Viral Entry Properties Required for Fitness in Humans Are Lost through Rapid Genomic Change during Viral Isolation

IMPORTANCE Human parainfluenza virus 3 is an important cause of morbidity and mortality among infants, the immunocompromised, and the elderly. Using deep genomic sequencing of HPIV-3-positive clinical material and its subsequent viral isolate, we discover a number of known and novel coding mutations...

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Autores principales:	Sho Iketani, Ryan C. Shean, Marion Ferren, Negar Makhsous, Dolly B. Aquino, Amedee des Georges, Bert Rima, Cyrille Mathieu, Matteo Porotto, Anne Moscona, Alexander L. Greninger
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	entry mechanisms lung infection metagenomics parainfluenza virus paramyxovirus viral evolution Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/116787510a404c218ec29e3d5ebc31b9
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spelling	oai:doaj.org-article:116787510a404c218ec29e3d5ebc31b92021-11-15T16:00:15ZViral Entry Properties Required for Fitness in Humans Are Lost through Rapid Genomic Change during Viral Isolation10.1128/mBio.00898-182150-7511https://doaj.org/article/116787510a404c218ec29e3d5ebc31b92018-09-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00898-18https://doaj.org/toc/2150-7511IMPORTANCE Human parainfluenza virus 3 is an important cause of morbidity and mortality among infants, the immunocompromised, and the elderly. Using deep genomic sequencing of HPIV-3-positive clinical material and its subsequent viral isolate, we discover a number of known and novel coding mutations in the main HPIV-3 attachment protein HN during brief exposure to immortalized cells. These mutations significantly alter function of the fusion complex, increasing fusion promotion by HN as well as generally decreasing neuraminidase activity and increasing HN-receptor engagement. These results show that viruses may evolve rapidly in culture even during primary isolation of the virus and before the first passage and reveal features of fitness for humans that are obscured by rapid adaptation to laboratory conditions.Sho IketaniRyan C. SheanMarion FerrenNegar MakhsousDolly B. AquinoAmedee des GeorgesBert RimaCyrille MathieuMatteo PorottoAnne MosconaAlexander L. GreningerAmerican Society for Microbiologyarticleentry mechanismslung infectionmetagenomicsparainfluenza virusparamyxovirusviral evolutionMicrobiologyQR1-502ENmBio, Vol 9, Iss 4 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	entry mechanisms lung infection metagenomics parainfluenza virus paramyxovirus viral evolution Microbiology QR1-502
spellingShingle	entry mechanisms lung infection metagenomics parainfluenza virus paramyxovirus viral evolution Microbiology QR1-502 Sho Iketani Ryan C. Shean Marion Ferren Negar Makhsous Dolly B. Aquino Amedee des Georges Bert Rima Cyrille Mathieu Matteo Porotto Anne Moscona Alexander L. Greninger Viral Entry Properties Required for Fitness in Humans Are Lost through Rapid Genomic Change during Viral Isolation
description	IMPORTANCE Human parainfluenza virus 3 is an important cause of morbidity and mortality among infants, the immunocompromised, and the elderly. Using deep genomic sequencing of HPIV-3-positive clinical material and its subsequent viral isolate, we discover a number of known and novel coding mutations in the main HPIV-3 attachment protein HN during brief exposure to immortalized cells. These mutations significantly alter function of the fusion complex, increasing fusion promotion by HN as well as generally decreasing neuraminidase activity and increasing HN-receptor engagement. These results show that viruses may evolve rapidly in culture even during primary isolation of the virus and before the first passage and reveal features of fitness for humans that are obscured by rapid adaptation to laboratory conditions.
format	article
author	Sho Iketani Ryan C. Shean Marion Ferren Negar Makhsous Dolly B. Aquino Amedee des Georges Bert Rima Cyrille Mathieu Matteo Porotto Anne Moscona Alexander L. Greninger
author_facet	Sho Iketani Ryan C. Shean Marion Ferren Negar Makhsous Dolly B. Aquino Amedee des Georges Bert Rima Cyrille Mathieu Matteo Porotto Anne Moscona Alexander L. Greninger
author_sort	Sho Iketani
title	Viral Entry Properties Required for Fitness in Humans Are Lost through Rapid Genomic Change during Viral Isolation
title_short	Viral Entry Properties Required for Fitness in Humans Are Lost through Rapid Genomic Change during Viral Isolation
title_full	Viral Entry Properties Required for Fitness in Humans Are Lost through Rapid Genomic Change during Viral Isolation
title_fullStr	Viral Entry Properties Required for Fitness in Humans Are Lost through Rapid Genomic Change during Viral Isolation
title_full_unstemmed	Viral Entry Properties Required for Fitness in Humans Are Lost through Rapid Genomic Change during Viral Isolation
title_sort	viral entry properties required for fitness in humans are lost through rapid genomic change during viral isolation
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/116787510a404c218ec29e3d5ebc31b9
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Viral Entry Properties Required for Fitness in Humans Are Lost through Rapid Genomic Change during Viral Isolation

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