The neuroprotective effect of hesperidin in NMDA-induced retinal injury acts by suppressing oxidative stress and excessive calpain activation

Abstract We found that hesperidin, a plant-derived bioflavonoid, may be a candidate agent for neuroprotective treatment in the retina, after screening 41 materials for anti-oxidative properties in a primary retinal cell culture under oxidative stress. We found that the intravitreal injection of hesp...

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Autores principales:	Shigeto Maekawa, Kota Sato, Kosuke Fujita, Reiko Daigaku, Hiroshi Tawarayama, Namie Murayama, Satoru Moritoh, Takeshi Yabana, Yukihiro Shiga, Kazuko Omodaka, Kazuichi Maruyama, Koji M. Nishiguchi, Toru Nakazawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1152ef1d7d4948ffb2f2facd7cfa5e70
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spelling	oai:doaj.org-article:1152ef1d7d4948ffb2f2facd7cfa5e702021-12-02T11:52:23ZThe neuroprotective effect of hesperidin in NMDA-induced retinal injury acts by suppressing oxidative stress and excessive calpain activation10.1038/s41598-017-06969-42045-2322https://doaj.org/article/1152ef1d7d4948ffb2f2facd7cfa5e702017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06969-4https://doaj.org/toc/2045-2322Abstract We found that hesperidin, a plant-derived bioflavonoid, may be a candidate agent for neuroprotective treatment in the retina, after screening 41 materials for anti-oxidative properties in a primary retinal cell culture under oxidative stress. We found that the intravitreal injection of hesperidin in mice prevented reductions in markers of the retinal ganglion cells (RGCs) and RGC death after N-methyl-D-aspartate (NMDA)-induced excitotoxicity. Hesperidin treatment also reduced calpain activation, reactive oxygen species generation and TNF-α gene expression. Finally, hesperidin treatment improved electrophysiological function, measured with visual evoked potential, and visual function, measured with optomotry. Thus, we found that hesperidin suppressed a number of cytotoxic factors associated with NMDA-induced cell death signaling, such as oxidative stress, over-activation of calpain, and inflammation, thereby protecting the RGCs in mice. Therefore, hesperidin may have potential as a therapeutic supplement for protecting the retina against the damage associated with excitotoxic injury, such as occurs in glaucoma and diabetic retinopathy.Shigeto MaekawaKota SatoKosuke FujitaReiko DaigakuHiroshi TawarayamaNamie MurayamaSatoru MoritohTakeshi YabanaYukihiro ShigaKazuko OmodakaKazuichi MaruyamaKoji M. NishiguchiToru NakazawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shigeto Maekawa Kota Sato Kosuke Fujita Reiko Daigaku Hiroshi Tawarayama Namie Murayama Satoru Moritoh Takeshi Yabana Yukihiro Shiga Kazuko Omodaka Kazuichi Maruyama Koji M. Nishiguchi Toru Nakazawa The neuroprotective effect of hesperidin in NMDA-induced retinal injury acts by suppressing oxidative stress and excessive calpain activation
description	Abstract We found that hesperidin, a plant-derived bioflavonoid, may be a candidate agent for neuroprotective treatment in the retina, after screening 41 materials for anti-oxidative properties in a primary retinal cell culture under oxidative stress. We found that the intravitreal injection of hesperidin in mice prevented reductions in markers of the retinal ganglion cells (RGCs) and RGC death after N-methyl-D-aspartate (NMDA)-induced excitotoxicity. Hesperidin treatment also reduced calpain activation, reactive oxygen species generation and TNF-α gene expression. Finally, hesperidin treatment improved electrophysiological function, measured with visual evoked potential, and visual function, measured with optomotry. Thus, we found that hesperidin suppressed a number of cytotoxic factors associated with NMDA-induced cell death signaling, such as oxidative stress, over-activation of calpain, and inflammation, thereby protecting the RGCs in mice. Therefore, hesperidin may have potential as a therapeutic supplement for protecting the retina against the damage associated with excitotoxic injury, such as occurs in glaucoma and diabetic retinopathy.
format	article
author	Shigeto Maekawa Kota Sato Kosuke Fujita Reiko Daigaku Hiroshi Tawarayama Namie Murayama Satoru Moritoh Takeshi Yabana Yukihiro Shiga Kazuko Omodaka Kazuichi Maruyama Koji M. Nishiguchi Toru Nakazawa
author_facet	Shigeto Maekawa Kota Sato Kosuke Fujita Reiko Daigaku Hiroshi Tawarayama Namie Murayama Satoru Moritoh Takeshi Yabana Yukihiro Shiga Kazuko Omodaka Kazuichi Maruyama Koji M. Nishiguchi Toru Nakazawa
author_sort	Shigeto Maekawa
title	The neuroprotective effect of hesperidin in NMDA-induced retinal injury acts by suppressing oxidative stress and excessive calpain activation
title_short	The neuroprotective effect of hesperidin in NMDA-induced retinal injury acts by suppressing oxidative stress and excessive calpain activation
title_full	The neuroprotective effect of hesperidin in NMDA-induced retinal injury acts by suppressing oxidative stress and excessive calpain activation
title_fullStr	The neuroprotective effect of hesperidin in NMDA-induced retinal injury acts by suppressing oxidative stress and excessive calpain activation
title_full_unstemmed	The neuroprotective effect of hesperidin in NMDA-induced retinal injury acts by suppressing oxidative stress and excessive calpain activation
title_sort	neuroprotective effect of hesperidin in nmda-induced retinal injury acts by suppressing oxidative stress and excessive calpain activation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/1152ef1d7d4948ffb2f2facd7cfa5e70
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The neuroprotective effect of hesperidin in NMDA-induced retinal injury acts by suppressing oxidative stress and excessive calpain activation

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