Prospectively isolated mesenchymal stem/stromal cells are enriched in the CD73+ population and exhibit efficacy after transplantation

Abstract Mesenchymal stem/stromal cells (MSCs), which reside in the bone marrow (BM) and various other tissues, can self-renew and differentiate into mesenchymal lineages. Many groups have harvested rat MSCs (rMSCs) from rat BM (rBM) by using a flush-out procedure and have evaluated surface marker e...

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Autores principales:	Eriko Grace Suto, Yo Mabuchi, Nobuharu Suzuki, Koji Suzuki, Yusuke Ogata, Miyu Taguchi, Takeshi Muneta, Ichiro Sekiya, Chihiro Akazawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/11741841962d443481d02aee53a8bfa2
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spelling	oai:doaj.org-article:11741841962d443481d02aee53a8bfa22021-12-02T11:40:53ZProspectively isolated mesenchymal stem/stromal cells are enriched in the CD73+ population and exhibit efficacy after transplantation10.1038/s41598-017-05099-12045-2322https://doaj.org/article/11741841962d443481d02aee53a8bfa22017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05099-1https://doaj.org/toc/2045-2322Abstract Mesenchymal stem/stromal cells (MSCs), which reside in the bone marrow (BM) and various other tissues, can self-renew and differentiate into mesenchymal lineages. Many groups have harvested rat MSCs (rMSCs) from rat BM (rBM) by using a flush-out procedure and have evaluated surface marker expression after long-term culture. However, MSCs gradually differentiate during expansion and exhibit altered proliferation rates, morphological features and functions in vitro. Variations in MSC isolation methods may alter the effectiveness of therapeutic applications. Here, on the basis of CD29 (Itgb1) and CD54 (Icam1) expression, we prospectively isolated a population with a high colony-forming ability and multi-lineage potential from the rBM, and we demonstrated that most of these cells expressed CD73. Successful engraftment of rMSCs was achieved by using a fluorescence-conjugated anti-CD73 antibody. In humans and mice, MSCs were also purified by CD73, thus suggesting that CD73 may serve as a universal marker for prospective isolation of MSCs. Our results may facilitate investigations of MSC properties and function.Eriko Grace SutoYo MabuchiNobuharu SuzukiKoji SuzukiYusuke OgataMiyu TaguchiTakeshi MunetaIchiro SekiyaChihiro AkazawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eriko Grace Suto Yo Mabuchi Nobuharu Suzuki Koji Suzuki Yusuke Ogata Miyu Taguchi Takeshi Muneta Ichiro Sekiya Chihiro Akazawa Prospectively isolated mesenchymal stem/stromal cells are enriched in the CD73+ population and exhibit efficacy after transplantation
description	Abstract Mesenchymal stem/stromal cells (MSCs), which reside in the bone marrow (BM) and various other tissues, can self-renew and differentiate into mesenchymal lineages. Many groups have harvested rat MSCs (rMSCs) from rat BM (rBM) by using a flush-out procedure and have evaluated surface marker expression after long-term culture. However, MSCs gradually differentiate during expansion and exhibit altered proliferation rates, morphological features and functions in vitro. Variations in MSC isolation methods may alter the effectiveness of therapeutic applications. Here, on the basis of CD29 (Itgb1) and CD54 (Icam1) expression, we prospectively isolated a population with a high colony-forming ability and multi-lineage potential from the rBM, and we demonstrated that most of these cells expressed CD73. Successful engraftment of rMSCs was achieved by using a fluorescence-conjugated anti-CD73 antibody. In humans and mice, MSCs were also purified by CD73, thus suggesting that CD73 may serve as a universal marker for prospective isolation of MSCs. Our results may facilitate investigations of MSC properties and function.
format	article
author	Eriko Grace Suto Yo Mabuchi Nobuharu Suzuki Koji Suzuki Yusuke Ogata Miyu Taguchi Takeshi Muneta Ichiro Sekiya Chihiro Akazawa
author_facet	Eriko Grace Suto Yo Mabuchi Nobuharu Suzuki Koji Suzuki Yusuke Ogata Miyu Taguchi Takeshi Muneta Ichiro Sekiya Chihiro Akazawa
author_sort	Eriko Grace Suto
title	Prospectively isolated mesenchymal stem/stromal cells are enriched in the CD73+ population and exhibit efficacy after transplantation
title_short	Prospectively isolated mesenchymal stem/stromal cells are enriched in the CD73+ population and exhibit efficacy after transplantation
title_full	Prospectively isolated mesenchymal stem/stromal cells are enriched in the CD73+ population and exhibit efficacy after transplantation
title_fullStr	Prospectively isolated mesenchymal stem/stromal cells are enriched in the CD73+ population and exhibit efficacy after transplantation
title_full_unstemmed	Prospectively isolated mesenchymal stem/stromal cells are enriched in the CD73+ population and exhibit efficacy after transplantation
title_sort	prospectively isolated mesenchymal stem/stromal cells are enriched in the cd73+ population and exhibit efficacy after transplantation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/11741841962d443481d02aee53a8bfa2
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Prospectively isolated mesenchymal stem/stromal cells are enriched in the CD73+ population and exhibit efficacy after transplantation

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