Cell damage produced by magnetic fluid hyperthermia on microglial BV2 cells

Abstract We present evidence on the effects of exogenous heating by water bath (WB) and magnetic hyperthermia (MHT) on a glial micro-tumor phantom. To this, magnetic nanoparticles (MNPs) of 30–40 nm were designed to obtain particle sizes for maximum heating efficiency. The specific power absorption...

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Autores principales:	M. Pilar Calatayud, Elisa Soler, Teobaldo E. Torres, Enrique Campos-Gonzalez, Concepción Junquera, M. Ricardo Ibarra, Gerardo F. Goya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/115a6fa674e9487ba4b2a8c91d63a5e8
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spelling	oai:doaj.org-article:115a6fa674e9487ba4b2a8c91d63a5e82021-12-02T16:06:59ZCell damage produced by magnetic fluid hyperthermia on microglial BV2 cells10.1038/s41598-017-09059-72045-2322https://doaj.org/article/115a6fa674e9487ba4b2a8c91d63a5e82017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09059-7https://doaj.org/toc/2045-2322Abstract We present evidence on the effects of exogenous heating by water bath (WB) and magnetic hyperthermia (MHT) on a glial micro-tumor phantom. To this, magnetic nanoparticles (MNPs) of 30–40 nm were designed to obtain particle sizes for maximum heating efficiency. The specific power absorption (SPA) values (f = 560 kHz, H = 23.9 kA/m) for as prepared colloids (533–605 W/g) dropped to 98–279 W/g in culture medium. The analysis of the intracellular MNPs distribution showed vesicle-trapped MNPs agglomerates spread along the cytoplasm, as well as large (~0.5–0.9 μm) clusters attached to the cell membrane. Immediately after WB and MHT (T = 46 °C for 30 min) the cell viability was ≈70% and, after 4.5 h, decreased to 20–25%, demonstrating that metabolic processes are involved in cell killing. The analysis of the cell structures after MHT revealed a significant damage of the cell membrane that is correlated to the location of MNPs clusters, while local cell damage were less noticeable after WB without MNPs. In spite of the similar thermal effects of WB and MHT on the cell viability, our results suggest that there is an additional mechanism of cell damage related to the presence of MNPs at the intracellular space.M. Pilar CalatayudElisa SolerTeobaldo E. TorresEnrique Campos-GonzalezConcepción JunqueraM. Ricardo IbarraGerardo F. GoyaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. Pilar Calatayud Elisa Soler Teobaldo E. Torres Enrique Campos-Gonzalez Concepción Junquera M. Ricardo Ibarra Gerardo F. Goya Cell damage produced by magnetic fluid hyperthermia on microglial BV2 cells
description	Abstract We present evidence on the effects of exogenous heating by water bath (WB) and magnetic hyperthermia (MHT) on a glial micro-tumor phantom. To this, magnetic nanoparticles (MNPs) of 30–40 nm were designed to obtain particle sizes for maximum heating efficiency. The specific power absorption (SPA) values (f = 560 kHz, H = 23.9 kA/m) for as prepared colloids (533–605 W/g) dropped to 98–279 W/g in culture medium. The analysis of the intracellular MNPs distribution showed vesicle-trapped MNPs agglomerates spread along the cytoplasm, as well as large (~0.5–0.9 μm) clusters attached to the cell membrane. Immediately after WB and MHT (T = 46 °C for 30 min) the cell viability was ≈70% and, after 4.5 h, decreased to 20–25%, demonstrating that metabolic processes are involved in cell killing. The analysis of the cell structures after MHT revealed a significant damage of the cell membrane that is correlated to the location of MNPs clusters, while local cell damage were less noticeable after WB without MNPs. In spite of the similar thermal effects of WB and MHT on the cell viability, our results suggest that there is an additional mechanism of cell damage related to the presence of MNPs at the intracellular space.
format	article
author	M. Pilar Calatayud Elisa Soler Teobaldo E. Torres Enrique Campos-Gonzalez Concepción Junquera M. Ricardo Ibarra Gerardo F. Goya
author_facet	M. Pilar Calatayud Elisa Soler Teobaldo E. Torres Enrique Campos-Gonzalez Concepción Junquera M. Ricardo Ibarra Gerardo F. Goya
author_sort	M. Pilar Calatayud
title	Cell damage produced by magnetic fluid hyperthermia on microglial BV2 cells
title_short	Cell damage produced by magnetic fluid hyperthermia on microglial BV2 cells
title_full	Cell damage produced by magnetic fluid hyperthermia on microglial BV2 cells
title_fullStr	Cell damage produced by magnetic fluid hyperthermia on microglial BV2 cells
title_full_unstemmed	Cell damage produced by magnetic fluid hyperthermia on microglial BV2 cells
title_sort	cell damage produced by magnetic fluid hyperthermia on microglial bv2 cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/115a6fa674e9487ba4b2a8c91d63a5e8
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Cell damage produced by magnetic fluid hyperthermia on microglial BV2 cells

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