The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40.

<h4>Background</h4>Hepatic stellate cells (HSCs) are one of the main cell types involved in liver fibrosis induced by many factors, including schistosomes. Previous studies in our lab have shown that recombinant P40 protein from Schistosoma japonicum (rSjP40) can inhibit HSC activation i...

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Autores principales:	Xiaolei Sun, Li Zhang, Yuting Jiang, Aihong Li, Dandan Zhu, Jiangrong Wu, Yinong Duan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/117b2bf55c2043a7b8982840eae57e84
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spelling	oai:doaj.org-article:117b2bf55c2043a7b8982840eae57e842021-11-25T06:33:07ZThe role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40.1935-27271935-273510.1371/journal.pntd.0009472https://doaj.org/article/117b2bf55c2043a7b8982840eae57e842021-06-01T00:00:00Zhttps://doi.org/10.1371/journal.pntd.0009472https://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>Hepatic stellate cells (HSCs) are one of the main cell types involved in liver fibrosis induced by many factors, including schistosomes. Previous studies in our lab have shown that recombinant P40 protein from Schistosoma japonicum (rSjP40) can inhibit HSC activation in vitro. Let-7b is a member of the let-7 microRNA family and plays an inhibitory role in a variety of diseases and inflammatory conditions. In this study, we investigated the role of let-7b in the inhibition of HSC activation by rSjP40.<h4>Methods</h4>Expression of let-7b was detected by quantitative real-time PCR. A dual luciferase assay was used to confirm direct interaction between let-7b and collagen I. We also used western blot to assess protein levels of TGFβRI and collagen type I α1 (COL1A1).<h4>Results</h4>We found that rSjP40 up-regulates expression of let-7b in HSCs. Let-7b inhibits collagen I expression by directly targeting the 3'UTR region of the collagen I gene. Furthermore, we discovered that let-7b inhibitor partially restores the loss of collagen I expression caused by rSjP40.<h4>Conclusion</h4>Our research clarifies the role of let-7b in the inhibition of HSC activation by rSjP40 and will provide new insights and ideas for the inhibition of HSC activation and treatment of liver fibrosis.Xiaolei SunLi ZhangYuting JiangAihong LiDandan ZhuJiangrong WuYinong DuanPublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009472 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270
spellingShingle	Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Xiaolei Sun Li Zhang Yuting Jiang Aihong Li Dandan Zhu Jiangrong Wu Yinong Duan The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40.
description	<h4>Background</h4>Hepatic stellate cells (HSCs) are one of the main cell types involved in liver fibrosis induced by many factors, including schistosomes. Previous studies in our lab have shown that recombinant P40 protein from Schistosoma japonicum (rSjP40) can inhibit HSC activation in vitro. Let-7b is a member of the let-7 microRNA family and plays an inhibitory role in a variety of diseases and inflammatory conditions. In this study, we investigated the role of let-7b in the inhibition of HSC activation by rSjP40.<h4>Methods</h4>Expression of let-7b was detected by quantitative real-time PCR. A dual luciferase assay was used to confirm direct interaction between let-7b and collagen I. We also used western blot to assess protein levels of TGFβRI and collagen type I α1 (COL1A1).<h4>Results</h4>We found that rSjP40 up-regulates expression of let-7b in HSCs. Let-7b inhibits collagen I expression by directly targeting the 3'UTR region of the collagen I gene. Furthermore, we discovered that let-7b inhibitor partially restores the loss of collagen I expression caused by rSjP40.<h4>Conclusion</h4>Our research clarifies the role of let-7b in the inhibition of HSC activation by rSjP40 and will provide new insights and ideas for the inhibition of HSC activation and treatment of liver fibrosis.
format	article
author	Xiaolei Sun Li Zhang Yuting Jiang Aihong Li Dandan Zhu Jiangrong Wu Yinong Duan
author_facet	Xiaolei Sun Li Zhang Yuting Jiang Aihong Li Dandan Zhu Jiangrong Wu Yinong Duan
author_sort	Xiaolei Sun
title	The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40.
title_short	The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40.
title_full	The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40.
title_fullStr	The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40.
title_full_unstemmed	The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40.
title_sort	role of let-7b in the inhibition of hepatic stellate cell activation by rsjp40.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/117b2bf55c2043a7b8982840eae57e84
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The role of let-7b in the inhibition of hepatic stellate cell activation by rSjP40.

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