Green Synthesis of Silver Nanoparticles Using the Extract of Lonicera Nummulariifolia and Investigating Its Antioxidant, Antimicrobial and Anticancer Effects Against Lung Cancer Cell Line A549

BACKGROUND AND OBJECTIVE: Due to the increasing incidence of cancer-related deaths and the deficiencies of chemotherapy and radiotherapy in advanced forms of cancer, new approaches are needed to control cancer, and one of these techniques is the use of nanoparticles, especially silver nanoparticles....

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Autores principales:	H Rezaie, SMM HAmdi, A Mirzaie
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2019
Materias:	lonicera nummulariifolia silver nanoparticles antioxidant effects antimicrobial effects anticancer effects lung cancer. Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/117ae0f986524cd1baa801bc0e718bb7
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spelling	oai:doaj.org-article:117ae0f986524cd1baa801bc0e718bb72021-11-10T08:30:36ZGreen Synthesis of Silver Nanoparticles Using the Extract of Lonicera Nummulariifolia and Investigating Its Antioxidant, Antimicrobial and Anticancer Effects Against Lung Cancer Cell Line A5491561-41072251-7170https://doaj.org/article/117ae0f986524cd1baa801bc0e718bb72019-12-01T00:00:00Zhttp://jbums.org/article-1-8250-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Due to the increasing incidence of cancer-related deaths and the deficiencies of chemotherapy and radiotherapy in advanced forms of cancer, new approaches are needed to control cancer, and one of these techniques is the use of nanoparticles, especially silver nanoparticles. Nowadays, the use of plants for the synthesis of silver nanoparticles has attracted the attention of researchers due to their low cost. Therefore, the aim of this study was to investigate the green synthesis of silver nanoparticles using the extract of lonicera nummulariifolia and to study its antioxidant, antimicrobial and anticancer effects against lung cancer cell line A549. METHODS: In this experimental study, silver nanoparticles were synthesized using the extract of lonicera nummulariifolia as a regenerative agent. The antioxidant effects of synthesized silver nanoparticles were evaluated by DPPH assay and finally its antimicrobial and anticancer activity were respectively evaluated by Broth Microdilution and MTT assays at concentrations of 3.125, 6.25, 12.5, 25, 50, 100 μg/ml on lung cancer cell line within 24 h. FINDINGS: The results of DPPH assay showed that the synthesized silver nanoparticles at 100 µg/ml had an antioxidant effect of 33.77±0.83. Antimicrobial test results showed that the antimicrobial effects of silver nanoparticles were greater on gram negative bacteria. MTT results also showed that cell viability was 70.33±0.21 (p>0.05), 51.66±0.24 (p<0.05) 35.75±0.35 (p<0.01), 20.66±0.28 (p<0.001), 13.5±0.31 (p<0.001), and 7.6±0.37 (p<0.001), respectively. Results of DPPH assay showed that silver nanoparticles has significant antioxidant effects (p<0.05). CONCLUSION: Considering the antimicrobial and anticancer effects of synthesized silver nanoparticles, it can be used as a drug candidate.H RezaieSMM HAmdiA MirzaieBabol University of Medical Sciencesarticlelonicera nummulariifoliasilver nanoparticlesantioxidant effectsantimicrobial effectsanticancer effectslung cancer.MedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 21, Iss 1, Pp 207-214 (2019)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	lonicera nummulariifolia silver nanoparticles antioxidant effects antimicrobial effects anticancer effects lung cancer. Medicine R Medicine (General) R5-920
spellingShingle	lonicera nummulariifolia silver nanoparticles antioxidant effects antimicrobial effects anticancer effects lung cancer. Medicine R Medicine (General) R5-920 H Rezaie SMM HAmdi A Mirzaie Green Synthesis of Silver Nanoparticles Using the Extract of Lonicera Nummulariifolia and Investigating Its Antioxidant, Antimicrobial and Anticancer Effects Against Lung Cancer Cell Line A549
description	BACKGROUND AND OBJECTIVE: Due to the increasing incidence of cancer-related deaths and the deficiencies of chemotherapy and radiotherapy in advanced forms of cancer, new approaches are needed to control cancer, and one of these techniques is the use of nanoparticles, especially silver nanoparticles. Nowadays, the use of plants for the synthesis of silver nanoparticles has attracted the attention of researchers due to their low cost. Therefore, the aim of this study was to investigate the green synthesis of silver nanoparticles using the extract of lonicera nummulariifolia and to study its antioxidant, antimicrobial and anticancer effects against lung cancer cell line A549. METHODS: In this experimental study, silver nanoparticles were synthesized using the extract of lonicera nummulariifolia as a regenerative agent. The antioxidant effects of synthesized silver nanoparticles were evaluated by DPPH assay and finally its antimicrobial and anticancer activity were respectively evaluated by Broth Microdilution and MTT assays at concentrations of 3.125, 6.25, 12.5, 25, 50, 100 μg/ml on lung cancer cell line within 24 h. FINDINGS: The results of DPPH assay showed that the synthesized silver nanoparticles at 100 µg/ml had an antioxidant effect of 33.77±0.83. Antimicrobial test results showed that the antimicrobial effects of silver nanoparticles were greater on gram negative bacteria. MTT results also showed that cell viability was 70.33±0.21 (p>0.05), 51.66±0.24 (p<0.05) 35.75±0.35 (p<0.01), 20.66±0.28 (p<0.001), 13.5±0.31 (p<0.001), and 7.6±0.37 (p<0.001), respectively. Results of DPPH assay showed that silver nanoparticles has significant antioxidant effects (p<0.05). CONCLUSION: Considering the antimicrobial and anticancer effects of synthesized silver nanoparticles, it can be used as a drug candidate.
format	article
author	H Rezaie SMM HAmdi A Mirzaie
author_facet	H Rezaie SMM HAmdi A Mirzaie
author_sort	H Rezaie
title	Green Synthesis of Silver Nanoparticles Using the Extract of Lonicera Nummulariifolia and Investigating Its Antioxidant, Antimicrobial and Anticancer Effects Against Lung Cancer Cell Line A549
title_short	Green Synthesis of Silver Nanoparticles Using the Extract of Lonicera Nummulariifolia and Investigating Its Antioxidant, Antimicrobial and Anticancer Effects Against Lung Cancer Cell Line A549
title_full	Green Synthesis of Silver Nanoparticles Using the Extract of Lonicera Nummulariifolia and Investigating Its Antioxidant, Antimicrobial and Anticancer Effects Against Lung Cancer Cell Line A549
title_fullStr	Green Synthesis of Silver Nanoparticles Using the Extract of Lonicera Nummulariifolia and Investigating Its Antioxidant, Antimicrobial and Anticancer Effects Against Lung Cancer Cell Line A549
title_full_unstemmed	Green Synthesis of Silver Nanoparticles Using the Extract of Lonicera Nummulariifolia and Investigating Its Antioxidant, Antimicrobial and Anticancer Effects Against Lung Cancer Cell Line A549
title_sort	green synthesis of silver nanoparticles using the extract of lonicera nummulariifolia and investigating its antioxidant, antimicrobial and anticancer effects against lung cancer cell line a549
publisher	Babol University of Medical Sciences
publishDate	2019
url	https://doaj.org/article/117ae0f986524cd1baa801bc0e718bb7
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_version_	1718440349537402880

Green Synthesis of Silver Nanoparticles Using the Extract of Lonicera Nummulariifolia and Investigating Its Antioxidant, Antimicrobial and Anticancer Effects Against Lung Cancer Cell Line A549

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