	oai:doaj.org-article:11330af00ecb4e9893411ecf3ca9d8e62021-12-05T14:11:05ZStudy of the solubilisation process of bacterial model membranes induced by DDAO2453-672510.2478/afpuc-2020-0019https://doaj.org/article/11330af00ecb4e9893411ecf3ca9d8e62021-05-01T00:00:00Zhttps://doi.org/10.2478/afpuc-2020-0019https://doaj.org/toc/2453-6725Solubilisation of two bacterial model membranes induced by N,N-dimethyl-1-dodecanamine-N-oxide (DDAO) was studied. The first model membrane consisted of a mixture of palmitoyloleoylphosphatidylethanolamine (POPE) and palmitoyloleoylphosphatidylglycerol (POPG) in a molar ratio 0.6:0.4 mol/mol, and a second model membrane was enriched with tetraoleoylcardiolipin (TOCL) with a composition POPE-POPG-TOCL = 0.67:0.23:0.1 mol/mol/mol. Solubilisation of these model membranes was studied by static light scattering (nephelometry). Effective ratio Re (the amount of DDAO integrated into the bilayer to the amount of lipid) at different steps of the solubilisation process was determined. The molar partition coefficient of DDAO was calculated – in case of the POPE-POPG membrane, Kp = 5,300 ± 400, for the POPE-POPG-TOCL membrane, Kp = 6,500 ± 500.Želinská K.Gallová J.Sciendoarticlen,n-dimethyl-1-dodecanamine-n-oxidesolubilisationbacterial model membranescardiolipinTherapeutics. PharmacologyRM1-950ENEuropean Pharmaceutical Journal, Vol 68, Iss 1, Pp 17-23 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	n,n-dimethyl-1-dodecanamine-n-oxide solubilisation bacterial model membranes cardiolipin Therapeutics. Pharmacology RM1-950
spellingShingle	n,n-dimethyl-1-dodecanamine-n-oxide solubilisation bacterial model membranes cardiolipin Therapeutics. Pharmacology RM1-950 Želinská K. Gallová J. Study of the solubilisation process of bacterial model membranes induced by DDAO
description	Solubilisation of two bacterial model membranes induced by N,N-dimethyl-1-dodecanamine-N-oxide (DDAO) was studied. The first model membrane consisted of a mixture of palmitoyloleoylphosphatidylethanolamine (POPE) and palmitoyloleoylphosphatidylglycerol (POPG) in a molar ratio 0.6:0.4 mol/mol, and a second model membrane was enriched with tetraoleoylcardiolipin (TOCL) with a composition POPE-POPG-TOCL = 0.67:0.23:0.1 mol/mol/mol. Solubilisation of these model membranes was studied by static light scattering (nephelometry). Effective ratio Re (the amount of DDAO integrated into the bilayer to the amount of lipid) at different steps of the solubilisation process was determined. The molar partition coefficient of DDAO was calculated – in case of the POPE-POPG membrane, Kp = 5,300 ± 400, for the POPE-POPG-TOCL membrane, Kp = 6,500 ± 500.
format	article
author	Želinská K. Gallová J.
author_facet	Želinská K. Gallová J.
author_sort	Želinská K.
title	Study of the solubilisation process of bacterial model membranes induced by DDAO
title_short	Study of the solubilisation process of bacterial model membranes induced by DDAO
title_full	Study of the solubilisation process of bacterial model membranes induced by DDAO
title_fullStr	Study of the solubilisation process of bacterial model membranes induced by DDAO
title_full_unstemmed	Study of the solubilisation process of bacterial model membranes induced by DDAO
title_sort	study of the solubilisation process of bacterial model membranes induced by ddao
publisher	Sciendo
publishDate	2021
url	https://doaj.org/article/11330af00ecb4e9893411ecf3ca9d8e6
work_keys_str_mv	AT zelinskak studyofthesolubilisationprocessofbacterialmodelmembranesinducedbyddao AT gallovaj studyofthesolubilisationprocessofbacterialmodelmembranesinducedbyddao
_version_	1718371401978609664

Study of the solubilisation process of bacterial model membranes induced by DDAO

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