Photofungizides Based on Curcumin and Derivates Thereof against <i>Candida albicans</i> and <i>Aspergillus niger</i>

Fungal infections in humans, contamination of food and structural damage to buildings by fungi are associated with high costs for the general public. In addition, the increase in antifungal resistance towards conventional treatment raises the demand for new fungicidal methods. Here, we present the a...

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Autores principales:	Barbara Schamberger, Kristjan Plaetzer
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	photodynamic inactivation photosensitizer antifungal curcumin <i>Candida albicans</i> <i>Aspergillus niger</i> Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/117cd405efd043c9bc4f1883015d356d
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spelling	oai:doaj.org-article:117cd405efd043c9bc4f1883015d356d2021-11-25T16:22:39ZPhotofungizides Based on Curcumin and Derivates Thereof against <i>Candida albicans</i> and <i>Aspergillus niger</i>10.3390/antibiotics101113152079-6382https://doaj.org/article/117cd405efd043c9bc4f1883015d356d2021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1315https://doaj.org/toc/2079-6382Fungal infections in humans, contamination of food and structural damage to buildings by fungi are associated with high costs for the general public. In addition, the increase in antifungal resistance towards conventional treatment raises the demand for new fungicidal methods. Here, we present the antifungal use of Photodynamic Inactivation (PDI) based on the natural photosensitizer curcumin and a water-soluble positively charged derivative thereof (SA-CUR 12a) against two different model organisms; <i>Candida albicans</i> grown in a liquid culture and photo treated with a 435 nm LED light followed by counting of the colony-forming units and photoinactivation of tissue-like hyphal spheres of <i>Aspergillus niger</i> (diameter ~5 mm) with subsequent monitoring of colony growth. Curcumin (50 µM, no incubation period, i.p.) supplemented with 10% or 0.5% DMSO as well as SA-CUR 12a (50 µM no i.p or 5 min i.p.) triggered a photoantifungal effect of >4 log units towards <i>C. albicans</i>. At 100 µM, SA-CUR 12a (0 min or 5 min i.p.) achieved a reduction of >6 log units. Colonies of <i>A. niger</i> shrunk significantly during PDI treatment. Photoinactivation with 50 µM or 100 µM curcumin (+0.5% DMSO) resulted in complete growth inhibition. PDI using 20, 50 or 100 µM SA-CUR 12a (with or without 10% DMSO) also showed a significant reduction in colony area compared to the control after 48 h, although less pronounced compared to curcumin. In summary, PDI using curcumin or SA-CUR 12a against <i>C. albicans</i> or <i>A. niger</i> is a promising alternative to currently used fungicides, with the advantage of being very unlikely to induce resistance.Barbara SchambergerKristjan PlaetzerMDPI AGarticlephotodynamic inactivationphotosensitizerantifungalcurcumin<i>Candida albicans</i><i>Aspergillus niger</i>Therapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1315, p 1315 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	photodynamic inactivation photosensitizer antifungal curcumin <i>Candida albicans</i> <i>Aspergillus niger</i> Therapeutics. Pharmacology RM1-950
spellingShingle	photodynamic inactivation photosensitizer antifungal curcumin <i>Candida albicans</i> <i>Aspergillus niger</i> Therapeutics. Pharmacology RM1-950 Barbara Schamberger Kristjan Plaetzer Photofungizides Based on Curcumin and Derivates Thereof against <i>Candida albicans</i> and <i>Aspergillus niger</i>
description	Fungal infections in humans, contamination of food and structural damage to buildings by fungi are associated with high costs for the general public. In addition, the increase in antifungal resistance towards conventional treatment raises the demand for new fungicidal methods. Here, we present the antifungal use of Photodynamic Inactivation (PDI) based on the natural photosensitizer curcumin and a water-soluble positively charged derivative thereof (SA-CUR 12a) against two different model organisms; <i>Candida albicans</i> grown in a liquid culture and photo treated with a 435 nm LED light followed by counting of the colony-forming units and photoinactivation of tissue-like hyphal spheres of <i>Aspergillus niger</i> (diameter ~5 mm) with subsequent monitoring of colony growth. Curcumin (50 µM, no incubation period, i.p.) supplemented with 10% or 0.5% DMSO as well as SA-CUR 12a (50 µM no i.p or 5 min i.p.) triggered a photoantifungal effect of >4 log units towards <i>C. albicans</i>. At 100 µM, SA-CUR 12a (0 min or 5 min i.p.) achieved a reduction of >6 log units. Colonies of <i>A. niger</i> shrunk significantly during PDI treatment. Photoinactivation with 50 µM or 100 µM curcumin (+0.5% DMSO) resulted in complete growth inhibition. PDI using 20, 50 or 100 µM SA-CUR 12a (with or without 10% DMSO) also showed a significant reduction in colony area compared to the control after 48 h, although less pronounced compared to curcumin. In summary, PDI using curcumin or SA-CUR 12a against <i>C. albicans</i> or <i>A. niger</i> is a promising alternative to currently used fungicides, with the advantage of being very unlikely to induce resistance.
format	article
author	Barbara Schamberger Kristjan Plaetzer
author_facet	Barbara Schamberger Kristjan Plaetzer
author_sort	Barbara Schamberger
title	Photofungizides Based on Curcumin and Derivates Thereof against <i>Candida albicans</i> and <i>Aspergillus niger</i>
title_short	Photofungizides Based on Curcumin and Derivates Thereof against <i>Candida albicans</i> and <i>Aspergillus niger</i>
title_full	Photofungizides Based on Curcumin and Derivates Thereof against <i>Candida albicans</i> and <i>Aspergillus niger</i>
title_fullStr	Photofungizides Based on Curcumin and Derivates Thereof against <i>Candida albicans</i> and <i>Aspergillus niger</i>
title_full_unstemmed	Photofungizides Based on Curcumin and Derivates Thereof against <i>Candida albicans</i> and <i>Aspergillus niger</i>
title_sort	photofungizides based on curcumin and derivates thereof against <i>candida albicans</i> and <i>aspergillus niger</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/117cd405efd043c9bc4f1883015d356d
work_keys_str_mv	AT barbaraschamberger photofungizidesbasedoncurcuminandderivatesthereofagainsticandidaalbicansiandiaspergillusnigeri AT kristjanplaetzer photofungizidesbasedoncurcuminandderivatesthereofagainsticandidaalbicansiandiaspergillusnigeri
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Photofungizides Based on Curcumin and Derivates Thereof against <i>Candida albicans</i> and <i>Aspergillus niger</i>

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