Integrated Datasets of Proteomic and Metabolomic Biomarkers to Predict Its Impacts on Comorbidities of Type 2 Diabetes Mellitus

Amrita K Cheema,1 Prabhjit Kaur,1 Amina Fadel,2 Noura Younes,3 Mahmoud Zirie,4 Nasser M Rizk2,5 1Department of Oncology, Lombardi Comprehensive Cancer Center at Georgetown University Medical Center, Washington, DC, USA; 2Biomedical Sciences Department, College of Health Sciences and Biomedical Resea...

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Autores principales:	Cheema AK, Kaur P, Fadel A, Younes N, Zirie M, Rizk NM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	type 2 diabetes mellitus pathway analysis regulators biomarkers disorders bioinformatics Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/113f03c14d3041c29cee9687f2d39e47
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spelling	oai:doaj.org-article:113f03c14d3041c29cee9687f2d39e472021-12-02T10:16:20ZIntegrated Datasets of Proteomic and Metabolomic Biomarkers to Predict Its Impacts on Comorbidities of Type 2 Diabetes Mellitus1178-7007https://doaj.org/article/113f03c14d3041c29cee9687f2d39e472020-07-01T00:00:00Zhttps://www.dovepress.com/integrated-datasets-of-proteomic-and-metabolomic-biomarkers-to-predict-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Amrita K Cheema,1 Prabhjit Kaur,1 Amina Fadel,2 Noura Younes,3 Mahmoud Zirie,4 Nasser M Rizk2,5 1Department of Oncology, Lombardi Comprehensive Cancer Center at Georgetown University Medical Center, Washington, DC, USA; 2Biomedical Sciences Department, College of Health Sciences and Biomedical Research Center, QU Health, Qatar University, Doha, Qatar; 3Clinical Chemistry Lab, Hamad Medical Corporation, Doha, Qatar; 4Endocrine Department, Hammad Medical Corporation, Doha, Qatar; 5Physiology Department, Mansoura Faculty of Medicine, Mansoura, EgyptCorrespondence: Nasser M RizkBiomedical Sciences Department, College of Health Sciences, Biomedical Research Center, QU Health, Qatar University, Doha, QatarEmail nassrizk@qu.edu.qaObjective: The objective of the current study is to accomplish a relative exploration of the biological roles of differentially dysregulated genes (DRGs) in type 2 diabetes mellitus (T2DM). The study aimed to determine the impact of these DRGs on the biological pathways and networks that are related to the associated disorders and complications in T2DM and to predict its role as prospective biomarkers.Methods: Datasets obtained from metabolomic and proteomic profiling were used for investigation of the differential expression of the genes. A subset of DRGs was integrated into IPA software to explore its biological pathways, related diseases, and their regulation in T2DM. Upon entry into the IPA, only 94 of the DRGs were recognizable, mapped, and matched within the database.Results: The study identified networks that explore the dysregulation of several functions; cell components such as degranulation of cells; molecular transport process and metabolism of cellular proteins; and inflammatory responses. Top disorders associated with DRGs in T2DM are related to organ injuries such as renal damage, connective tissue disorders, and acute inflammatory disorders. Upstream regulator analysis predicted the role of several transcription factors of interest, such as STAT3 and HIF alpha, as well as many kinases such as JAK kinases, which affects the gene expression of the dataset in T2DM. Interleukin 6 (IL6) is the top regulator of the DRGs, followed by leptin (LEP). Monitoring the dysregulation of the coupled expression of the following biomarkers (TNF, IL6, LEP, AGT, APOE, F2, SPP1, and INS) highlights that they could be used as potential prognostic biomarkers.Conclusion: The integration of data obtained by advanced metabolomic and proteomic technologies has made it probable to advantage in understanding the role of these biomarkers in the identification of significant biological processes, pathways, and regulators that are associated with T2DM and its comorbidities.Keywords: type 2 diabetes mellitus, pathway analysis, regulators, biomarkers, disorders, bioinformaticsCheema AKKaur PFadel AYounes NZirie MRizk NMDove Medical Pressarticletype 2 diabetes mellitus pathway analysis regulators biomarkers disorders bioinformaticsSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 2409-2431 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	type 2 diabetes mellitus pathway analysis regulators biomarkers disorders bioinformatics Specialties of internal medicine RC581-951
spellingShingle	type 2 diabetes mellitus pathway analysis regulators biomarkers disorders bioinformatics Specialties of internal medicine RC581-951 Cheema AK Kaur P Fadel A Younes N Zirie M Rizk NM Integrated Datasets of Proteomic and Metabolomic Biomarkers to Predict Its Impacts on Comorbidities of Type 2 Diabetes Mellitus
description	Amrita K Cheema,1 Prabhjit Kaur,1 Amina Fadel,2 Noura Younes,3 Mahmoud Zirie,4 Nasser M Rizk2,5 1Department of Oncology, Lombardi Comprehensive Cancer Center at Georgetown University Medical Center, Washington, DC, USA; 2Biomedical Sciences Department, College of Health Sciences and Biomedical Research Center, QU Health, Qatar University, Doha, Qatar; 3Clinical Chemistry Lab, Hamad Medical Corporation, Doha, Qatar; 4Endocrine Department, Hammad Medical Corporation, Doha, Qatar; 5Physiology Department, Mansoura Faculty of Medicine, Mansoura, EgyptCorrespondence: Nasser M RizkBiomedical Sciences Department, College of Health Sciences, Biomedical Research Center, QU Health, Qatar University, Doha, QatarEmail nassrizk@qu.edu.qaObjective: The objective of the current study is to accomplish a relative exploration of the biological roles of differentially dysregulated genes (DRGs) in type 2 diabetes mellitus (T2DM). The study aimed to determine the impact of these DRGs on the biological pathways and networks that are related to the associated disorders and complications in T2DM and to predict its role as prospective biomarkers.Methods: Datasets obtained from metabolomic and proteomic profiling were used for investigation of the differential expression of the genes. A subset of DRGs was integrated into IPA software to explore its biological pathways, related diseases, and their regulation in T2DM. Upon entry into the IPA, only 94 of the DRGs were recognizable, mapped, and matched within the database.Results: The study identified networks that explore the dysregulation of several functions; cell components such as degranulation of cells; molecular transport process and metabolism of cellular proteins; and inflammatory responses. Top disorders associated with DRGs in T2DM are related to organ injuries such as renal damage, connective tissue disorders, and acute inflammatory disorders. Upstream regulator analysis predicted the role of several transcription factors of interest, such as STAT3 and HIF alpha, as well as many kinases such as JAK kinases, which affects the gene expression of the dataset in T2DM. Interleukin 6 (IL6) is the top regulator of the DRGs, followed by leptin (LEP). Monitoring the dysregulation of the coupled expression of the following biomarkers (TNF, IL6, LEP, AGT, APOE, F2, SPP1, and INS) highlights that they could be used as potential prognostic biomarkers.Conclusion: The integration of data obtained by advanced metabolomic and proteomic technologies has made it probable to advantage in understanding the role of these biomarkers in the identification of significant biological processes, pathways, and regulators that are associated with T2DM and its comorbidities.Keywords: type 2 diabetes mellitus, pathway analysis, regulators, biomarkers, disorders, bioinformatics
format	article
author	Cheema AK Kaur P Fadel A Younes N Zirie M Rizk NM
author_facet	Cheema AK Kaur P Fadel A Younes N Zirie M Rizk NM
author_sort	Cheema AK
title	Integrated Datasets of Proteomic and Metabolomic Biomarkers to Predict Its Impacts on Comorbidities of Type 2 Diabetes Mellitus
title_short	Integrated Datasets of Proteomic and Metabolomic Biomarkers to Predict Its Impacts on Comorbidities of Type 2 Diabetes Mellitus
title_full	Integrated Datasets of Proteomic and Metabolomic Biomarkers to Predict Its Impacts on Comorbidities of Type 2 Diabetes Mellitus
title_fullStr	Integrated Datasets of Proteomic and Metabolomic Biomarkers to Predict Its Impacts on Comorbidities of Type 2 Diabetes Mellitus
title_full_unstemmed	Integrated Datasets of Proteomic and Metabolomic Biomarkers to Predict Its Impacts on Comorbidities of Type 2 Diabetes Mellitus
title_sort	integrated datasets of proteomic and metabolomic biomarkers to predict its impacts on comorbidities of type 2 diabetes mellitus
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/113f03c14d3041c29cee9687f2d39e47
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Integrated Datasets of Proteomic and Metabolomic Biomarkers to Predict Its Impacts on Comorbidities of Type 2 Diabetes Mellitus

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