Culturing and Scaling up Stem Cells of Dental Pulp Origin Using Microcarriers

Ectomesenchymal stem cells derived from the dental pulp are of neural crest origin, and as such are promising sources for cell therapy and tissue engineering. For safe upscaling of these cells, microcarrier-based culturing under dynamic conditions is a promising technology. We tested the suitability...

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Autores principales:	Anna Földes, Hajnalka Reider, Anita Varga, Krisztina S. Nagy, Katalin Perczel-Kovach, Katalin Kis-Petik, Pamela DenBesten, András Ballagi, Gábor Varga
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	stem cells mesenchymal scaffold Cytodex 1 Cytopore 2 dental pulp Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/11c0602b7ab34e51a5d6ff23aa0e9033
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spelling	oai:doaj.org-article:11c0602b7ab34e51a5d6ff23aa0e90332021-11-25T18:48:42ZCulturing and Scaling up Stem Cells of Dental Pulp Origin Using Microcarriers10.3390/polym132239512073-4360https://doaj.org/article/11c0602b7ab34e51a5d6ff23aa0e90332021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/22/3951https://doaj.org/toc/2073-4360Ectomesenchymal stem cells derived from the dental pulp are of neural crest origin, and as such are promising sources for cell therapy and tissue engineering. For safe upscaling of these cells, microcarrier-based culturing under dynamic conditions is a promising technology. We tested the suitability of two microcarriers, non-porous Cytodex 1 and porous Cytopore 2, for culturing well characterized dental pulp stem cells (DPSCs) using a shake flask system. Human DPSCs were cultured on these microcarriers in 96-well plates, and further expanded in shake flasks for upscaling experiments. Cell viability was measured using the alamarBlue assay, while cell morphology was observed by conventional and two-photon microscopies. Glucose consumption of cells was detected by the glucose oxidase/Clark-electrode method. DPSCs adhered to and grew well on both microcarrier surfaces and were also found in the pores of the Cytopore 2. Cells grown in tissue culture plates (static, non-shaking conditions) yielded 7 × 10<sup>5</sup> cells/well. In shake flasks, static preincubation promoted cell adhesion to the microcarriers. Under dynamic culture conditions (shaking) 3 × 10<sup>7</sup> cells were obtained in shake flasks. The DPSCs exhausted their glucose supply from the medium by day seven even with partial batch-feeding. In conclusion, both non-porous and porous microcarriers are suitable for upscaling ectomesenchymal DPSCs under dynamic culture conditions.Anna FöldesHajnalka ReiderAnita VargaKrisztina S. NagyKatalin Perczel-KovachKatalin Kis-PetikPamela DenBestenAndrás BallagiGábor VargaMDPI AGarticlestem cellsmesenchymalscaffoldCytodex 1Cytopore 2dental pulpOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3951, p 3951 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	stem cells mesenchymal scaffold Cytodex 1 Cytopore 2 dental pulp Organic chemistry QD241-441
spellingShingle	stem cells mesenchymal scaffold Cytodex 1 Cytopore 2 dental pulp Organic chemistry QD241-441 Anna Földes Hajnalka Reider Anita Varga Krisztina S. Nagy Katalin Perczel-Kovach Katalin Kis-Petik Pamela DenBesten András Ballagi Gábor Varga Culturing and Scaling up Stem Cells of Dental Pulp Origin Using Microcarriers
description	Ectomesenchymal stem cells derived from the dental pulp are of neural crest origin, and as such are promising sources for cell therapy and tissue engineering. For safe upscaling of these cells, microcarrier-based culturing under dynamic conditions is a promising technology. We tested the suitability of two microcarriers, non-porous Cytodex 1 and porous Cytopore 2, for culturing well characterized dental pulp stem cells (DPSCs) using a shake flask system. Human DPSCs were cultured on these microcarriers in 96-well plates, and further expanded in shake flasks for upscaling experiments. Cell viability was measured using the alamarBlue assay, while cell morphology was observed by conventional and two-photon microscopies. Glucose consumption of cells was detected by the glucose oxidase/Clark-electrode method. DPSCs adhered to and grew well on both microcarrier surfaces and were also found in the pores of the Cytopore 2. Cells grown in tissue culture plates (static, non-shaking conditions) yielded 7 × 10<sup>5</sup> cells/well. In shake flasks, static preincubation promoted cell adhesion to the microcarriers. Under dynamic culture conditions (shaking) 3 × 10<sup>7</sup> cells were obtained in shake flasks. The DPSCs exhausted their glucose supply from the medium by day seven even with partial batch-feeding. In conclusion, both non-porous and porous microcarriers are suitable for upscaling ectomesenchymal DPSCs under dynamic culture conditions.
format	article
author	Anna Földes Hajnalka Reider Anita Varga Krisztina S. Nagy Katalin Perczel-Kovach Katalin Kis-Petik Pamela DenBesten András Ballagi Gábor Varga
author_facet	Anna Földes Hajnalka Reider Anita Varga Krisztina S. Nagy Katalin Perczel-Kovach Katalin Kis-Petik Pamela DenBesten András Ballagi Gábor Varga
author_sort	Anna Földes
title	Culturing and Scaling up Stem Cells of Dental Pulp Origin Using Microcarriers
title_short	Culturing and Scaling up Stem Cells of Dental Pulp Origin Using Microcarriers
title_full	Culturing and Scaling up Stem Cells of Dental Pulp Origin Using Microcarriers
title_fullStr	Culturing and Scaling up Stem Cells of Dental Pulp Origin Using Microcarriers
title_full_unstemmed	Culturing and Scaling up Stem Cells of Dental Pulp Origin Using Microcarriers
title_sort	culturing and scaling up stem cells of dental pulp origin using microcarriers
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/11c0602b7ab34e51a5d6ff23aa0e9033
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Culturing and Scaling up Stem Cells of Dental Pulp Origin Using Microcarriers

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