Arabidopsis thaliana glyoxalase 2-1 is required during abiotic stress but is not essential under normal plant growth.

The glyoxalase pathway, which consists of the two enzymes, GLYOXALASE 1 (GLX 1) (E.C.: 4.4.1.5) and 2 (E.C.3.1.2.6), has a vital role in chemical detoxification. In Arabidopsis thaliana there are at least four different isoforms of glyoxalase 2, two of which, GLX2-1 and GLX2-4 have not been characte...

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Autores principales:	Sriram Devanathan, Alexander Erban, Rodolfo Perez-Torres, Joachim Kopka, Christopher A Makaroff
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/114f9ba08ecf4566a3313cac49594483
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spelling	oai:doaj.org-article:114f9ba08ecf4566a3313cac495944832021-11-18T08:21:42ZArabidopsis thaliana glyoxalase 2-1 is required during abiotic stress but is not essential under normal plant growth.1932-620310.1371/journal.pone.0095971https://doaj.org/article/114f9ba08ecf4566a3313cac495944832014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24760003/?tool=EBIhttps://doaj.org/toc/1932-6203The glyoxalase pathway, which consists of the two enzymes, GLYOXALASE 1 (GLX 1) (E.C.: 4.4.1.5) and 2 (E.C.3.1.2.6), has a vital role in chemical detoxification. In Arabidopsis thaliana there are at least four different isoforms of glyoxalase 2, two of which, GLX2-1 and GLX2-4 have not been characterized in detail. Here, the functional role of Arabidopsis thaliana GLX2-1 is investigated. Glx2-1 loss-of-function mutants and plants that constitutively over-express GLX2-1 resemble wild-type plants under normal growth conditions. Insilico analysis of publicly available microarray datasets with ATTEDII, Mapman and Genevestigator indicate potential role(s) in stress response and acclimation. Results presented here demonstrate that GLX2-1 gene expression is up-regulated in wild type Arabidopsis thaliana by salt and anoxia stress, and by excess L-Threonine. Additionally, a mutation in GLX2-1 inhibits growth and survival during abiotic stresses. Metabolic profiling studies show alterations in the levels of sugars and amino acids during threonine stress in the plants. Elevated levels of polyamines, which are known stress markers, are also observed. Overall our results suggest that Arabidopsis thaliana GLX2-1 is not essential during normal plant life, but is required during specific stress conditions.Sriram DevanathanAlexander ErbanRodolfo Perez-TorresJoachim KopkaChristopher A MakaroffPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e95971 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sriram Devanathan Alexander Erban Rodolfo Perez-Torres Joachim Kopka Christopher A Makaroff Arabidopsis thaliana glyoxalase 2-1 is required during abiotic stress but is not essential under normal plant growth.
description	The glyoxalase pathway, which consists of the two enzymes, GLYOXALASE 1 (GLX 1) (E.C.: 4.4.1.5) and 2 (E.C.3.1.2.6), has a vital role in chemical detoxification. In Arabidopsis thaliana there are at least four different isoforms of glyoxalase 2, two of which, GLX2-1 and GLX2-4 have not been characterized in detail. Here, the functional role of Arabidopsis thaliana GLX2-1 is investigated. Glx2-1 loss-of-function mutants and plants that constitutively over-express GLX2-1 resemble wild-type plants under normal growth conditions. Insilico analysis of publicly available microarray datasets with ATTEDII, Mapman and Genevestigator indicate potential role(s) in stress response and acclimation. Results presented here demonstrate that GLX2-1 gene expression is up-regulated in wild type Arabidopsis thaliana by salt and anoxia stress, and by excess L-Threonine. Additionally, a mutation in GLX2-1 inhibits growth and survival during abiotic stresses. Metabolic profiling studies show alterations in the levels of sugars and amino acids during threonine stress in the plants. Elevated levels of polyamines, which are known stress markers, are also observed. Overall our results suggest that Arabidopsis thaliana GLX2-1 is not essential during normal plant life, but is required during specific stress conditions.
format	article
author	Sriram Devanathan Alexander Erban Rodolfo Perez-Torres Joachim Kopka Christopher A Makaroff
author_facet	Sriram Devanathan Alexander Erban Rodolfo Perez-Torres Joachim Kopka Christopher A Makaroff
author_sort	Sriram Devanathan
title	Arabidopsis thaliana glyoxalase 2-1 is required during abiotic stress but is not essential under normal plant growth.
title_short	Arabidopsis thaliana glyoxalase 2-1 is required during abiotic stress but is not essential under normal plant growth.
title_full	Arabidopsis thaliana glyoxalase 2-1 is required during abiotic stress but is not essential under normal plant growth.
title_fullStr	Arabidopsis thaliana glyoxalase 2-1 is required during abiotic stress but is not essential under normal plant growth.
title_full_unstemmed	Arabidopsis thaliana glyoxalase 2-1 is required during abiotic stress but is not essential under normal plant growth.
title_sort	arabidopsis thaliana glyoxalase 2-1 is required during abiotic stress but is not essential under normal plant growth.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/114f9ba08ecf4566a3313cac49594483
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Arabidopsis thaliana glyoxalase 2-1 is required during abiotic stress but is not essential under normal plant growth.

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