Evolutionary genomics of APSE: a tailed phage that lysogenically converts the bacterium Hamiltonella defensa into a heritable protective symbiont of aphids

Abstract Background Most phages infect free-living bacteria but a few have been identified that infect heritable symbionts of insects or other eukaryotes. Heritable symbionts are usually specialized and isolated from other bacteria with little known about the origins of associated phages. Hamiltonel...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bret M. Boyd, Germain Chevignon, Vilas Patel, Kerry M. Oliver, Michael R. Strand
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Virus Bacteria Mutualism Aphid Parasitoid Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/11600e04f127422f826f3c54fef16d86
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:11600e04f127422f826f3c54fef16d86
record_format	dspace
spelling	oai:doaj.org-article:11600e04f127422f826f3c54fef16d862021-11-14T12:05:22ZEvolutionary genomics of APSE: a tailed phage that lysogenically converts the bacterium Hamiltonella defensa into a heritable protective symbiont of aphids10.1186/s12985-021-01685-y1743-422Xhttps://doaj.org/article/11600e04f127422f826f3c54fef16d862021-11-01T00:00:00Zhttps://doi.org/10.1186/s12985-021-01685-yhttps://doaj.org/toc/1743-422XAbstract Background Most phages infect free-living bacteria but a few have been identified that infect heritable symbionts of insects or other eukaryotes. Heritable symbionts are usually specialized and isolated from other bacteria with little known about the origins of associated phages. Hamiltonella defensa is a heritable bacterial symbiont of aphids that is usually infected by a tailed, double-stranded DNA phage named APSE. Methods We conducted comparative genomic and phylogenetic studies to determine how APSE is related to other phages and prophages. Results Each APSE genome was organized into four modules and two predicted functional units. Gene content and order were near-fully conserved in modules 1 and 2, which encode predicted DNA metabolism genes, and module 4, which encodes predicted virion assembly genes. Gene content of module 3, which contains predicted toxin, holin and lysozyme genes differed among haplotypes. Comparisons to other sequenced phages suggested APSE genomes are mosaics with modules 1 and 2 sharing similarities with Bordetella-Bcep-Xylostella fastidiosa-like podoviruses, module 4 sharing similarities with P22-like podoviruses, and module 3 sharing no similarities with known phages. Comparisons to other sequenced bacterial genomes identified APSE-like elements in other heritable insect symbionts (Arsenophonus spp.) and enteric bacteria in the family Morganellaceae. Conclusions APSEs are most closely related to phage elements in the genus Arsenophonus and other bacteria in the Morganellaceae.Bret M. BoydGermain ChevignonVilas PatelKerry M. OliverMichael R. StrandBMCarticleVirusBacteriaMutualismAphidParasitoidInfectious and parasitic diseasesRC109-216ENVirology Journal, Vol 18, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Virus Bacteria Mutualism Aphid Parasitoid Infectious and parasitic diseases RC109-216
spellingShingle	Virus Bacteria Mutualism Aphid Parasitoid Infectious and parasitic diseases RC109-216 Bret M. Boyd Germain Chevignon Vilas Patel Kerry M. Oliver Michael R. Strand Evolutionary genomics of APSE: a tailed phage that lysogenically converts the bacterium Hamiltonella defensa into a heritable protective symbiont of aphids
description	Abstract Background Most phages infect free-living bacteria but a few have been identified that infect heritable symbionts of insects or other eukaryotes. Heritable symbionts are usually specialized and isolated from other bacteria with little known about the origins of associated phages. Hamiltonella defensa is a heritable bacterial symbiont of aphids that is usually infected by a tailed, double-stranded DNA phage named APSE. Methods We conducted comparative genomic and phylogenetic studies to determine how APSE is related to other phages and prophages. Results Each APSE genome was organized into four modules and two predicted functional units. Gene content and order were near-fully conserved in modules 1 and 2, which encode predicted DNA metabolism genes, and module 4, which encodes predicted virion assembly genes. Gene content of module 3, which contains predicted toxin, holin and lysozyme genes differed among haplotypes. Comparisons to other sequenced phages suggested APSE genomes are mosaics with modules 1 and 2 sharing similarities with Bordetella-Bcep-Xylostella fastidiosa-like podoviruses, module 4 sharing similarities with P22-like podoviruses, and module 3 sharing no similarities with known phages. Comparisons to other sequenced bacterial genomes identified APSE-like elements in other heritable insect symbionts (Arsenophonus spp.) and enteric bacteria in the family Morganellaceae. Conclusions APSEs are most closely related to phage elements in the genus Arsenophonus and other bacteria in the Morganellaceae.
format	article
author	Bret M. Boyd Germain Chevignon Vilas Patel Kerry M. Oliver Michael R. Strand
author_facet	Bret M. Boyd Germain Chevignon Vilas Patel Kerry M. Oliver Michael R. Strand
author_sort	Bret M. Boyd
title	Evolutionary genomics of APSE: a tailed phage that lysogenically converts the bacterium Hamiltonella defensa into a heritable protective symbiont of aphids
title_short	Evolutionary genomics of APSE: a tailed phage that lysogenically converts the bacterium Hamiltonella defensa into a heritable protective symbiont of aphids
title_full	Evolutionary genomics of APSE: a tailed phage that lysogenically converts the bacterium Hamiltonella defensa into a heritable protective symbiont of aphids
title_fullStr	Evolutionary genomics of APSE: a tailed phage that lysogenically converts the bacterium Hamiltonella defensa into a heritable protective symbiont of aphids
title_full_unstemmed	Evolutionary genomics of APSE: a tailed phage that lysogenically converts the bacterium Hamiltonella defensa into a heritable protective symbiont of aphids
title_sort	evolutionary genomics of apse: a tailed phage that lysogenically converts the bacterium hamiltonella defensa into a heritable protective symbiont of aphids
publisher	BMC
publishDate	2021
url	https://doaj.org/article/11600e04f127422f826f3c54fef16d86
work_keys_str_mv	AT bretmboyd evolutionarygenomicsofapseatailedphagethatlysogenicallyconvertsthebacteriumhamiltonelladefensaintoaheritableprotectivesymbiontofaphids AT germainchevignon evolutionarygenomicsofapseatailedphagethatlysogenicallyconvertsthebacteriumhamiltonelladefensaintoaheritableprotectivesymbiontofaphids AT vilaspatel evolutionarygenomicsofapseatailedphagethatlysogenicallyconvertsthebacteriumhamiltonelladefensaintoaheritableprotectivesymbiontofaphids AT kerrymoliver evolutionarygenomicsofapseatailedphagethatlysogenicallyconvertsthebacteriumhamiltonelladefensaintoaheritableprotectivesymbiontofaphids AT michaelrstrand evolutionarygenomicsofapseatailedphagethatlysogenicallyconvertsthebacteriumhamiltonelladefensaintoaheritableprotectivesymbiontofaphids
_version_	1718429449659088896

Evolutionary genomics of APSE: a tailed phage that lysogenically converts the bacterium Hamiltonella defensa into a heritable protective symbiont of aphids

Ejemplares similares