Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas

Abstract To better understand the pathogenesis of nasal polyps (NPs) and sinonasal inverted papillomas (SIPs), we aimed to establish cell lines from fresh tissues of NPs and SIPs and characterize them. Primary cell cultures were obtained from two NP tissues (NP2 and NP3) and one SIP tissue (IP4). Al...

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Autores principales:	Thawaree Nukpook, Tipaya Ekalaksananan, Tohru Kiyono, Pornthep Kasemsiri, Watchareporn Teeramatwanich, Patravoot Vatanasapt, Surachat Chaiwiriyakul, Piti Ungarreevittaya, Jureeporn Kampan, Kanha Muisuk, Chamsai Pientong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1153f44d7bfb4d2d8e887b45dd74cd6a
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spelling	oai:doaj.org-article:1153f44d7bfb4d2d8e887b45dd74cd6a2021-12-02T19:02:39ZEstablishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas10.1038/s41598-021-96444-y2045-2322https://doaj.org/article/1153f44d7bfb4d2d8e887b45dd74cd6a2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96444-yhttps://doaj.org/toc/2045-2322Abstract To better understand the pathogenesis of nasal polyps (NPs) and sinonasal inverted papillomas (SIPs), we aimed to establish cell lines from fresh tissues of NPs and SIPs and characterize them. Primary cell cultures were obtained from two NP tissues (NP2 and NP3) and one SIP tissue (IP4). All the cells were polygonal in shape, expressed cytokeratin 14, and had normal diploid chromosome status. HPV58 DNA was detected in NP3. To obtain immortal primary cells, NP2 and IP4 cells were transduced with a combination of mutant CDK4, cyclinD1 and TERT. These cells were thereafter named NP2/K4DT and IP4/K4DT, respectively. HPV58-positive NP3 cells were transduced with TERT alone, the resulting cells named NP3/T. Phenotypic and genotypic identity of original tissues and derived cells was investigated. All the cell cultures with transgenes were confirmed to be derived from their parental cells and primary tumor tissues by analysis of short tandem repeats (STR) and maintained in vitro growth, genetic profiles and gene expression characteristics of the primary cells. These virtually immortalized cells, as well as the primary cells, have potential as in vitro models for studying the pathogenesis of NPs and SIPs and for preclinical study to develop new therapeutic agents.Thawaree NukpookTipaya EkalaksanananTohru KiyonoPornthep KasemsiriWatchareporn TeeramatwanichPatravoot VatanasaptSurachat ChaiwiriyakulPiti UngarreevittayaJureeporn KampanKanha MuisukChamsai PientongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thawaree Nukpook Tipaya Ekalaksananan Tohru Kiyono Pornthep Kasemsiri Watchareporn Teeramatwanich Patravoot Vatanasapt Surachat Chaiwiriyakul Piti Ungarreevittaya Jureeporn Kampan Kanha Muisuk Chamsai Pientong Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas
description	Abstract To better understand the pathogenesis of nasal polyps (NPs) and sinonasal inverted papillomas (SIPs), we aimed to establish cell lines from fresh tissues of NPs and SIPs and characterize them. Primary cell cultures were obtained from two NP tissues (NP2 and NP3) and one SIP tissue (IP4). All the cells were polygonal in shape, expressed cytokeratin 14, and had normal diploid chromosome status. HPV58 DNA was detected in NP3. To obtain immortal primary cells, NP2 and IP4 cells were transduced with a combination of mutant CDK4, cyclinD1 and TERT. These cells were thereafter named NP2/K4DT and IP4/K4DT, respectively. HPV58-positive NP3 cells were transduced with TERT alone, the resulting cells named NP3/T. Phenotypic and genotypic identity of original tissues and derived cells was investigated. All the cell cultures with transgenes were confirmed to be derived from their parental cells and primary tumor tissues by analysis of short tandem repeats (STR) and maintained in vitro growth, genetic profiles and gene expression characteristics of the primary cells. These virtually immortalized cells, as well as the primary cells, have potential as in vitro models for studying the pathogenesis of NPs and SIPs and for preclinical study to develop new therapeutic agents.
format	article
author	Thawaree Nukpook Tipaya Ekalaksananan Tohru Kiyono Pornthep Kasemsiri Watchareporn Teeramatwanich Patravoot Vatanasapt Surachat Chaiwiriyakul Piti Ungarreevittaya Jureeporn Kampan Kanha Muisuk Chamsai Pientong
author_facet	Thawaree Nukpook Tipaya Ekalaksananan Tohru Kiyono Pornthep Kasemsiri Watchareporn Teeramatwanich Patravoot Vatanasapt Surachat Chaiwiriyakul Piti Ungarreevittaya Jureeporn Kampan Kanha Muisuk Chamsai Pientong
author_sort	Thawaree Nukpook
title	Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas
title_short	Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas
title_full	Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas
title_fullStr	Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas
title_full_unstemmed	Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas
title_sort	establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1153f44d7bfb4d2d8e887b45dd74cd6a
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Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas

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