E2F transcription factor 1 is involved in the phenotypic modulation of esophageal squamous cell carcinoma cells via microRNA-375

E2F family of transcription factors modulates multiple cellular functions associated with cell cycle and apoptosis. Here, we focused on the relevance of E2F1 to esophageal squamous cell carcinoma (ESCC) and identification of E2F1-mediated network in this study. Query of Gene Expression Omnibus datab...

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Autores principales:	Pengfei Li, Huina Lv, Yongkai Wu, Ke Xu, Min Xu, Yegang Ma
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	esophageal squamous cell carcinoma e2f1 microrna-375 sesn3 the pi3k/akt pathway Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/11c46c5a5bac4d8981e3a789dcd4023b
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spelling	oai:doaj.org-article:11c46c5a5bac4d8981e3a789dcd4023b2021-11-04T15:51:54ZE2F transcription factor 1 is involved in the phenotypic modulation of esophageal squamous cell carcinoma cells via microRNA-3752165-59792165-598710.1080/21655979.2021.1996510https://doaj.org/article/11c46c5a5bac4d8981e3a789dcd4023b2021-10-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1996510https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987E2F family of transcription factors modulates multiple cellular functions associated with cell cycle and apoptosis. Here, we focused on the relevance of E2F1 to esophageal squamous cell carcinoma (ESCC) and identification of E2F1-mediated network in this study. Query of Gene Expression Omnibus database revealed that E2F1 was the core gene that was upregulated in ESCC. E2F1 downregulation inhibited ESCC cell activity. microRNA (miR)-375 was confirmed to be a downstream target of E2F1. E2F1 bound to miR-375 promoter and inhibited miR-375 transcription. Moreover, miR-375 inhibitor mitigated the repressive impacts of si-E2F1 on ESCC cells in part. Further study showed that sestrin 3 (SESN3) could interact with miR-375, and its knockdown annulled the stimulative effect of miR-375 inhibitor on ESCC development. Finally, E2F1 and SESN3 downregulation inhibited the phosphatidylinositol 3 kinase (PI3K)/AKT pathway activity in cells, while miR-375 inhibitor promoted PI3K/AKT pathway activation. These findings suggest that E2F1 inhibited miR-375 expression and promoted SESN3 expression to activate the PI3K/AKT pathway in ESCC.Pengfei LiHuina LvYongkai WuKe XuMin XuYegang MaTaylor & Francis Grouparticleesophageal squamous cell carcinomae2f1microrna-375sesn3the pi3k/akt pathwayBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	esophageal squamous cell carcinoma e2f1 microrna-375 sesn3 the pi3k/akt pathway Biotechnology TP248.13-248.65
spellingShingle	esophageal squamous cell carcinoma e2f1 microrna-375 sesn3 the pi3k/akt pathway Biotechnology TP248.13-248.65 Pengfei Li Huina Lv Yongkai Wu Ke Xu Min Xu Yegang Ma E2F transcription factor 1 is involved in the phenotypic modulation of esophageal squamous cell carcinoma cells via microRNA-375
description	E2F family of transcription factors modulates multiple cellular functions associated with cell cycle and apoptosis. Here, we focused on the relevance of E2F1 to esophageal squamous cell carcinoma (ESCC) and identification of E2F1-mediated network in this study. Query of Gene Expression Omnibus database revealed that E2F1 was the core gene that was upregulated in ESCC. E2F1 downregulation inhibited ESCC cell activity. microRNA (miR)-375 was confirmed to be a downstream target of E2F1. E2F1 bound to miR-375 promoter and inhibited miR-375 transcription. Moreover, miR-375 inhibitor mitigated the repressive impacts of si-E2F1 on ESCC cells in part. Further study showed that sestrin 3 (SESN3) could interact with miR-375, and its knockdown annulled the stimulative effect of miR-375 inhibitor on ESCC development. Finally, E2F1 and SESN3 downregulation inhibited the phosphatidylinositol 3 kinase (PI3K)/AKT pathway activity in cells, while miR-375 inhibitor promoted PI3K/AKT pathway activation. These findings suggest that E2F1 inhibited miR-375 expression and promoted SESN3 expression to activate the PI3K/AKT pathway in ESCC.
format	article
author	Pengfei Li Huina Lv Yongkai Wu Ke Xu Min Xu Yegang Ma
author_facet	Pengfei Li Huina Lv Yongkai Wu Ke Xu Min Xu Yegang Ma
author_sort	Pengfei Li
title	E2F transcription factor 1 is involved in the phenotypic modulation of esophageal squamous cell carcinoma cells via microRNA-375
title_short	E2F transcription factor 1 is involved in the phenotypic modulation of esophageal squamous cell carcinoma cells via microRNA-375
title_full	E2F transcription factor 1 is involved in the phenotypic modulation of esophageal squamous cell carcinoma cells via microRNA-375
title_fullStr	E2F transcription factor 1 is involved in the phenotypic modulation of esophageal squamous cell carcinoma cells via microRNA-375
title_full_unstemmed	E2F transcription factor 1 is involved in the phenotypic modulation of esophageal squamous cell carcinoma cells via microRNA-375
title_sort	e2f transcription factor 1 is involved in the phenotypic modulation of esophageal squamous cell carcinoma cells via microrna-375
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/11c46c5a5bac4d8981e3a789dcd4023b
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E2F transcription factor 1 is involved in the phenotypic modulation of esophageal squamous cell carcinoma cells via microRNA-375

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