Impact of surface adhesion and sample heterogeneity on the multiscale mechanical characterisation of soft biomaterials

Abstract The mechanical properties of soft materials used in the biomedical field play an important role on their performance. In the field of tissue engineering, it is known that cells sense the mechanical properties of their environment, however some materials, such as Sylard 184 PDMS (poly(dimeth...

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Autores principales:	W. Megone, N. Roohpour, J. E. Gautrot
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1187bbb8a8924173b19b57954dc59fc8
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spelling	oai:doaj.org-article:1187bbb8a8924173b19b57954dc59fc82021-12-02T16:07:51ZImpact of surface adhesion and sample heterogeneity on the multiscale mechanical characterisation of soft biomaterials10.1038/s41598-018-24671-x2045-2322https://doaj.org/article/1187bbb8a8924173b19b57954dc59fc82018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-24671-xhttps://doaj.org/toc/2045-2322Abstract The mechanical properties of soft materials used in the biomedical field play an important role on their performance. In the field of tissue engineering, it is known that cells sense the mechanical properties of their environment, however some materials, such as Sylard 184 PDMS (poly(dimethylsiloxane)), have failed to elicit such response. It was proposed that differences in the mechanical properties of such soft materials, at different scales, could account for these discrepancies. Indeed, the variation in the elastic moduli obtained for soft materials characterised at different scales can span several orders of magnitude. This called for a side-by-side comparison of the mechanical behaviour of soft materials at different scales. Here we use indentation, rheology and atomic force microscopy nanoidentation (using different tip geometries) to characterise the mechanical properties of PDMS, poly(acrylamide) (PAAm) and carboxymethyl cellulose (CMC) hydrogels at different length scales. Our results highlight the importance of surface adhesion and the resulting changes in contact area, and sample microstructural heterogeneity, in particular for the mechanical characterisation of ultra-soft substrates at the nano- to micro-scale.W. MegoneN. RoohpourJ. E. GautrotNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q W. Megone N. Roohpour J. E. Gautrot Impact of surface adhesion and sample heterogeneity on the multiscale mechanical characterisation of soft biomaterials
description	Abstract The mechanical properties of soft materials used in the biomedical field play an important role on their performance. In the field of tissue engineering, it is known that cells sense the mechanical properties of their environment, however some materials, such as Sylard 184 PDMS (poly(dimethylsiloxane)), have failed to elicit such response. It was proposed that differences in the mechanical properties of such soft materials, at different scales, could account for these discrepancies. Indeed, the variation in the elastic moduli obtained for soft materials characterised at different scales can span several orders of magnitude. This called for a side-by-side comparison of the mechanical behaviour of soft materials at different scales. Here we use indentation, rheology and atomic force microscopy nanoidentation (using different tip geometries) to characterise the mechanical properties of PDMS, poly(acrylamide) (PAAm) and carboxymethyl cellulose (CMC) hydrogels at different length scales. Our results highlight the importance of surface adhesion and the resulting changes in contact area, and sample microstructural heterogeneity, in particular for the mechanical characterisation of ultra-soft substrates at the nano- to micro-scale.
format	article
author	W. Megone N. Roohpour J. E. Gautrot
author_facet	W. Megone N. Roohpour J. E. Gautrot
author_sort	W. Megone
title	Impact of surface adhesion and sample heterogeneity on the multiscale mechanical characterisation of soft biomaterials
title_short	Impact of surface adhesion and sample heterogeneity on the multiscale mechanical characterisation of soft biomaterials
title_full	Impact of surface adhesion and sample heterogeneity on the multiscale mechanical characterisation of soft biomaterials
title_fullStr	Impact of surface adhesion and sample heterogeneity on the multiscale mechanical characterisation of soft biomaterials
title_full_unstemmed	Impact of surface adhesion and sample heterogeneity on the multiscale mechanical characterisation of soft biomaterials
title_sort	impact of surface adhesion and sample heterogeneity on the multiscale mechanical characterisation of soft biomaterials
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/1187bbb8a8924173b19b57954dc59fc8
work_keys_str_mv	AT wmegone impactofsurfaceadhesionandsampleheterogeneityonthemultiscalemechanicalcharacterisationofsoftbiomaterials AT nroohpour impactofsurfaceadhesionandsampleheterogeneityonthemultiscalemechanicalcharacterisationofsoftbiomaterials AT jegautrot impactofsurfaceadhesionandsampleheterogeneityonthemultiscalemechanicalcharacterisationofsoftbiomaterials
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Impact of surface adhesion and sample heterogeneity on the multiscale mechanical characterisation of soft biomaterials

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