Extended latanoprost release from commercial contact lenses: in vitro studies using corneal models.

In this study, we compared, for the first time, the release of a 432 kDa prostaglandin F2a analogue drug, Latanoprost, from commercially available contact lenses using in vitro models with corneal epithelial cells. Conventional polyHEMA-based and silicone hydrogel soft contact lenses were soaked in...

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Autores principales:	Saman Mohammadi, Lyndon Jones, Maud Gorbet
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/113c582ef1694636a71f98596e53bc52
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spelling	oai:doaj.org-article:113c582ef1694636a71f98596e53bc522021-11-25T06:01:10ZExtended latanoprost release from commercial contact lenses: in vitro studies using corneal models.1932-620310.1371/journal.pone.0106653https://doaj.org/article/113c582ef1694636a71f98596e53bc522014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0106653https://doaj.org/toc/1932-6203In this study, we compared, for the first time, the release of a 432 kDa prostaglandin F2a analogue drug, Latanoprost, from commercially available contact lenses using in vitro models with corneal epithelial cells. Conventional polyHEMA-based and silicone hydrogel soft contact lenses were soaked in drug solution (131 μg = ml solution in phosphate buffered saline). The drug release from the contact lens material and its diffusion through three in vitro models was studied. The three in vitro models consisted of a polyethylene terephthalate (PET) membrane without corneal epithelial cells, a PET membrane with a monolayer of human corneal epithelial cells (HCEC), and a PET membrane with stratified HCEC. In the cell-based in vitro corneal epithelium models, a zero order release was obtained with the silicone hydrogel materials (linear for the duration of the experiment) whereby, after 48 hours, between 4 to 6 μg of latanoprost (an amount well within the range of the prescribed daily dose for glaucoma patients) was released. In the absence of cells, a significantly lower amount of drug, between 0.3 to 0.5 μg, was released, (p <0:001). The difference observed in release from the hydrogel lens materials in the presence and absence of cells emphasizes the importance of using an in vitro corneal model that is more representative of the physiological conditions in the eye to more adequately characterize ophthalmic drug delivery materials. Our results demonstrate how in vitro models with corneal epithelial cells may allow better prediction of in vivo release. It also highlights the potential of drug-soaked silicone hydrogel contact lens materials for drug delivery purposes.Saman MohammadiLyndon JonesMaud GorbetPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e106653 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Saman Mohammadi Lyndon Jones Maud Gorbet Extended latanoprost release from commercial contact lenses: in vitro studies using corneal models.
description	In this study, we compared, for the first time, the release of a 432 kDa prostaglandin F2a analogue drug, Latanoprost, from commercially available contact lenses using in vitro models with corneal epithelial cells. Conventional polyHEMA-based and silicone hydrogel soft contact lenses were soaked in drug solution (131 μg = ml solution in phosphate buffered saline). The drug release from the contact lens material and its diffusion through three in vitro models was studied. The three in vitro models consisted of a polyethylene terephthalate (PET) membrane without corneal epithelial cells, a PET membrane with a monolayer of human corneal epithelial cells (HCEC), and a PET membrane with stratified HCEC. In the cell-based in vitro corneal epithelium models, a zero order release was obtained with the silicone hydrogel materials (linear for the duration of the experiment) whereby, after 48 hours, between 4 to 6 μg of latanoprost (an amount well within the range of the prescribed daily dose for glaucoma patients) was released. In the absence of cells, a significantly lower amount of drug, between 0.3 to 0.5 μg, was released, (p <0:001). The difference observed in release from the hydrogel lens materials in the presence and absence of cells emphasizes the importance of using an in vitro corneal model that is more representative of the physiological conditions in the eye to more adequately characterize ophthalmic drug delivery materials. Our results demonstrate how in vitro models with corneal epithelial cells may allow better prediction of in vivo release. It also highlights the potential of drug-soaked silicone hydrogel contact lens materials for drug delivery purposes.
format	article
author	Saman Mohammadi Lyndon Jones Maud Gorbet
author_facet	Saman Mohammadi Lyndon Jones Maud Gorbet
author_sort	Saman Mohammadi
title	Extended latanoprost release from commercial contact lenses: in vitro studies using corneal models.
title_short	Extended latanoprost release from commercial contact lenses: in vitro studies using corneal models.
title_full	Extended latanoprost release from commercial contact lenses: in vitro studies using corneal models.
title_fullStr	Extended latanoprost release from commercial contact lenses: in vitro studies using corneal models.
title_full_unstemmed	Extended latanoprost release from commercial contact lenses: in vitro studies using corneal models.
title_sort	extended latanoprost release from commercial contact lenses: in vitro studies using corneal models.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/113c582ef1694636a71f98596e53bc52
work_keys_str_mv	AT samanmohammadi extendedlatanoprostreleasefromcommercialcontactlensesinvitrostudiesusingcornealmodels AT lyndonjones extendedlatanoprostreleasefromcommercialcontactlensesinvitrostudiesusingcornealmodels AT maudgorbet extendedlatanoprostreleasefromcommercialcontactlensesinvitrostudiesusingcornealmodels
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Extended latanoprost release from commercial contact lenses: in vitro studies using corneal models.

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