Three-Dimensional Iron Oxide Nanoparticle-Based Contrast-Enhanced Magnetic Resonance Imaging for Characterization of Cerebral Arteriogenesis in the Mouse Neocortex

Purpose: Subsurface blood vessels in the cerebral cortex have been identified as a bottleneck in cerebral perfusion with the potential for collateral remodeling. However, valid techniques for non-invasive, longitudinal characterization of neocortical microvessels are still lacking. In this study, we...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Till de Bortoli, Philipp Boehm-Sturm, Stefan P. Koch, Melina Nieminen-Kelhä, Lars Wessels, Susanne Mueller, Giovanna D. Ielacqua, Jan Klohs, Peter Vajkoczy, Nils Hecht
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	contrast-enhanced MRI cerebral blood volume iron oxide nanoparticles stroke cerebrovascular disease vessel imaging Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/11bcb034aa864a1d82d3645863352de8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:11bcb034aa864a1d82d3645863352de8
record_format	dspace
spelling	oai:doaj.org-article:11bcb034aa864a1d82d3645863352de82021-12-01T08:23:08ZThree-Dimensional Iron Oxide Nanoparticle-Based Contrast-Enhanced Magnetic Resonance Imaging for Characterization of Cerebral Arteriogenesis in the Mouse Neocortex1662-453X10.3389/fnins.2021.756577https://doaj.org/article/11bcb034aa864a1d82d3645863352de82021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnins.2021.756577/fullhttps://doaj.org/toc/1662-453XPurpose: Subsurface blood vessels in the cerebral cortex have been identified as a bottleneck in cerebral perfusion with the potential for collateral remodeling. However, valid techniques for non-invasive, longitudinal characterization of neocortical microvessels are still lacking. In this study, we validated contrast-enhanced magnetic resonance imaging (CE-MRI) for in vivo characterization of vascular changes in a model of spontaneous collateral outgrowth following chronic cerebral hypoperfusion.Methods: C57BL/6J mice were randomly assigned to unilateral internal carotid artery occlusion or sham surgery and after 21 days, CE-MRI based on T2*-weighted imaging was performed using ultra-small superparamagnetic iron oxide nanoparticles to obtain subtraction angiographies and steady-state cerebral blood volume (ss-CBV) maps. First pass dynamic susceptibility contrast MRI (DSC-MRI) was performed for internal validation of ss-CBV. Further validation at the histological level was provided by ex vivo serial two-photon tomography (STP).Results: Qualitatively, an increase in vessel density was observed on CE-MRI subtraction angiographies following occlusion; however, a quantitative vessel tracing analysis was prone to errors in our model. Measurements of ss-CBV reliably identified an increase in cortical vasculature, validated by DSC-MRI and STP.Conclusion: Iron oxide nanoparticle-based ss-CBV serves as a robust, non-invasive imaging surrogate marker for neocortical vessels, with the potential to reduce and refine preclinical models targeting the development and outgrowth of cerebral collateralization.Till de BortoliTill de BortoliPhilipp Boehm-SturmPhilipp Boehm-SturmPhilipp Boehm-SturmStefan P. KochStefan P. KochStefan P. KochMelina Nieminen-KelhäMelina Nieminen-KelhäLars WesselsLars WesselsSusanne MuellerSusanne MuellerSusanne MuellerGiovanna D. IelacquaJan KlohsPeter VajkoczyPeter VajkoczyNils HechtNils HechtFrontiers Media S.A.articlecontrast-enhanced MRIcerebral blood volumeiron oxide nanoparticlesstrokecerebrovascular diseasevessel imagingNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Neuroscience, Vol 15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	contrast-enhanced MRI cerebral blood volume iron oxide nanoparticles stroke cerebrovascular disease vessel imaging Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	contrast-enhanced MRI cerebral blood volume iron oxide nanoparticles stroke cerebrovascular disease vessel imaging Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Till de Bortoli Till de Bortoli Philipp Boehm-Sturm Philipp Boehm-Sturm Philipp Boehm-Sturm Stefan P. Koch Stefan P. Koch Stefan P. Koch Melina Nieminen-Kelhä Melina Nieminen-Kelhä Lars Wessels Lars Wessels Susanne Mueller Susanne Mueller Susanne Mueller Giovanna D. Ielacqua Jan Klohs Peter Vajkoczy Peter Vajkoczy Nils Hecht Nils Hecht Three-Dimensional Iron Oxide Nanoparticle-Based Contrast-Enhanced Magnetic Resonance Imaging for Characterization of Cerebral Arteriogenesis in the Mouse Neocortex
description	Purpose: Subsurface blood vessels in the cerebral cortex have been identified as a bottleneck in cerebral perfusion with the potential for collateral remodeling. However, valid techniques for non-invasive, longitudinal characterization of neocortical microvessels are still lacking. In this study, we validated contrast-enhanced magnetic resonance imaging (CE-MRI) for in vivo characterization of vascular changes in a model of spontaneous collateral outgrowth following chronic cerebral hypoperfusion.Methods: C57BL/6J mice were randomly assigned to unilateral internal carotid artery occlusion or sham surgery and after 21 days, CE-MRI based on T2*-weighted imaging was performed using ultra-small superparamagnetic iron oxide nanoparticles to obtain subtraction angiographies and steady-state cerebral blood volume (ss-CBV) maps. First pass dynamic susceptibility contrast MRI (DSC-MRI) was performed for internal validation of ss-CBV. Further validation at the histological level was provided by ex vivo serial two-photon tomography (STP).Results: Qualitatively, an increase in vessel density was observed on CE-MRI subtraction angiographies following occlusion; however, a quantitative vessel tracing analysis was prone to errors in our model. Measurements of ss-CBV reliably identified an increase in cortical vasculature, validated by DSC-MRI and STP.Conclusion: Iron oxide nanoparticle-based ss-CBV serves as a robust, non-invasive imaging surrogate marker for neocortical vessels, with the potential to reduce and refine preclinical models targeting the development and outgrowth of cerebral collateralization.
format	article
author	Till de Bortoli Till de Bortoli Philipp Boehm-Sturm Philipp Boehm-Sturm Philipp Boehm-Sturm Stefan P. Koch Stefan P. Koch Stefan P. Koch Melina Nieminen-Kelhä Melina Nieminen-Kelhä Lars Wessels Lars Wessels Susanne Mueller Susanne Mueller Susanne Mueller Giovanna D. Ielacqua Jan Klohs Peter Vajkoczy Peter Vajkoczy Nils Hecht Nils Hecht
author_facet	Till de Bortoli Till de Bortoli Philipp Boehm-Sturm Philipp Boehm-Sturm Philipp Boehm-Sturm Stefan P. Koch Stefan P. Koch Stefan P. Koch Melina Nieminen-Kelhä Melina Nieminen-Kelhä Lars Wessels Lars Wessels Susanne Mueller Susanne Mueller Susanne Mueller Giovanna D. Ielacqua Jan Klohs Peter Vajkoczy Peter Vajkoczy Nils Hecht Nils Hecht
author_sort	Till de Bortoli
title	Three-Dimensional Iron Oxide Nanoparticle-Based Contrast-Enhanced Magnetic Resonance Imaging for Characterization of Cerebral Arteriogenesis in the Mouse Neocortex
title_short	Three-Dimensional Iron Oxide Nanoparticle-Based Contrast-Enhanced Magnetic Resonance Imaging for Characterization of Cerebral Arteriogenesis in the Mouse Neocortex
title_full	Three-Dimensional Iron Oxide Nanoparticle-Based Contrast-Enhanced Magnetic Resonance Imaging for Characterization of Cerebral Arteriogenesis in the Mouse Neocortex
title_fullStr	Three-Dimensional Iron Oxide Nanoparticle-Based Contrast-Enhanced Magnetic Resonance Imaging for Characterization of Cerebral Arteriogenesis in the Mouse Neocortex
title_full_unstemmed	Three-Dimensional Iron Oxide Nanoparticle-Based Contrast-Enhanced Magnetic Resonance Imaging for Characterization of Cerebral Arteriogenesis in the Mouse Neocortex
title_sort	three-dimensional iron oxide nanoparticle-based contrast-enhanced magnetic resonance imaging for characterization of cerebral arteriogenesis in the mouse neocortex
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/11bcb034aa864a1d82d3645863352de8
work_keys_str_mv	AT tilldebortoli threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT tilldebortoli threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT philippboehmsturm threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT philippboehmsturm threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT philippboehmsturm threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT stefanpkoch threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT stefanpkoch threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT stefanpkoch threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT melinanieminenkelha threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT melinanieminenkelha threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT larswessels threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT larswessels threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT susannemueller threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT susannemueller threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT susannemueller threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT giovannadielacqua threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT janklohs threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT petervajkoczy threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT petervajkoczy threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT nilshecht threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex AT nilshecht threedimensionalironoxidenanoparticlebasedcontrastenhancedmagneticresonanceimagingforcharacterizationofcerebralarteriogenesisinthemouseneocortex
_version_	1718405338509606912

Three-Dimensional Iron Oxide Nanoparticle-Based Contrast-Enhanced Magnetic Resonance Imaging for Characterization of Cerebral Arteriogenesis in the Mouse Neocortex

Ejemplares similares