Potential of Lactic Acid Bacteria From Tape and Jember Tempeh as a Probiotic Candidate

Probiotics are microbes in fermented foods that have beneficial effects on health. Microbes that act as probiotics are lactic acid bacteria (LAB) that can produce metabolites such as lactic acid, hydrogen peroxide, and bacteriocins. This study aimed to obtain lactic acid bacterial isolates from tape...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Siti Nur Azizah, Mikhania Christiningtyas Eryani, Azizah Azizah
Formato:	article
Lenguaje:	EN ID
Publicado:	UIN Sunan Gunung Djati 2021
Materias:	antidiarrheal, lactic acid bacteria, probiotic, tape, tempeh Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1199d79285a84aac85c91f5adf255e0e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1199d79285a84aac85c91f5adf255e0e
record_format	dspace
spelling	oai:doaj.org-article:1199d79285a84aac85c91f5adf255e0e2021-11-30T11:51:07ZPotential of Lactic Acid Bacteria From Tape and Jember Tempeh as a Probiotic Candidate2541-42082548-160610.15575/biodjati.v6i2.12393https://doaj.org/article/1199d79285a84aac85c91f5adf255e0e2021-11-01T00:00:00Zhttps://journal.uinsgd.ac.id/index.php/biodjati/article/view/12393https://doaj.org/toc/2541-4208https://doaj.org/toc/2548-1606Probiotics are microbes in fermented foods that have beneficial effects on health. Microbes that act as probiotics are lactic acid bacteria (LAB) that can produce metabolites such as lactic acid, hydrogen peroxide, and bacteriocins. This study aimed to obtain lactic acid bacterial isolates from tape and tempeh, and to test the potential of LAB as a probiotic candidate by activity test as an antidiarrhea and its resistance to gastric pH and bile salts. The fermentation products used as a source of LAB isolates are tempeh sumber mas merk, and yellow cassava tape, sari madu merk from Jember. The results of the first stage regarding the isolation of LAB using GYP media showed that there were 2 LAB isolates (TaJ.14 and TaJ.15) from the tape and 4 LAB isolates (TeJ.18, TeJ.22, TeJ.24, and TeJ.25) from tempeh. The results of the antidiarrheal test using the disc diffusion method (oxoid) showed that TaJ.14 and TaJ.15 isolates were able to inhibit Bacillus subtilis, Escherichia coli, and Shigella dysentriae, while TeJ.18, TeJ.22, TeJ.24, TeJ.25, and Lactobacillus casei (control) was only able to inhibit B. subtilis and E. coli. The results of LAB resistance to gastric pH showed that the TeJ.25 isolate had the highest percentage of pH 3 and 2.5 resistance (51.13 and 33.03%) compared to other isolates and controls. LAB resistance test results against bile salts (oxgal) showed that the TeJ.22 isolate had the highest percentage of resistance (75.10%) compared to other isolates although was still higher in control (75.99%).Siti Nur AzizahMikhania Christiningtyas EryaniAzizah AzizahUIN Sunan Gunung Djatiarticleantidiarrheal, lactic acid bacteria, probiotic, tape, tempehBiology (General)QH301-705.5ENIDJurnal Biodjati, Vol 6, Iss 2, Pp 273-283 (2021)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	antidiarrheal, lactic acid bacteria, probiotic, tape, tempeh Biology (General) QH301-705.5
spellingShingle	antidiarrheal, lactic acid bacteria, probiotic, tape, tempeh Biology (General) QH301-705.5 Siti Nur Azizah Mikhania Christiningtyas Eryani Azizah Azizah Potential of Lactic Acid Bacteria From Tape and Jember Tempeh as a Probiotic Candidate
description	Probiotics are microbes in fermented foods that have beneficial effects on health. Microbes that act as probiotics are lactic acid bacteria (LAB) that can produce metabolites such as lactic acid, hydrogen peroxide, and bacteriocins. This study aimed to obtain lactic acid bacterial isolates from tape and tempeh, and to test the potential of LAB as a probiotic candidate by activity test as an antidiarrhea and its resistance to gastric pH and bile salts. The fermentation products used as a source of LAB isolates are tempeh sumber mas merk, and yellow cassava tape, sari madu merk from Jember. The results of the first stage regarding the isolation of LAB using GYP media showed that there were 2 LAB isolates (TaJ.14 and TaJ.15) from the tape and 4 LAB isolates (TeJ.18, TeJ.22, TeJ.24, and TeJ.25) from tempeh. The results of the antidiarrheal test using the disc diffusion method (oxoid) showed that TaJ.14 and TaJ.15 isolates were able to inhibit Bacillus subtilis, Escherichia coli, and Shigella dysentriae, while TeJ.18, TeJ.22, TeJ.24, TeJ.25, and Lactobacillus casei (control) was only able to inhibit B. subtilis and E. coli. The results of LAB resistance to gastric pH showed that the TeJ.25 isolate had the highest percentage of pH 3 and 2.5 resistance (51.13 and 33.03%) compared to other isolates and controls. LAB resistance test results against bile salts (oxgal) showed that the TeJ.22 isolate had the highest percentage of resistance (75.10%) compared to other isolates although was still higher in control (75.99%).
format	article
author	Siti Nur Azizah Mikhania Christiningtyas Eryani Azizah Azizah
author_facet	Siti Nur Azizah Mikhania Christiningtyas Eryani Azizah Azizah
author_sort	Siti Nur Azizah
title	Potential of Lactic Acid Bacteria From Tape and Jember Tempeh as a Probiotic Candidate
title_short	Potential of Lactic Acid Bacteria From Tape and Jember Tempeh as a Probiotic Candidate
title_full	Potential of Lactic Acid Bacteria From Tape and Jember Tempeh as a Probiotic Candidate
title_fullStr	Potential of Lactic Acid Bacteria From Tape and Jember Tempeh as a Probiotic Candidate
title_full_unstemmed	Potential of Lactic Acid Bacteria From Tape and Jember Tempeh as a Probiotic Candidate
title_sort	potential of lactic acid bacteria from tape and jember tempeh as a probiotic candidate
publisher	UIN Sunan Gunung Djati
publishDate	2021
url	https://doaj.org/article/1199d79285a84aac85c91f5adf255e0e
work_keys_str_mv	AT sitinurazizah potentialoflacticacidbacteriafromtapeandjembertempehasaprobioticcandidate AT mikhaniachristiningtyaseryani potentialoflacticacidbacteriafromtapeandjembertempehasaprobioticcandidate AT azizahazizah potentialoflacticacidbacteriafromtapeandjembertempehasaprobioticcandidate
_version_	1718406605911883776

Potential of Lactic Acid Bacteria From Tape and Jember Tempeh as a Probiotic Candidate

Ejemplares similares