DAPL1 is a novel regulator of testosterone production in Leydig cells of mouse testis

Abstract Leydig cells in the testes produce testosterone in the presence of gonadotropins. Therefore, male testosterone levels must oscillate within a healthy spectrum, given that elevated testosterone levels augment the risk of cardiovascular disorders. We observed that the expression of death-asso...

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Autores principales:	Hong-bin Chen, Jorge Carlos Pineda Garcia, Shinako Arizono, Tomoki Takeda, Ren-shi Li, Yukiko Hattori, Hiroe Sano, Yuu Miyauchi, Yuko Hirota, Yoshitaka Tanaka, Yuji Ishii
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1153c0cb3cce452b981c9a12bfbdd875
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spelling	oai:doaj.org-article:1153c0cb3cce452b981c9a12bfbdd8752021-12-02T15:15:35ZDAPL1 is a novel regulator of testosterone production in Leydig cells of mouse testis10.1038/s41598-021-97961-62045-2322https://doaj.org/article/1153c0cb3cce452b981c9a12bfbdd8752021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97961-6https://doaj.org/toc/2045-2322Abstract Leydig cells in the testes produce testosterone in the presence of gonadotropins. Therefore, male testosterone levels must oscillate within a healthy spectrum, given that elevated testosterone levels augment the risk of cardiovascular disorders. We observed that the expression of death-associated protein-like 1 (DAPL1), which is involved in the early stages of epithelial differentiation and apoptosis, is considerably higher in the testes of sexually mature mice than in other tissues. Accordingly, Dapl1-null mice were constructed to evaluate this variation. Notably, in these mice, the testicular levels of steroidogenic acute regulatory protein (StAR) and serum testosterone levels were significantly elevated on postnatal day 49. The findings were confirmed in vitro using I-10 mouse testis-derived tumor cells. The in vivo and in vitro data revealed the DAPL1-regulated the expression of StAR involving altered transcription of critical proteins in the protein kinase A and CREB/CREM pathways in Leydig cells. The collective findings implicate DAPL1 as an important factor for steroidogenesis regulation, and DAPL1 deregulation may be related to high endogenous levels of testosterone.Hong-bin ChenJorge Carlos Pineda GarciaShinako ArizonoTomoki TakedaRen-shi LiYukiko HattoriHiroe SanoYuu MiyauchiYuko HirotaYoshitaka TanakaYuji IshiiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hong-bin Chen Jorge Carlos Pineda Garcia Shinako Arizono Tomoki Takeda Ren-shi Li Yukiko Hattori Hiroe Sano Yuu Miyauchi Yuko Hirota Yoshitaka Tanaka Yuji Ishii DAPL1 is a novel regulator of testosterone production in Leydig cells of mouse testis
description	Abstract Leydig cells in the testes produce testosterone in the presence of gonadotropins. Therefore, male testosterone levels must oscillate within a healthy spectrum, given that elevated testosterone levels augment the risk of cardiovascular disorders. We observed that the expression of death-associated protein-like 1 (DAPL1), which is involved in the early stages of epithelial differentiation and apoptosis, is considerably higher in the testes of sexually mature mice than in other tissues. Accordingly, Dapl1-null mice were constructed to evaluate this variation. Notably, in these mice, the testicular levels of steroidogenic acute regulatory protein (StAR) and serum testosterone levels were significantly elevated on postnatal day 49. The findings were confirmed in vitro using I-10 mouse testis-derived tumor cells. The in vivo and in vitro data revealed the DAPL1-regulated the expression of StAR involving altered transcription of critical proteins in the protein kinase A and CREB/CREM pathways in Leydig cells. The collective findings implicate DAPL1 as an important factor for steroidogenesis regulation, and DAPL1 deregulation may be related to high endogenous levels of testosterone.
format	article
author	Hong-bin Chen Jorge Carlos Pineda Garcia Shinako Arizono Tomoki Takeda Ren-shi Li Yukiko Hattori Hiroe Sano Yuu Miyauchi Yuko Hirota Yoshitaka Tanaka Yuji Ishii
author_facet	Hong-bin Chen Jorge Carlos Pineda Garcia Shinako Arizono Tomoki Takeda Ren-shi Li Yukiko Hattori Hiroe Sano Yuu Miyauchi Yuko Hirota Yoshitaka Tanaka Yuji Ishii
author_sort	Hong-bin Chen
title	DAPL1 is a novel regulator of testosterone production in Leydig cells of mouse testis
title_short	DAPL1 is a novel regulator of testosterone production in Leydig cells of mouse testis
title_full	DAPL1 is a novel regulator of testosterone production in Leydig cells of mouse testis
title_fullStr	DAPL1 is a novel regulator of testosterone production in Leydig cells of mouse testis
title_full_unstemmed	DAPL1 is a novel regulator of testosterone production in Leydig cells of mouse testis
title_sort	dapl1 is a novel regulator of testosterone production in leydig cells of mouse testis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1153c0cb3cce452b981c9a12bfbdd875
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DAPL1 is a novel regulator of testosterone production in Leydig cells of mouse testis

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