Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit

In order to develop microbial additives for rabbit feed, a spore-forming bacteria was isolated from the feces of Hyla rabbit using reinforced clostridium medium (RCM). The 16S rDNA sequence of the bacterium was subjected to pairwise sequence alignment using BLAST; the colony morphology, and physiolo...

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Autores principales:	Ruiguang Gong, Xiangyang Ye, Shuhui Wang, Zhanjun Ren
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/11aa49c0e4a6401b96966990cd13ef48
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spelling	oai:doaj.org-article:11aa49c0e4a6401b96966990cd13ef482021-11-25T05:54:25ZIsolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit1932-6203https://doaj.org/article/11aa49c0e4a6401b96966990cd13ef482021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8592454/?tool=EBIhttps://doaj.org/toc/1932-6203In order to develop microbial additives for rabbit feed, a spore-forming bacteria was isolated from the feces of Hyla rabbit using reinforced clostridium medium (RCM). The 16S rDNA sequence of the bacterium was subjected to pairwise sequence alignment using BLAST; the colony morphology, and physiological, biochemical, and stress resistance were studied. The results showed that the bacterium was Clostridium sartagoforme, a gram positive anaerobe, which can produce spores. The colony diameter was 0.5 mm—2.5 mm, the diameter of the bacteria was 0.5 μm—1.0 μm × 2.0 μm—6.3 μm, and the spore diameter was 1 μm—1.2 μm × 1 μm—1.2 μm. C. sartagoforme can utilize various sugars and alcohols such as fructose, galactose, sorbitol, and inositol. It secreted cellulase into the extracellular environment to form a transparent hydrolysis circle in Congo red medium, it could not liquify gelatin, and the lysine decarboxylase reaction was positive. In liquid medium it entered the stable growth period after 9 h of inoculation. Additionally, it had good stress resistance with a survival rate that exceeded 53% after gastric juice (pH 2.5) treatment for 3 h, it grew in a medium with a bile salt concentration of 0.3%, and the survival rate exceeded 85% after 10 minutes at 80°C. Moreover, animal testing indicated that this strain has no adverse effects on the morbidity and mortality of rabbits. In summary, C. sartagoforme XN-T4 was isolated from rabbit feces. This bacterium has good resistance to stress, can decompose a variety of monosaccharides and polysaccharides including cellulose, which is relatively harmless for animal health.Ruiguang GongXiangyang YeShuhui WangZhanjun RenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ruiguang Gong Xiangyang Ye Shuhui Wang Zhanjun Ren Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit
description	In order to develop microbial additives for rabbit feed, a spore-forming bacteria was isolated from the feces of Hyla rabbit using reinforced clostridium medium (RCM). The 16S rDNA sequence of the bacterium was subjected to pairwise sequence alignment using BLAST; the colony morphology, and physiological, biochemical, and stress resistance were studied. The results showed that the bacterium was Clostridium sartagoforme, a gram positive anaerobe, which can produce spores. The colony diameter was 0.5 mm—2.5 mm, the diameter of the bacteria was 0.5 μm—1.0 μm × 2.0 μm—6.3 μm, and the spore diameter was 1 μm—1.2 μm × 1 μm—1.2 μm. C. sartagoforme can utilize various sugars and alcohols such as fructose, galactose, sorbitol, and inositol. It secreted cellulase into the extracellular environment to form a transparent hydrolysis circle in Congo red medium, it could not liquify gelatin, and the lysine decarboxylase reaction was positive. In liquid medium it entered the stable growth period after 9 h of inoculation. Additionally, it had good stress resistance with a survival rate that exceeded 53% after gastric juice (pH 2.5) treatment for 3 h, it grew in a medium with a bile salt concentration of 0.3%, and the survival rate exceeded 85% after 10 minutes at 80°C. Moreover, animal testing indicated that this strain has no adverse effects on the morbidity and mortality of rabbits. In summary, C. sartagoforme XN-T4 was isolated from rabbit feces. This bacterium has good resistance to stress, can decompose a variety of monosaccharides and polysaccharides including cellulose, which is relatively harmless for animal health.
format	article
author	Ruiguang Gong Xiangyang Ye Shuhui Wang Zhanjun Ren
author_facet	Ruiguang Gong Xiangyang Ye Shuhui Wang Zhanjun Ren
author_sort	Ruiguang Gong
title	Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit
title_short	Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit
title_full	Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit
title_fullStr	Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit
title_full_unstemmed	Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit
title_sort	isolation, identification, and biological characteristics of clostridium sartagoforme from rabbit
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/11aa49c0e4a6401b96966990cd13ef48
work_keys_str_mv	AT ruiguanggong isolationidentificationandbiologicalcharacteristicsofclostridiumsartagoformefromrabbit AT xiangyangye isolationidentificationandbiologicalcharacteristicsofclostridiumsartagoformefromrabbit AT shuhuiwang isolationidentificationandbiologicalcharacteristicsofclostridiumsartagoformefromrabbit AT zhanjunren isolationidentificationandbiologicalcharacteristicsofclostridiumsartagoformefromrabbit
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Isolation, identification, and biological characteristics of Clostridium sartagoforme from rabbit

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