Intraocular and systemic levels of vascular endothelial growth factor in advanced cases of retinopathy of prematurity

Raul Velez-Montoya1, Carmen Clapp2, Jose Carlos Rivera2, Gerardo Garcia-Aguirre1, Virgilio Morales-Cant&oacute;n1, Jans Fromow-Guerra1, Jose Luis Guerrero-Naranjo1, Hugo Quiroz-Mercado31Retina Department Asociaci&oacute;n para Evitar la Ceguera en M&eacute;xico IAP, M&amp...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Raul Velez-Montoya, Carmen Clapp, Jose Carlos Rivera, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/1164358f389747d0bea7c10b6a09ce59
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1164358f389747d0bea7c10b6a09ce59
record_format	dspace
spelling	oai:doaj.org-article:1164358f389747d0bea7c10b6a09ce592021-12-02T00:59:10ZIntraocular and systemic levels of vascular endothelial growth factor in advanced cases of retinopathy of prematurity1177-54671177-5483https://doaj.org/article/1164358f389747d0bea7c10b6a09ce592010-08-01T00:00:00Zhttp://www.dovepress.com/intraocular-and-systemic-levels-of-vascular-endothelial-growth-factor--a5105https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Raul Velez-Montoya1, Carmen Clapp2, Jose Carlos Rivera2, Gerardo Garcia-Aguirre1, Virgilio Morales-Cant&oacute;n1, Jans Fromow-Guerra1, Jose Luis Guerrero-Naranjo1, Hugo Quiroz-Mercado31Retina Department Asociaci&oacute;n para Evitar la Ceguera en M&eacute;xico IAP, M&eacute;xico City, M&eacute;xico; 2Department of Cellular and Molecular Neurobiology, Universidad Nacional Aut&oacute;noma de M&eacute;xico, Quer&eacute;taro, M&eacute;xico; 3Department of Ophthalmology, Denver Health Medical Center, University of Colorado School of Medicine, Colorado, USAPurpose: To measure vitreous, aqueous, subretinal fluid and plasma levels of vascular &shy;endothelial growth factor in late stages of retinopathy of prematurity.Methods: Interventional study. We enrolled patients with clinical diagnoses of bilateral stage V retinopathy of prematurity, confirmed by b-scan ultrasound and programmed for vitrectomy. During surgery we took samples from blood, aqueous, vitreous, and subretinal fluids. The vascular endothelial growth factor concentration in each sample was measured by ELISA reaction. A control sample of aqueous, vitreous and blood was taken from patients with congenital cataract programmed for phacoemulsification. For statistical analysis, a Mann&ndash;Whitney and a Wilcoxon W test was done with a significant P value of 0.05.Results: We took samples of 16 consecutive patients who met the inclusion criteria. The vascular endothelial growth factor levels in the study group were: aqueous, 76.81 &plusmn; 61.89 pg/mL; vitreous, 118.53 &plusmn; 65.87 pg/mL; subretinal fluid, 1636.58 &plusmn; 356.47 pg/mL; and plasma, 74.64 &plusmn; 43.94 pg/mL. There was a statistical difference between the study and the control group (P &lt; 0.001) in the aqueous and vitreous samples.Conclusion: Stage 5 retinopathy of prematurity has elevated intraocular levels of vascular endothelial growth factor, which remains high despite severe retinal lesion. There was no &shy;statistical difference in plasma levels of the molecule between the control and study group.Keywords: VEGF, late stages, ROP Raul Velez-MontoyaCarmen ClappJose Carlos Riveraet alDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2010, Iss default, Pp 947-953 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Raul Velez-Montoya Carmen Clapp Jose Carlos Rivera et al Intraocular and systemic levels of vascular endothelial growth factor in advanced cases of retinopathy of prematurity
description	Raul Velez-Montoya1, Carmen Clapp2, Jose Carlos Rivera2, Gerardo Garcia-Aguirre1, Virgilio Morales-Cant&oacute;n1, Jans Fromow-Guerra1, Jose Luis Guerrero-Naranjo1, Hugo Quiroz-Mercado31Retina Department Asociaci&oacute;n para Evitar la Ceguera en M&eacute;xico IAP, M&eacute;xico City, M&eacute;xico; 2Department of Cellular and Molecular Neurobiology, Universidad Nacional Aut&oacute;noma de M&eacute;xico, Quer&eacute;taro, M&eacute;xico; 3Department of Ophthalmology, Denver Health Medical Center, University of Colorado School of Medicine, Colorado, USAPurpose: To measure vitreous, aqueous, subretinal fluid and plasma levels of vascular &shy;endothelial growth factor in late stages of retinopathy of prematurity.Methods: Interventional study. We enrolled patients with clinical diagnoses of bilateral stage V retinopathy of prematurity, confirmed by b-scan ultrasound and programmed for vitrectomy. During surgery we took samples from blood, aqueous, vitreous, and subretinal fluids. The vascular endothelial growth factor concentration in each sample was measured by ELISA reaction. A control sample of aqueous, vitreous and blood was taken from patients with congenital cataract programmed for phacoemulsification. For statistical analysis, a Mann&ndash;Whitney and a Wilcoxon W test was done with a significant P value of 0.05.Results: We took samples of 16 consecutive patients who met the inclusion criteria. The vascular endothelial growth factor levels in the study group were: aqueous, 76.81 &plusmn; 61.89 pg/mL; vitreous, 118.53 &plusmn; 65.87 pg/mL; subretinal fluid, 1636.58 &plusmn; 356.47 pg/mL; and plasma, 74.64 &plusmn; 43.94 pg/mL. There was a statistical difference between the study and the control group (P &lt; 0.001) in the aqueous and vitreous samples.Conclusion: Stage 5 retinopathy of prematurity has elevated intraocular levels of vascular endothelial growth factor, which remains high despite severe retinal lesion. There was no &shy;statistical difference in plasma levels of the molecule between the control and study group.Keywords: VEGF, late stages, ROP
format	article
author	Raul Velez-Montoya Carmen Clapp Jose Carlos Rivera et al
author_facet	Raul Velez-Montoya Carmen Clapp Jose Carlos Rivera et al
author_sort	Raul Velez-Montoya
title	Intraocular and systemic levels of vascular endothelial growth factor in advanced cases of retinopathy of prematurity
title_short	Intraocular and systemic levels of vascular endothelial growth factor in advanced cases of retinopathy of prematurity
title_full	Intraocular and systemic levels of vascular endothelial growth factor in advanced cases of retinopathy of prematurity
title_fullStr	Intraocular and systemic levels of vascular endothelial growth factor in advanced cases of retinopathy of prematurity
title_full_unstemmed	Intraocular and systemic levels of vascular endothelial growth factor in advanced cases of retinopathy of prematurity
title_sort	intraocular and systemic levels of vascular endothelial growth factor in advanced cases of retinopathy of prematurity
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/1164358f389747d0bea7c10b6a09ce59
work_keys_str_mv	AT raulvelezmontoya intraocularandsystemiclevelsofvascularendothelialgrowthfactorinadvancedcasesofretinopathyofprematurity AT carmenclapp intraocularandsystemiclevelsofvascularendothelialgrowthfactorinadvancedcasesofretinopathyofprematurity AT josecarlosrivera intraocularandsystemiclevelsofvascularendothelialgrowthfactorinadvancedcasesofretinopathyofprematurity AT etal intraocularandsystemiclevelsofvascularendothelialgrowthfactorinadvancedcasesofretinopathyofprematurity
_version_	1718403360988594176

Intraocular and systemic levels of vascular endothelial growth factor in advanced cases of retinopathy of prematurity

Ejemplares similares