DNA barcoding identifies Argentine fishes from marine and brackish waters.

<h4>Background</h4>DNA barcoding has been advanced as a promising tool to aid species identification and discovery through the use of short, standardized gene targets. Despite extensive taxonomic studies, for a variety of reasons the identification of fishes can be problematic, even for...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ezequiel Mabragaña, Juan Martín Díaz de Astarloa, Robert Hanner, Junbin Zhang, Mariano González Castro
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/116d2d8e194d43f8b177a0705e780369
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:116d2d8e194d43f8b177a0705e780369
record_format	dspace
spelling	oai:doaj.org-article:116d2d8e194d43f8b177a0705e7803692021-11-18T07:32:37ZDNA barcoding identifies Argentine fishes from marine and brackish waters.1932-620310.1371/journal.pone.0028655https://doaj.org/article/116d2d8e194d43f8b177a0705e7803692011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22174860/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>DNA barcoding has been advanced as a promising tool to aid species identification and discovery through the use of short, standardized gene targets. Despite extensive taxonomic studies, for a variety of reasons the identification of fishes can be problematic, even for experts. DNA barcoding is proving to be a useful tool in this context. However, its broad application is impeded by the need to construct a comprehensive reference sequence library for all fish species. Here, we make a regional contribution to this grand challenge by calibrating the species discrimination efficiency of barcoding among 125 Argentine fish species, representing nearly one third of the known fauna, and examine the utility of these data to address several key taxonomic uncertainties pertaining to species in this region.<h4>Methodology/principal findings</h4>Specimens were collected and morphologically identified during crusies conducted between 2005 and 2008. The standard BARCODE fragment of COI was amplified and bi-directionally sequenced from 577 specimens (mean of 5 specimens/species), and all specimens and sequence data were archived and interrogated using analytical tools available on the Barcode of Life Data System (BOLD; www.barcodinglife.org). Nearly all species exhibited discrete clusters of closely related haplogroups which permitted the discrimination of 95% of the species (i.e. 119/125) examined while cases of shared haplotypes were detected among just three species-pairs. Notably, barcoding aided the identification of a new species of skate, Dipturus argentinensis, permitted the recognition of Genypterus brasiliensis as a valid species and questions the generic assignment of Paralichthys isosceles.<h4>Conclusions/significance</h4>This study constitutes a significant contribution to the global barcode reference sequence library for fishes and demonstrates the utility of barcoding for regional species identification. As an independent assessment of alpha taxonomy, barcodes provide robust support for most morphologically based taxon concepts and also highlight key areas of taxonomic uncertainty worthy of reappraisal.Ezequiel MabragañaJuan Martín Díaz de AstarloaRobert HannerJunbin ZhangMariano González CastroPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e28655 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ezequiel Mabragaña Juan Martín Díaz de Astarloa Robert Hanner Junbin Zhang Mariano González Castro DNA barcoding identifies Argentine fishes from marine and brackish waters.
description	<h4>Background</h4>DNA barcoding has been advanced as a promising tool to aid species identification and discovery through the use of short, standardized gene targets. Despite extensive taxonomic studies, for a variety of reasons the identification of fishes can be problematic, even for experts. DNA barcoding is proving to be a useful tool in this context. However, its broad application is impeded by the need to construct a comprehensive reference sequence library for all fish species. Here, we make a regional contribution to this grand challenge by calibrating the species discrimination efficiency of barcoding among 125 Argentine fish species, representing nearly one third of the known fauna, and examine the utility of these data to address several key taxonomic uncertainties pertaining to species in this region.<h4>Methodology/principal findings</h4>Specimens were collected and morphologically identified during crusies conducted between 2005 and 2008. The standard BARCODE fragment of COI was amplified and bi-directionally sequenced from 577 specimens (mean of 5 specimens/species), and all specimens and sequence data were archived and interrogated using analytical tools available on the Barcode of Life Data System (BOLD; www.barcodinglife.org). Nearly all species exhibited discrete clusters of closely related haplogroups which permitted the discrimination of 95% of the species (i.e. 119/125) examined while cases of shared haplotypes were detected among just three species-pairs. Notably, barcoding aided the identification of a new species of skate, Dipturus argentinensis, permitted the recognition of Genypterus brasiliensis as a valid species and questions the generic assignment of Paralichthys isosceles.<h4>Conclusions/significance</h4>This study constitutes a significant contribution to the global barcode reference sequence library for fishes and demonstrates the utility of barcoding for regional species identification. As an independent assessment of alpha taxonomy, barcodes provide robust support for most morphologically based taxon concepts and also highlight key areas of taxonomic uncertainty worthy of reappraisal.
format	article
author	Ezequiel Mabragaña Juan Martín Díaz de Astarloa Robert Hanner Junbin Zhang Mariano González Castro
author_facet	Ezequiel Mabragaña Juan Martín Díaz de Astarloa Robert Hanner Junbin Zhang Mariano González Castro
author_sort	Ezequiel Mabragaña
title	DNA barcoding identifies Argentine fishes from marine and brackish waters.
title_short	DNA barcoding identifies Argentine fishes from marine and brackish waters.
title_full	DNA barcoding identifies Argentine fishes from marine and brackish waters.
title_fullStr	DNA barcoding identifies Argentine fishes from marine and brackish waters.
title_full_unstemmed	DNA barcoding identifies Argentine fishes from marine and brackish waters.
title_sort	dna barcoding identifies argentine fishes from marine and brackish waters.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/116d2d8e194d43f8b177a0705e780369
work_keys_str_mv	AT ezequielmabragana dnabarcodingidentifiesargentinefishesfrommarineandbrackishwaters AT juanmartindiazdeastarloa dnabarcodingidentifiesargentinefishesfrommarineandbrackishwaters AT roberthanner dnabarcodingidentifiesargentinefishesfrommarineandbrackishwaters AT junbinzhang dnabarcodingidentifiesargentinefishesfrommarineandbrackishwaters AT marianogonzalezcastro dnabarcodingidentifiesargentinefishesfrommarineandbrackishwaters
_version_	1718423269255675904

DNA barcoding identifies Argentine fishes from marine and brackish waters.

Ejemplares similares